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WELCOME
Exploitation
of Male
Sterility in
Monocots
and Dicots
INTRODUCTION
Male sterility is defined as an absence or non-
function of pollen grain in plant or incapability
of plants to produce or release functional
pollen grains.
Why to Exploit Male Sterility in
Plants??
• Eliminate emasculation in hybridization.
• Increase natural cross-pollination in self
pollinated species.
• Controlled pollination plants.
• Facilitate commercial hybrid seed production.
Exploitation of Male sterility in Rice
In rice following types of male sterility systems
are used
 Cytoplasmic Male Sterility( Three line
breeding)
 Genetic Male Sterility( Two line breeding).
 Male sterility induced by Chemical
Hybridizing Agents.
Ppt on exploitation of male sterility in monocots and dicots
Cytoplasmic Male Sterility in rice
• Male Sterility is governed by cytoplasmic
genes or plasmagenes.
• Source of Male Sterility.
WA-CMS lines
• WA-CMS lines are the most widely deployed lines in hybrid
rice production .
• Pollen abortion in WA-CMS occurs relatively early during
microspore development, mainly at the uninucleate stage
(Luo et al.2013), resulting in amorphous aborted pollen
grains.
• The pollen abortion is determined by the genotype of
sporophytic tissues, not by the genotype of the pollen itself.
• Efforts are made to transfer this WA cytosterility into
genetic background of elite breeding lines.
Treatment with 1% Pottassium iodide
a) Stained pollens- viable pollen
b) Unstained pollens - non viable pollen
CMS Line Developed At Elite line
converted
Origin of
elite line
IR 46826 A IRRI IR 10154-23-3-3 IRRI
IR 46827 A IRRI IR 10176-24-6-2 IRRI
IR 46828 A IRRI IR 10179-2-3-1 IRRI
IR 46829 A IRRI IR 19792-15-2-3-3 IRRI
IR 46831 A IRRI Jikkoku Seranai 52-37 India
IR 46883 A IRRI MR-365 India
IR 46885 A IRRI PAU 269-1-8-4-1-1-1 India
Madhu A CRRI ,India Madhu India
HR 7017 A Iri Korea Samkangbyeo Korea
Source: Ram H., H.(2012). Crop Breeding and Biotechnology. Chapter 2,96- 98.
Procedure of utilization of CMS line
in Seed production
Hybrids developed using CMS in India
Hybrid Parentage
APRH- 1 IR 58025 A x Vajram
APRH-2 IR 62829 A x MTU 9992
MGR- 1 IR 62829 A x 10198-66-2R
KRH-1 IR 58025 A x IR 9761-10-IR
CNRH-3 IR 62829 A x Ajaya R
DRRH-1 IR 58025 A x IR 40750-82-2-2-3R
KRH-2 IR 58025 A x KMR 3
Pant Shankar Dhan 1 IR 58025 A x UPRI 192-133R
CORH 2 IR 58025 A x C 20 R
ADTRH 1 IR 58025 A x IR66
Sahyadri IR 58025 A x BR 827-35-3-1-1-R
Narendra Sankar Dhan 2 IR 58025 A x NDR 3026-3-I-R
Source: Ram H., H.(2012). Crop Breeding and Biotechnology. Chapter 2,96- 98.
Why There is Switch over from three
line to two line breeding??
• Cytoplasmic male sterility- fertility restoration system
based on three line breeding is often difficult to
maintain.
• Problems in the maintenance of A line.
• Lack of diversity in A and R lines, and the presence
of minor fertility genes in B lines ,lead to low
heterotic potential and high seed production costs.
• Hence two line breeding emerged.
Exploitation of GMS in Rice
• Male sterility is governed by nuclear genes.
• In GMS ,the GMS line produce 50% of male fertile
plants and 50% male sterile plants.
• So its very much necessary to identify and eliminate
male fertile plants before pollen shed .
• Thus this draw back of GMS is overcome by using
Photoperiod Sensitive GMS.
Temperature sensitive GMS.
Procedure of utilization of TGMS/PGMS line
in Seed production
Exploitation of TGMS in Rice
Consider the Temperature/ Thermo Sensitive Genetic
Male Sterile line 5460S.
TGMS line Temperature Expression
5460S
< 28 ᵒC Complete
male fertility
> 30ᵒC Complete
male sterility
Source: Singh, B, D.(2012). Plant Breeding , Principles and Methods. Chapter
22,353- 355.
Maintenance of TGMS line in rice
LOCATION A
(< 28 ᵒC Temperature)
TGMS line (5460S.)
selfing
TGMS line
(Complete fertile)
Source: Singh, B, D.(2012). Plant Breeding , Principles and Methods. Chapter
22,353- 355.
