describe two properties of iPS and ES cells, including the meaning i.pdf

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Consider the RC circuit shown below: What is the characteristic time constant of the circuit? What is the maximum charge on the capacitor? How long does it take after the switch is closed to charge the capacitor to within 63% of its maximum charge Solution a) Time constant is given by, T = R*C = 100*3.2*10^-6 = 3.2*10^-4 s b\') maximum charge, Qo = C*Vmax = 3.2*10^-6*15 = 4.8*10^-5 C c) The chrarge on the capacitor is given by : Q = Qo*(1 - e^(-t/RC)) Now, Q = 63 percent of Qo = 0.63*Qo So, 0.63*Qo = Qo*(1 - e^(-t/RC)) = e^(-t/RC) = 0.37 So, -t/RC = ln(0.37) = -0.994 So, t = RC*0.994 = 3.2*10^-4*0.994 = 3.18*10^-4 s <--------answer.

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describe two properties of iPS and ES cells, including the meaning if the term pluripotent
Solution
Pluripotent cells are those cells that has the ability to give rise to all types of the cells that make
up the body of a organism. If the pluripotent cells are produced directly from the adult cells, they
are called Induced Pluripotent cells (iPS). The pluripotent stem cells derived from the inner cell
mass of a blastocyst, of an embryo is called Embryonic stem cells (ES cells).
Properties :
1. iPS cells and ES cells shows identical properties. Both expresses the pluripotent cell marker
gene such as Eras, Rex1 ad Esg1. They also shows epigenetic status including DNA methylation
and histone modification.
2. They produce flat colonies with tightly packed cels having high nucleo-cytoplasmic ratios,
defined borders and prominent nucleoli. Both cells can maintain telomere length which clearly
indicate the ability of these cells,to undergo unlimited cell proliferation in vitro.

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