2. Purpose: induction of an immune response to
achieve protection against an antigen to protect
a future challenge or to initiate a reaction
against already present antigent
Method: Injection or oral uptake( e.g. Protein,
toxin, attenuated pathogen etc.) together with
adjuvant or carrier
3. DNA vaccines are made of a modified form of
an infectious organism's DNA
microbe DNA( not human) is inserted into
plasmid vector
when injected into a person(intramuscular
injection, intradermal saline injection) -leads to
the expression of the genes within this
modified, foreign DNA
result, the person's immune system responds in
both antibody and cell mediated
4. when injected into a person -leads to the
expression of the genes within this modified,
foreign DNA
result, the person's immune system responds in
a protective manner
Human cells take up some plasmids and
express microbial DNA in the form of protein
5. Gene gun: initiates responses by transfected or
antigen bearing epidermal Langerhans cells
that move in lymph from bombarded skin to
the draining lymph nodes
Intramuscular injection: the functional DNA
appears to move as free through blood to
spleen where professional APC initiate
responses