Utilization of TGMS line in Hybrid
Seed Production
LOCATION B
(> 30ᵒC Temperature)
TGMS line (5460S.) x Male fertile line
Hybrid Seed
(Produced on TGMS line )
Source: Singh, B, D.(2012). Plant Breeding , Principles and Methods. Chapter
22,353- 355.
List of PGMS lines in Rice
Genetic Control lines Origin Expression of
MS
Controlled by two
recessive genes
PGMS lines
Nongken 58S Spontaneous
mutation
Day length shorter
than 13.75hr
X88 Hybridization Day length shorter
than 13hr
MG 201 EMS Mutagenesis Day length 12hr
Source: Singh, B, D.(2012). Plant Breeding , Principles and Methods. Chapter 6,90.
List of TGMS lines in Rice
Genetic Control lines Origin Expression of MS
Controlled by single
recessive gene
Annong- 1s Spontaneous mutation Temperature of 27 ᵒC
Hennong S Hybridization Temperature < 29ᵒC
5460S Irradiation Temperature >30ᵒC
SM-38 Spontaneous mutation Temperature < 22ᵒC
SM-5 Spontaneous mutation Temperature < 22ᵒC
JP-2 Spontaneous mutation Temperature < 26ᵒC
JP-38S Spontaneous mutation Temperature > 30ᵒC
Source: Singh, B, D.(2012). Plant Breeding , Principles and Methods. Chapter 6,90.
Hybrids developed using PGMS in
Rice
Hybrid Parentage
Hunan Pei ai 64s x Tequing
Anhui 7001s x Xiusui 04
Hubei 7001s x 1514
Guangdong Pei ai 64s x Shuanging11
Sichuan Pei ai 64s x Shuangingyou 1
Source: Ram H., H.(2012). Crop Breeding and Biotechnology. Chapter 2,100.
Exploitation of Male Sterility by
CHA’s in Rice
 Application of chemicals at specific growth
stage of crop results in chemically induced
male sterility and chemical components used
are called as Chemical Hybridizing agents.
 Two arsenical CHA’s are used in Rice
MG1( based on zinc methyl arsenate)
MG2 (based on sodium methyl arsenate)
CHA’s in Hybrid seed Production in
Rice
• Male sterility is artificially induced by spraying
gametocides to cause stamen sterility without
harming the pistil.
• The chemical which makes sterile the stamen, plant
can be used as female parent for producing hybrid
seed.
• Two selected lines are planted in alternate strips and
one is utilized as female (chemically sterilized) and is
pollinated by the other line, for production of hybrid
seed.
Rice hybrids based on CHA’s in rice
• Qing – Hua- Fu- Gwi.
• Gang – Hua- Qing – Lan.
• Gang – Hua- 2.
• You- Za-1.
• Ya- You-2.
• These are produced in china using arsenical
CHA’s.
Source: Singh, B, D.(2012). Plant Breeding , Principles and Methods. Chapter 22,355.
Exploitation of MS in Maize
CMS in Maize
• Three main CMS types have been described:
 CMS-T (Texas) (Rogers and Edwardson, 1952)
• Fertility restoration is sporophytic
• Rf1 (chr. 3) & Rf2(chr.9) are responsible for fertility restoration
 CMS-C (Charrua) (Beckett, 1971)
Fertility restoration is Sporophytic
•Rf4, Rf5, Rf6 are responsible for fertility restoration
 CMS-S (USDA) (Jones,1957)
• Fertility restoration is Gametophytic
• Rf3 (chr. 2) are responsible for fertility restoration
Fertility restoration in maize
T cytoplasm in maize
• Texas (T) cytoplasmic male sterility
discovered in 1940s.
• used extensively throughout the 1960s.
• Highly stable under all environmental
conditions.
• Characterized by failure of anther exertion
and pollen abortion.
• Plants bearing the T cytoplasm- susceptible to race T
of the southern corn leaf blight - (Cochliobolus
heterostrophus = Bipolaris maydis)
• Widespread use of T-cytoplasm for hybrid corn
production led to epidemic in 1970 in USA, with the
widespread rise of Race T of the southern corn leaf
blight .
• Thus utilization of T cytoplasm as a source of Male
sterility was stopped and detasseling is being used to
get male sterile plants.
Failure of T cytoplasm in maize
Source: Charles S, L.(1990). The Texas Cytoplasm of Maize. Cytoplasmic Male
sterility and Disease susceptibility. Science. Vol(250), 942-947.
