3. INTRODUCTION
• Mitochondria are consider as the power
house of the cell because in it the energy is
liberated and released in the form of ATP
which is generated thorough the ADP+Pi.
• It contains their own DNA. Most of the
proteins of this organelle are imported to the
cytosol after their synthesis. Most of the
proteins are synthesized outside the cytosolic
ribosome.
4. • Newly formed proteins are released into the
cytosol. The receptors recognise specific
uptake targeting sequences in the new
proteins.
• Targeting of sub proteins acquires sequential
action of two targeting sequences and two
membrane bound receptor. One is to direct
the protein into the mitochondria & the other
to direct the correct organelle or membrane.
• Generally the protein uptake into the
mitochondria is an energy requiring process
that depends upon the Mito. Membranes.
5. List of some mitochondrial proteins
Location of proteins in mitochondria Name of the proteins
MATRIX 1. ALCHOL DEHYDROGENASE (YEAST)
2. CARBOXYLIC PHOSPHATE SYNTHETASE
3. CITRATE SYNTHETASE & OTHER CITRIC
ACID ENZYMES
4. F1 ATPase SUBUNIT , α,β, & γ
5. ORINTHINE METHYL TRANSFERASE
ORITHININE TRANS CARBOXYLASE
INNER MEMBRANE ADP/ATP ANTIPORTER
CYT.Q CoQH2 (C – REDUCTASE COMPLEX)
SUB UNIT 1,2,5,6,7& 8 Cyt. C OXIDASE
SUBUNIT
PHOSPHATE/OH ANTIPORTER
PROTEYOLYTIC OF F0 ATPase
6. INTER MEMBRANE SPACE Cyt. C
Cyt. C PEROXIDASE
Cyt. B2 & C1 (SUBUNIT OF CQH2- Cyt-
REDUCTASE COMPLEX)
OUTER MEMBRANE MITOCHONDRIAL PORINS (H2O
TRANSPORT ) P70
7. SYHTHESIS OF
MITOCHINDRIAL PROTEINS
• Most of the proteins of mitochondria are
synthesised in the cytosol.
• All newly formed mitochondrial proteins are
formed are initially located in the cytosol.
• Travelling proteins have their each n-terminus
residues comprises most targeting sequences.
• The receptor signal sequences are not identical
receptor itself recognises such sequences at
different destination from mitochondria now are
ale to bind through a different but related
sequences.
9. PROTEIN TARGETTING IN
SORTING
• Mainly most of the proteins of the cytosol or in the
mitochondria are targeting by the two chaperons
mitochondrial import stimulation factor (MSF)i.e. HSP-
70 and HSP-60.
• The sites from where it goes in side the matrix of the
mitochondria is two types TOM-22 & TOM-23 they get
further binds up with TOM-70 & TOM-37.
• Targeting while in sorting of proteins is done by the
two processes these are CO-translational import and
post translational import it occurs at the site where
chaperons proteins act n brings proximity an d made a
PMF across the membrane.
10.
11. CONCLUSION
• FROM THE ABOVE DISCCUSSION WE
KNOW ABOUT THE MITOCHONDRIAL
PROTEINS THEIR SYNTHEISI I N
DIFFERENT SITES INSIDE THE
ORGANELLE THEN WE KNOW ABOUT
THE WAY HOW THEY TARGETTING AT
THEIR DESIRED LOCATION/SITES IN
THE MITOCHONDRIA OR CYTOSOLAND
HOW THEY GENERATES THE ENERGY I
NTHE FORM OF PHOSPHATS.
