2. The next generation of
sequenching technologies
Ziebolz B, 2006, Next generation sequenching technologies, BTi.
3. Sequenching by
Hybridization
Prinsip:
Perbedaan hibridisasi dari pelacak oligonukleotidayang dapat digunakan untuk
membaca kode genetik dari DNA target.
Pendekatan:
1. Imobilisasi DNA pada membran yang akan menginisiasi hibridisasi yang
dideteksi oleh probe oligonukleotida pendek.
2. Hibridisasi DNA ke microfabricated tilling arrays immobilized
oligonucleotide, with > 100.000 copies of each induvidual of feature.
4. Mekanisme SBH
To resequence a given base, four features are present on the microarray, each identical except for a different
nucleotide at the query position (the central base of 25-bp oligonucleotides). Genotyping data at each base
are obtained through the differential hybridization of genomic DNA to each set of four features.
Jay Shendure*, Robi D.Mitra‡, Chris Varma* and George M. Church*, 2005, ADVANCED SEQUENCING TECHNOLOGIES: METHODS AND GOALS, Nature Review, Volume 5.
6. MIcroarray
DNA microarray analysis is one of the fastest-growing new technologies in the field of
genetic research. The basis of DNA microarray is sequencing by hybridization.