Exploitation of Male sterility in Bajra
• The discovery of cytoplasmic-genic male-sterility
often called cytoplasmic male-sterility and the
development of male-sterile line Tift 23A1 (Burton
1958, 1965) laid the foundation of the pearl millet
(Penniseturn americanum) hybrid seed industry in
India.
• The first commercial hybrid was HB 1.
• It was bred on Tift 23A1 at the Punjab Agricultural
University (PAU), Ludhiana, India, and it showed a
100% yield average over the open-pollinated check
varieties (Athwal 1966).
• The A, cytoplasm of Tift 23A1 has been extensively utilized in
breeding a wide range of male-sterile lines.
• At Tifton, four male-sterile lines were produced either by
transferring single genes for specific traits into Tift 23A1 and
Tift 23B, or by backcrossing Non restorers into Tift 23A1
cytoplasm.
• Of these, mostly Tift 23A1, and to some extent Tift 23DA,
were used in India for hybrid production.
• Hybrids on Tift 23A1 yielded more than those on Tift 23DA1.
• Five hybrids on Tift 23A1 were released in quick succession
between 1965-1972, but all went out of cultivation within
about 5 years of their release due to high downy mildew
susceptibility.
Source of Cytoplasmic Male sterility in
Bajra
Cytoplasm Source CMS line Remarks
A1(Tifton) Mutation Tift 23 A, Tift 23 D2 A Used for
commercial
cultivation
A2 (Ludhiana) Mutation L66A Incomplete
expression of
MS
A3 P. Violaceum L67A Extremely stable
for male sterility
,very promising
restores in bajra
A4 Half sib pool of
the early gene
pool
ICMA-90111 Developed at
ICRISAT
Hybrids in Bajra
Hybrid Parentage
HB1 Tift 23 A x BIL 3B
HB2 Tift 23 A x J88
HB3 Tift 23 A X J104
HB4 Tift 23 A x K 560
HB5 Tift 23 A x K559
Source: Ram H., H.(2012). Crop Breeding and Biotechnology. Chapter 6,187- 209.
Exploitation of Male sterility in
Sunflower
• MS system used is Cytoplasmic Genetic Male
sterility system.
• Here both cytoplasmic genes and nuclear
genes govern the Male sterility.
• Source of male sterility in sunflower is CMS-
PET1 cytoplasm.
• CMS-PET1 cytoplasm of sunflower arose
from an interspecific cross between Helianthus
petiolaris and H. annuus.
Utilization of CMS PET Cytoplasm in
seed production
Ppt on exploitation of male sterility in monocots and dicots
Ppt on exploitation of male sterility in monocots and dicots
Hybrids developed Using CGMS
system in Sunflower
• Armavirtsky 3497
• BSH1
• BSH2
• BSH3 PET Cytoplasm.
• BSH4 (source of CMS)
• BSH5
• BSH6
• BSH8.
• Out of this BSH 1 and BSH2 found to be promising hybrids
for seed production.
• First Sunflower hybrid BSH1 was released in 1980 by UAS
Bangalore.
Source: Ram H., H.(2012). Crop Breeding and Biotechnology. Chapter 16,395- 407.
Exploitation of Male sterility in
Pigeon Pea
• MS system used is GMS and CMS.
CMS cytoplasm in Pegion Pea
Cytoplasm Source
A1 Cajanus scarabaeoides
A2 Cajanus sericeus
A3 Cajanus volubilis
A4 Cajanus cajanifolius
A5 Cultivated Pegion pea
Exploitation of in CMS Pigeon Pea
a) A1 Cytoplasm:
Cajanus scarabaeoides x cultivated Pigeon pea
Hybrid
Draw back: male sterile plant derived from this
cross found to have female sterility.
b) A2 cytoplasm:
Cajanus sericeus x short duration advanced
breeding line
Hybrid
Draw back:
• F1 was partially male sterile
• Backcross population were found segregating
for male sterility.
A4 Cytoplasm is a boon for hybrid
breeding in Pegion Pea.
• Closely related to cultivated type.
• No morphological defects
• Produce plenty of pollen with restorer lines in
hybrid combination.
• Stable male sterile source
• Capable of producing high yielding hybrids.
Source: Saxena, K, B. et.al(2006).. Commercial pigeon pea hybrids are just a few
steps away. Indian Journal of Pulse Research. Vol 19:1, 7-16.
• In cotton, GMS has been reported in upland, Egyptian
and arboreum cottons.
• In tetraploid cotton, male sterility is governed by both
recessive and dominant genes. However, male sterility
governed by recessive genes is used in practical plant
Breeding.
