c) All colonies sub-cultured from blood culture broth media must be identified. Multiple bottles improves the likelihood that diphtheroids and coagulase negative Staphylococcus will be treated. During sepsis, multiple organsims may be present in the infection. Time separation and culture of blood in different environement allows different types of bacteria to be easily separated and identified. Solution c) All colonies sub-cultured from blood culture broth media must be identified. Multiple bottles improves the likelihood that diphtheroids and coagulase negative Staphylococcus will be treated. During sepsis, multiple organsims may be present in the infection. Time separation and culture of blood in different environement allows different types of bacteria to be easily separated and identified..