3. Introduction
Principles of plant tissue culture :
Tissue culture simply directs and assistants the natural
potential within the plant to put forth new growth and
the multiply in highly efficiently and predictable way.
Totipotency i.e is the capacity of an individual all to
regenerate in to the whole plant, the concept of
totipotency (T.H. Morgan, 1901).
All the plant cells have their property since potential
lies mainly in cellular differentiation.This indicates that
all genes responsible for differentiation tissue or organ
are able to express only under adequate culture
conditions.
4. Introduction
The three main changes or stages is the complete development an
ordered change or progress often towards a higher more complex
state of a cell are
Cell division
Cell elongation
Cell Maturation
Two kinds of plant growth are possible in vitro
Organized Growth : Occurs either when organized plant parts or organs
such as the growing point apical Meristem of shoots or roots leaf initials,
young flower buds and small fruit are transferred to culture (where they
may continue to grow with their structure preserved ) or when these
structure are formed afresh during the culture of unorganized tissues.
Unorganized Growth : Occurs when pieces of whole plant are cultured in
vitro. The tissue thus formed typically lack any recognizable structure
contain only limited no of the many different kinds of specialized cells
found in an intact plant.
5.
6. Basic TechniquesBasic Techniques
Setting up of a tissue culture lab requires proper planning.
It is divided into 5 areas
Media preparation room
Aseptic transfer area
Culture room
Analytical room
Acclimatization room
7. Media Preparation RoomMedia Preparation Room
Refrigerator & freezer
Water purification & storage system
Glassware washing facility
Continuous supply of single & double distilled water
Culture media, washing powder, disinfectants
Cabinets or shelves
8. Aseptic Transfer AreaAseptic Transfer Area
Laminar air flow
Dissecting microscopes
Dissection instruments
Gas outlet
Vacuum facility
Sterilizer
9. Culture RoomCulture Room
Environmentally controlled
Incubators with controlled temperature
Rotary shakers
Lux meter
Space for cultures requiring complete darkness
14. MediaMedia
No single medium supports growth of all tissues.
Some basic factors
Callus induction
Organogenesis
Murashige-Skoog medium,White’s medium, woody plant
medium
59. Refrences
A text book of Biotechnology: R.C.Dubey
PlantTissue Culture: S.S.Purohit
www.wikipedia.org
Pharmacognosy-III, sem -5 by Dr.A.M.Baheti and Mr. R.K.
Dumbre, pg no. 2-2 (GTU syllabus)