• Sixteen different genes in tetraploid cottons (13 in G.
hirsutum and 3 in G.barbadense) and two in G.
arboreum have been identified for genetic male
sterility.
Exploitation of in MS Cotton
• Sterility is conditioned by dominant alleles at five loci
viz, MS4, MS7, MS10, MS11 and MS12
• By recessive allele at other loci viz. msl, ms2, ms3,
msl3, msl4 (Dong A), msl5 (Lang A) and msl6 (81
A).
• Two male sterile phenotypes viz. m5ms6 and ms8ms9
are conditioned by duplicate recessive factors.
• The expression of male sterility varies greatly in
extent and stability among the loci.
• In diploid cotton, two genes have been identified for
GMS from Akola and HAU, Hisar.
• At Akola, the male sterility was obtained from
anomalum x arboreum crosses while at Hisar it was
identified as a spontaneous mutant in arboreum
variety DS 5.
• G. hirsutum line Gregg (MS 399) from USA is the
basic source of GMS possessing ms5ms6 gene for
male sterility.
• G. harknessii was the only available source of CMS until 1997.
• After concerted efforts, the cytoplasmic lines with G. aridum .
(D4) has been developed by the Cotton Research Unit, PDKV,
Akola.
• A new system of CMS has also been developed at the University
of Arkansas, USA wherein G. trilobum cytoplasm was utilized.
• The new system of cytoplasm called CMS 8 (D-8) has undergone
extensive testing to eliminate undesirable effects (eg. Low fibre
maturity) of the G. hirsutum nucleus interaction with the G.
trilobum cytoplasm.
• Another different source of CMS i.e CMS-C1 has been recently
developed by using G. sturtianum.
Exploitation of in CMS Cotton
Lines Genotypes
GMS LRA 5166, SRT 1, DGMS 1,
HGMS 2
CMS Rajat, Supriya, Laxmi,
Adadhita, Narmada
Restorer line NH 258, AKH 545, GSR 22,
AKH 39 R, DR 1
Source: Singh, B., S. et.al(2011)... CICR technical bulletinn:24:, 1- 15.
List of Hybrids in cotton using GMS
Role of Apomixis in cotton
• Apomixis is production of seed without fertilization
and is considered as one of the tool in plant breeding.
• So far, apomictic gene has not been reported in
cotton.
• However, use of certain chemicals like dimethyl
sulphoxide and colchicine in combination have been
effective when applied to 16 to 20 days old flower
buds.
• Occurrence of apomictic plants have also been
reported in some interspecific crosses of cotton.
• Apomixis will be of great help when the breeder
desires to fix the hybrid vigour.
• Thus, once the desirable combination has been
selected, the hybrid could be multiplied and
maintained by apomictic progeny.
• It will increase the efficiency of the hybrids by three
or two line breeding system apart from the use of
seed every year.
• The availability of large number of hybrids will help
to increase genetic diversity and reduce genetic
vulnerability.
Recent Studies
Title: New Temperature Sensitive Genic Male Sterile
Lines with Better Outcrossing Ability for Production of
Two-Line Hybrid Rice.
Authors: Arasakesary ,S.,R. et.al.
Year of Publication: 2015
Journal: Rice Science . Vol,22(1): 49-52.
Place:
• Regional Agricultural Research and Development
Center, Iranamadu Junction, Kilinochchi, Sri Lanka;
• Paddy Breeding Station,Tamil Nadu Agricultural
University, Coimbatore 641003, India.
Ppt on exploitation of male sterility in monocots and dicots
Conclusion
• TGMS line seeds are generally produced in cool season which
favours pollen fertility, as low temperature (< 23 °C) results in
fertility while high temperature (> 30 °C) results in sterility
irrespective of the photoperiod.
• This experiment revealed that all these three TGMS lines viz.
TNAU18S, TS-29-150GY and TNSU60S had considerable
outcrossing potentials with respective 39.3%, 24.6% and
17.6%, and also the cross combinations of
TNAU18S/IET21508, TNAU18S/IET21044,
TNAU18S/IET21009, TNAU60S/CB-09-106,
TNAU60S/IET21009 and TS-29-150GY/DRR3306 had
showed perfect synchronization period with acceptable hybrid
seed production which would pave the way for using TGMS
system under local condition.
Reference
• Source: Ram H., H.(2012). Crop Breeding and
Biotechnology. Chapter 2,96- 98.
• Source: Charles S, L.(1990). The Texas Cytoplasm of Maize.
Cytoplasmic Male sterility and Disease susceptibility. Science.
Vol(250), 942-947.
• Source: Saxena, K, B. et.al(2006).. Commercial pigeon pea
hybrids are just a few steps away. Indian Journal of Pulse
Research. Vol 19:1, 7-16.
