This document presents an industrial presentation on plant tissue culture. It introduces plant tissue culture as a process to generate entire plantlets under controlled conditions using plant parts. The basic apparatus needed for a plant tissue culture lab is described, including hot air ovens, autoclaves, laminar airflow hoods, and basic media like MS media. The basic protocol of plant tissue culture is outlined, including sterilization, media preparation, explant inoculation, incubation, and subculture. Aloe vera and potato were cultured as examples - aloe vera callus growth was observed after 40-45 days and potato growth after 45 days.

1. INDUSTTRIAL
PRESENTATION
PLANT TISSUE CULTURE
BY:
VIVEK KUMAR CHAURASIA
BT (VII) SEMESTER
1306854073

2. CONTENT
‽ Introduction about Plant Tissue Culture
‽ Basic Apparatus needed for PTC Lab
‽ Basic Media in PTC : MS Media
‽ Basic Protocol of Plant Tissue Culture
‽ Plants Cultured:
a. Aloe Vera
b. Potato
‽ Results
‽ Bibliography
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3. INTRODUCTION TO PTC
Plant Tissue Culture (PTC) is the field of science in which we generate an entire plantlet under
the controlled conditions (in vitro) and then introduced in the environment.
It’s a rapid process as compared to the conventional plant growing procedure and any plant part
can be used to generate the entire plant.
The property of the plant which is the principle of the PTC is totipotency (ability to generate
entire plant from a single part)
However, not all plant cells show totipotency, therefore cells from meristem or younger cells are
used for producing culture.
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4. APPARATUS NEEDED FOR
PTC LAB
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5. 5
Hot Air Oven (For
glassware sterilization)
Magnetic Stirrer (for mixing media
components)

6. 6
pH balance (for maintaining the pH )
Autoclave (for sterilizing the media)

7. 7
Laminar air flow (for inoculation and sub culturing of plantlets)

8. BASIC MEDIA : MS
MEDIA
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10. BASIC PROTOCOL OF PTC

11. •Sterilization of glasswares (jars,beakers, test tubes etc)
• The glasswares are washed and are then either dried in oven or autoclaved
•Preparation of media
•Mostly MS media is used with some modifications
•Sterilization of media (Autoclaving of media )
•Thermo labile components should be sterilized separately through filtration and then added later
•Washing of explant
•This includes washing explant with tap water, distilled water, detergent, ethanol
•Inoculation of explant in the media
•The inoculation is done in the laminar air flow under sterile conditions
•Incubation
•The jars or test tubes after inoculation are kept in the growth room under optimal temperature,
light (photoperiod), humidity etc to support the growth of the explant
•Sub Culturing
•The explants on which growth is noticed are sub cultured i.e. transferred to different media jars.
This ensures the nutrients for the growing plantlet.
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12. PLANTS CULTURED
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13. ALOE VERA
Sceintific name : Aloe barbadensis miller.
Explant used : Young axillary shoots
Media Used : BAP media
Conditions : 22± 2˚ C, 16/8 hours photoperiod
Of incubation
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14. Inoculated jars of aloe vera
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16. POTATO
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Scientific name : Solanum tuberosum
Explant used : Eye of the potato (tuber)
Media used : MS media
Conditions : 18± 2˚ C, 18 hours photoperiod
Of Incubation

17. Inoculated jars of potato tubers
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18. RESULTS

19. After 40-45 days of inoculation, the callus growth was
Observed in the Aloe Vera jars
These callus were then sub cultured in the new jars.
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20. After 45 days of culture, the growth was observed in potato jars.
They were subsequently sub cultured
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21. BIBLIOGRAPHY
www.slideshare.net
www.Wikipedia.org
www.pubmed.com
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22. THANK YOU
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