1. Give three possible reasons why membrane proteins might diffuse more slowly than expected, or not at all. 2. Describe the use of the mouse-human hybrid cells, FRAP, and STP in studying lateral diffusion of membrane proteins. 3. Many cells are polar. For example, intestinal epithelial cells have one side that faces the lumen of the intestine, and the other side that is attached to the basement membrane. These different sides contain different membrane proteins. - In an intestinal epithelial cell, what functions occur at the apical membrane? The lateral membrane? The basal membrane? - How might the proteins specific to these areas be kept in their proper locations? 4. The lipid membrane has a cytoskeleton that supports membrane structure. Describe the roles of Spectrin, Ankyrin, and Band 3 protein in this support structure. Solution 1). The membrane proteins might diffuse more slowly than expected because, (i). They have large molecular size (molecules of smaller size are more likely to diffuse through the membrane) (ii). They may be charged molecules (charged molecules do not pass through the membrane) (iii). They are hydrophilic in nature (uncharged or non-charged molecules pass through the membrane).