• Source: Singh, B., S. et.al(2011)... CICR technical
bulletinn:24:, 1- 15.
• Source: Singh, B, D.(2012). Plant Breeding , Principles and
Methods. Chapter 22,355.
Thank You

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Ppt on exploitation of male sterility in monocots and dicots

  • 3. INTRODUCTION Male sterility is defined as an absence or non- function of pollen grain in plant or incapability of plants to produce or release functional pollen grains.
  • 4. Why to Exploit Male Sterility in Plants?? • Eliminate emasculation in hybridization. • Increase natural cross-pollination in self pollinated species. • Controlled pollination plants. • Facilitate commercial hybrid seed production.
  • 5. Exploitation of Male sterility in Rice In rice following types of male sterility systems are used  Cytoplasmic Male Sterility( Three line breeding)  Genetic Male Sterility( Two line breeding).  Male sterility induced by Chemical Hybridizing Agents.
  • 7. Cytoplasmic Male Sterility in rice • Male Sterility is governed by cytoplasmic genes or plasmagenes. • Source of Male Sterility.
  • 8. WA-CMS lines • WA-CMS lines are the most widely deployed lines in hybrid rice production . • Pollen abortion in WA-CMS occurs relatively early during microspore development, mainly at the uninucleate stage (Luo et al.2013), resulting in amorphous aborted pollen grains. • The pollen abortion is determined by the genotype of sporophytic tissues, not by the genotype of the pollen itself. • Efforts are made to transfer this WA cytosterility into genetic background of elite breeding lines.
  • 9. Treatment with 1% Pottassium iodide a) Stained pollens- viable pollen b) Unstained pollens - non viable pollen
  • 10. CMS Line Developed At Elite line converted Origin of elite line IR 46826 A IRRI IR 10154-23-3-3 IRRI IR 46827 A IRRI IR 10176-24-6-2 IRRI IR 46828 A IRRI IR 10179-2-3-1 IRRI IR 46829 A IRRI IR 19792-15-2-3-3 IRRI IR 46831 A IRRI Jikkoku Seranai 52-37 India IR 46883 A IRRI MR-365 India IR 46885 A IRRI PAU 269-1-8-4-1-1-1 India Madhu A CRRI ,India Madhu India HR 7017 A Iri Korea Samkangbyeo Korea Source: Ram H., H.(2012). Crop Breeding and Biotechnology. Chapter 2,96- 98.
  • 11. Procedure of utilization of CMS line in Seed production
  • 12. Hybrids developed using CMS in India Hybrid Parentage APRH- 1 IR 58025 A x Vajram APRH-2 IR 62829 A x MTU 9992 MGR- 1 IR 62829 A x 10198-66-2R KRH-1 IR 58025 A x IR 9761-10-IR CNRH-3 IR 62829 A x Ajaya R DRRH-1 IR 58025 A x IR 40750-82-2-2-3R KRH-2 IR 58025 A x KMR 3 Pant Shankar Dhan 1 IR 58025 A x UPRI 192-133R CORH 2 IR 58025 A x C 20 R ADTRH 1 IR 58025 A x IR66 Sahyadri IR 58025 A x BR 827-35-3-1-1-R Narendra Sankar Dhan 2 IR 58025 A x NDR 3026-3-I-R Source: Ram H., H.(2012). Crop Breeding and Biotechnology. Chapter 2,96- 98.
  • 13. Why There is Switch over from three line to two line breeding?? • Cytoplasmic male sterility- fertility restoration system based on three line breeding is often difficult to maintain. • Problems in the maintenance of A line. • Lack of diversity in A and R lines, and the presence of minor fertility genes in B lines ,lead to low heterotic potential and high seed production costs. • Hence two line breeding emerged.
  • 14. Exploitation of GMS in Rice • Male sterility is governed by nuclear genes. • In GMS ,the GMS line produce 50% of male fertile plants and 50% male sterile plants. • So its very much necessary to identify and eliminate male fertile plants before pollen shed . • Thus this draw back of GMS is overcome by using Photoperiod Sensitive GMS. Temperature sensitive GMS.
  • 15. Procedure of utilization of TGMS/PGMS line in Seed production
  • 16. Exploitation of TGMS in Rice Consider the Temperature/ Thermo Sensitive Genetic Male Sterile line 5460S. TGMS line Temperature Expression 5460S < 28 ᵒC Complete male fertility > 30ᵒC Complete male sterility Source: Singh, B, D.(2012). Plant Breeding , Principles and Methods. Chapter 22,353- 355.
