Tuesday theme 1 1050 1105 large briefing room solynta

1. Diploid hybrid potato breeding for Africa
APA Conference, Addis Ababa
October 11th, 2016
Pim Lindhout
www.solynta.com

2. EAPR meeting, OULU, Finland
Theo Schotte, breeding
Hein Kruyt,
business
development
Johan Trouw,
Seed producti
Pim Lindhout, R&D
Solynta founders
Former board of De Ruiter Seeds

3. EAPR meeting, OULU, Finland
Solynta staff in 2016

4. Hybrid breeding

5. Female germplasm pool Male germplasm pool
Elite female lines Elite male lines
F1 test crosses
Female parent lines Male parent lines
line selectionline selection
hybrid
performance
selection
hybrid
performance
selection
3 years
2 years
Diploid Potato
germplasm
Diploid
F1 hybrid
cultivar
Diploid F1 hybrid breeding

6. Female parent line Male parent line
Diploid
F1 hybrid
cultivar
Female parent line Male parent line
Commercial seed of F1 hybrid cultivar
Maintainance
by seedMaintainance
by seed
crossing
large scale
crossing
1 year
F1 hybrid seed production

7. Traditional breeding Hybrid breeding
Introduction one trait 15 – 50 years 2 – 3 years
Phytophthora resistance > 50 years 4 years
Multiplication vegetative (5 - 10 y) seeds (one season)
Seed health contaminated clean
Advantages of hybrid breeding in potato
Conclusion Russet Burbank is leading
variety for 145 years
Dynamic introductions
of innovative products
Value creation
Disruptive change

8. Corn as example for F1 hybrid breeding
Potato today

9. 400.000 plants = 10 ha commercial potato field
~ 200 gram seeds = envelope
~ 25 ton seed tubers = big sea container

10. Homozygocity increase by selfing is much
faster in diploids as compared to tetraploids

11. Two barriers:
1. Selfincompatibility
2. Inbreeding depression

12. EAPR meeting, OULU, Finland
Diploid self-compatible plants

13. EAPR meeting, OULU, Finland
Selfcompatible vigourous F7 plant

14. Two barriers:
1. Selfincompatibility
2. Inbreeding depression

15. Marker assisted breeding
Stacking resistance genes to
Phytophthora infestans

16. Female parent line Male parent line
F1 hybrid cultivarIntroduction new
trait by crossing,
backcrossing,
selfing and
selection
Improved female parent line Male parent line
Improved F1 hybrid cultivar
with new trait
Maintainance
by seed
crossing
crossing
2 years
1 year
Improved F1 hybrid breeding

17. Background genome composition
of resistant BC1 plant
0.5
2.6
11.4
30.1
52.3
60.0
71.2
86.4
01
4.8
25.5
33.3
40.4
45.1
48.2
02
2.9
35.6
45.9
54.6
58.2
03
5.2
14.7
50.2
60.2
69.7
04
1.4
7.7
12.7
20.3
40.5
51.7
05
5.9
8.7
32.1
40.9
58.0
06
4.9
31.6
51.5
55.9
07
3.2
8.5
36.1
43.4
56.8
08
5.5
10.5
21.2
33.7
44.0
54.1
58.7
09
3.8
14.8
20.1
36.2
46.1
47.1
50.8
52.2
54.2
55.7
58.6
10
14.2
41.2
45.2
11
6.1
11.7
60.0
12
R-gene

18. Objective for 2017:
Double stack
Phytophthora resistant varieties

19. What about hybrid breeding?

20. 2009: Proof of principle F1 hybrid breeding

21. 2009 - 2015: developing good
performing inbred lines
2015: first serious crosses to
produce experimental hybrids
2016: upscaling breeding

22. EAPR meeting, OULU, FinlandSeed boxes in greenhouse

23. Seedlings in greenhouse

24. Seedlings planting in the field

25. EAPR meeting, OULU, Finland
Seedlings in the field

26. Harvesting tubers

27. Yield of experimental hybrids from seedlings
grown for 90 days in the field

28. First potato hybrids, raised from seedling
in Africa (East-DRC)
Presentation Michiel de Vries at PWC Roundtable

29. Graham Thiele, APA 2016:
”Faster, better, smarter”
Solution: hybrid breeding

30. Graham Thiele, APA 2016:
“Climate smart research and breeding”

31. Unite forces of CIP and Solynta to develop
climate resilient potato systems for Africa

32. Future of potato breeding is in hybrid seeds