  • 17. Maintenance of TGMS line in rice LOCATION A (< 28 ᵒC Temperature) TGMS line (5460S.) selfing TGMS line (Complete fertile) Source: Singh, B, D.(2012). Plant Breeding , Principles and Methods. Chapter 22,353- 355.
  • 18. Utilization of TGMS line in Hybrid Seed Production LOCATION B (> 30ᵒC Temperature) TGMS line (5460S.) x Male fertile line Hybrid Seed (Produced on TGMS line ) Source: Singh, B, D.(2012). Plant Breeding , Principles and Methods. Chapter 22,353- 355.
  • 19. List of PGMS lines in Rice Genetic Control lines Origin Expression of MS Controlled by two recessive genes PGMS lines Nongken 58S Spontaneous mutation Day length shorter than 13.75hr X88 Hybridization Day length shorter than 13hr MG 201 EMS Mutagenesis Day length 12hr Source: Singh, B, D.(2012). Plant Breeding , Principles and Methods. Chapter 6,90.
  • 20. List of TGMS lines in Rice Genetic Control lines Origin Expression of MS Controlled by single recessive gene Annong- 1s Spontaneous mutation Temperature of 27 ᵒC Hennong S Hybridization Temperature < 29ᵒC 5460S Irradiation Temperature >30ᵒC SM-38 Spontaneous mutation Temperature < 22ᵒC SM-5 Spontaneous mutation Temperature < 22ᵒC JP-2 Spontaneous mutation Temperature < 26ᵒC JP-38S Spontaneous mutation Temperature > 30ᵒC Source: Singh, B, D.(2012). Plant Breeding , Principles and Methods. Chapter 6,90.
  • 21. Hybrids developed using PGMS in Rice Hybrid Parentage Hunan Pei ai 64s x Tequing Anhui 7001s x Xiusui 04 Hubei 7001s x 1514 Guangdong Pei ai 64s x Shuanging11 Sichuan Pei ai 64s x Shuangingyou 1 Source: Ram H., H.(2012). Crop Breeding and Biotechnology. Chapter 2,100.
  • 22. Exploitation of Male Sterility by CHA’s in Rice  Application of chemicals at specific growth stage of crop results in chemically induced male sterility and chemical components used are called as Chemical Hybridizing agents.  Two arsenical CHA’s are used in Rice MG1( based on zinc methyl arsenate) MG2 (based on sodium methyl arsenate)
  • 23. CHA’s in Hybrid seed Production in Rice • Male sterility is artificially induced by spraying gametocides to cause stamen sterility without harming the pistil. • The chemical which makes sterile the stamen, plant can be used as female parent for producing hybrid seed. • Two selected lines are planted in alternate strips and one is utilized as female (chemically sterilized) and is pollinated by the other line, for production of hybrid seed.
  • 24. Rice hybrids based on CHA’s in rice • Qing – Hua- Fu- Gwi. • Gang – Hua- Qing – Lan. • Gang – Hua- 2. • You- Za-1. • Ya- You-2. • These are produced in china using arsenical CHA’s. Source: Singh, B, D.(2012). Plant Breeding , Principles and Methods. Chapter 22,355.
  • 25. Exploitation of MS in Maize CMS in Maize • Three main CMS types have been described:  CMS-T (Texas) (Rogers and Edwardson, 1952) • Fertility restoration is sporophytic • Rf1 (chr. 3) & Rf2(chr.9) are responsible for fertility restoration  CMS-C (Charrua) (Beckett, 1971) Fertility restoration is Sporophytic •Rf4, Rf5, Rf6 are responsible for fertility restoration  CMS-S (USDA) (Jones,1957) • Fertility restoration is Gametophytic • Rf3 (chr. 2) are responsible for fertility restoration
  • 27. T cytoplasm in maize • Texas (T) cytoplasmic male sterility discovered in 1940s. • used extensively throughout the 1960s. • Highly stable under all environmental conditions. • Characterized by failure of anther exertion and pollen abortion.
  • 28. • Plants bearing the T cytoplasm- susceptible to race T of the southern corn leaf blight - (Cochliobolus heterostrophus = Bipolaris maydis) • Widespread use of T-cytoplasm for hybrid corn production led to epidemic in 1970 in USA, with the widespread rise of Race T of the southern corn leaf blight . • Thus utilization of T cytoplasm as a source of Male sterility was stopped and detasseling is being used to get male sterile plants. Failure of T cytoplasm in maize Source: Charles S, L.(1990). The Texas Cytoplasm of Maize. Cytoplasmic Male sterility and Disease susceptibility. Science. Vol(250), 942-947.
  • 29. Exploitation of Male sterility in Bajra • The discovery of cytoplasmic-genic male-sterility often called cytoplasmic male-sterility and the development of male-sterile line Tift 23A1 (Burton 1958, 1965) laid the foundation of the pearl millet (Penniseturn americanum) hybrid seed industry in India. • The first commercial hybrid was HB 1. • It was bred on Tift 23A1 at the Punjab Agricultural University (PAU), Ludhiana, India, and it showed a 100% yield average over the open-pollinated check varieties (Athwal 1966).
  • 30. • The A, cytoplasm of Tift 23A1 has been extensively utilized in breeding a wide range of male-sterile lines. • At Tifton, four male-sterile lines were produced either by transferring single genes for specific traits into Tift 23A1 and Tift 23B, or by backcrossing Non restorers into Tift 23A1 cytoplasm. • Of these, mostly Tift 23A1, and to some extent Tift 23DA, were used in India for hybrid production. • Hybrids on Tift 23A1 yielded more than those on Tift 23DA1. • Five hybrids on Tift 23A1 were released in quick succession between 1965-1972, but all went out of cultivation within about 5 years of their release due to high downy mildew susceptibility.
  • 31. Source of Cytoplasmic Male sterility in Bajra Cytoplasm Source CMS line Remarks A1(Tifton) Mutation Tift 23 A, Tift 23 D2 A Used for commercial cultivation A2 (Ludhiana) Mutation L66A Incomplete expression of MS A3 P. Violaceum L67A Extremely stable for male sterility ,very promising restores in bajra A4 Half sib pool of the early gene pool ICMA-90111 Developed at ICRISAT
  • 32. Hybrids in Bajra Hybrid Parentage HB1 Tift 23 A x BIL 3B HB2 Tift 23 A x J88 HB3 Tift 23 A X J104 HB4 Tift 23 A x K 560 HB5 Tift 23 A x K559 Source: Ram H., H.(2012). Crop Breeding and Biotechnology. Chapter 6,187- 209.
  • 33. Exploitation of Male sterility in Sunflower • MS system used is Cytoplasmic Genetic Male sterility system. • Here both cytoplasmic genes and nuclear genes govern the Male sterility. • Source of male sterility in sunflower is CMS- PET1 cytoplasm. • CMS-PET1 cytoplasm of sunflower arose from an interspecific cross between Helianthus petiolaris and H. annuus.
  • 34. Utilization of CMS PET Cytoplasm in seed production
  • 37. Hybrids developed Using CGMS system in Sunflower • Armavirtsky 3497 • BSH1 • BSH2 • BSH3 PET Cytoplasm. • BSH4 (source of CMS) • BSH5 • BSH6 • BSH8. • Out of this BSH 1 and BSH2 found to be promising hybrids for seed production. • First Sunflower hybrid BSH1 was released in 1980 by UAS Bangalore. Source: Ram H., H.(2012). Crop Breeding and Biotechnology. Chapter 16,395- 407.
  • 38. Exploitation of Male sterility in Pigeon Pea • MS system used is GMS and CMS.
  • 39. CMS cytoplasm in Pegion Pea Cytoplasm Source A1 Cajanus scarabaeoides A2 Cajanus sericeus A3 Cajanus volubilis A4 Cajanus cajanifolius A5 Cultivated Pegion pea
  • 40. Exploitation of in CMS Pigeon Pea a) A1 Cytoplasm: Cajanus scarabaeoides x cultivated Pigeon pea Hybrid Draw back: male sterile plant derived from this cross found to have female sterility.
  • 41. b) A2 cytoplasm: Cajanus sericeus x short duration advanced breeding line Hybrid Draw back: • F1 was partially male sterile • Backcross population were found segregating for male sterility.
  • 42. A4 Cytoplasm is a boon for hybrid breeding in Pegion Pea. • Closely related to cultivated type. • No morphological defects • Produce plenty of pollen with restorer lines in hybrid combination. • Stable male sterile source • Capable of producing high yielding hybrids. Source: Saxena, K, B. et.al(2006).. Commercial pigeon pea hybrids are just a few steps away. Indian Journal of Pulse Research. Vol 19:1, 7-16.
  • 43. • In cotton, GMS has been reported in upland, Egyptian and arboreum cottons. • In tetraploid cotton, male sterility is governed by both recessive and dominant genes. However, male sterility governed by recessive genes is used in practical plant Breeding. • Sixteen different genes in tetraploid cottons (13 in G. hirsutum and 3 in G.barbadense) and two in G. arboreum have been identified for genetic male sterility. Exploitation of in MS Cotton
  • 44. • Sterility is conditioned by dominant alleles at five loci viz, MS4, MS7, MS10, MS11 and MS12 • By recessive allele at other loci viz. msl, ms2, ms3, msl3, msl4 (Dong A), msl5 (Lang A) and msl6 (81 A). • Two male sterile phenotypes viz. m5ms6 and ms8ms9 are conditioned by duplicate recessive factors. • The expression of male sterility varies greatly in extent and stability among the loci.
  • 45. • In diploid cotton, two genes have been identified for GMS from Akola and HAU, Hisar. • At Akola, the male sterility was obtained from anomalum x arboreum crosses while at Hisar it was identified as a spontaneous mutant in arboreum variety DS 5. • G. hirsutum line Gregg (MS 399) from USA is the basic source of GMS possessing ms5ms6 gene for male sterility.
  • 46. • G. harknessii was the only available source of CMS until 1997. • After concerted efforts, the cytoplasmic lines with G. aridum . (D4) has been developed by the Cotton Research Unit, PDKV, Akola. • A new system of CMS has also been developed at the University of Arkansas, USA wherein G. trilobum cytoplasm was utilized. • The new system of cytoplasm called CMS 8 (D-8) has undergone extensive testing to eliminate undesirable effects (eg. Low fibre maturity) of the G. hirsutum nucleus interaction with the G. trilobum cytoplasm. • Another different source of CMS i.e CMS-C1 has been recently developed by using G. sturtianum. Exploitation of in CMS Cotton
  • 47. Lines Genotypes GMS LRA 5166, SRT 1, DGMS 1, HGMS 2 CMS Rajat, Supriya, Laxmi, Adadhita, Narmada Restorer line NH 258, AKH 545, GSR 22, AKH 39 R, DR 1 Source: Singh, B., S. et.al(2011)... CICR technical bulletinn:24:, 1- 15.
  • 48. List of Hybrids in cotton using GMS
  • 49. Role of Apomixis in cotton • Apomixis is production of seed without fertilization and is considered as one of the tool in plant breeding. • So far, apomictic gene has not been reported in cotton. • However, use of certain chemicals like dimethyl sulphoxide and colchicine in combination have been effective when applied to 16 to 20 days old flower buds. • Occurrence of apomictic plants have also been reported in some interspecific crosses of cotton.
  • 50. • Apomixis will be of great help when the breeder desires to fix the hybrid vigour. • Thus, once the desirable combination has been selected, the hybrid could be multiplied and maintained by apomictic progeny. • It will increase the efficiency of the hybrids by three or two line breeding system apart from the use of seed every year. • The availability of large number of hybrids will help to increase genetic diversity and reduce genetic vulnerability.
  • 51. Recent Studies Title: New Temperature Sensitive Genic Male Sterile Lines with Better Outcrossing Ability for Production of Two-Line Hybrid Rice. Authors: Arasakesary ,S.,R. et.al. Year of Publication: 2015 Journal: Rice Science . Vol,22(1): 49-52. Place: • Regional Agricultural Research and Development Center, Iranamadu Junction, Kilinochchi, Sri Lanka; • Paddy Breeding Station,Tamil Nadu Agricultural University, Coimbatore 641003, India.
  • 53. Conclusion • TGMS line seeds are generally produced in cool season which favours pollen fertility, as low temperature (< 23 °C) results in fertility while high temperature (> 30 °C) results in sterility irrespective of the photoperiod. • This experiment revealed that all these three TGMS lines viz. TNAU18S, TS-29-150GY and TNSU60S had considerable outcrossing potentials with respective 39.3%, 24.6% and 17.6%, and also the cross combinations of TNAU18S/IET21508, TNAU18S/IET21044, TNAU18S/IET21009, TNAU60S/CB-09-106, TNAU60S/IET21009 and TS-29-150GY/DRR3306 had showed perfect synchronization period with acceptable hybrid seed production which would pave the way for using TGMS system under local condition.
  • 54. Reference • Source: Ram H., H.(2012). Crop Breeding and Biotechnology. Chapter 2,96- 98. • Source: Charles S, L.(1990). The Texas Cytoplasm of Maize. Cytoplasmic Male sterility and Disease susceptibility. Science. Vol(250), 942-947. • Source: Saxena, K, B. et.al(2006).. Commercial pigeon pea hybrids are just a few steps away. Indian Journal of Pulse Research. Vol 19:1, 7-16. • Source: Singh, B., S. et.al(2011)... CICR technical bulletinn:24:, 1- 15. • Source: Singh, B, D.(2012). Plant Breeding , Principles and Methods. Chapter 22,355.