Instrumentation pH meter use, Application and calibration

1. F.Y.B.Sc ZOOLOGY
SEMESTER I
PAPER II: UNIT 3
INSTRUMENTATION
Presentation by : Veena

2. • MICROSCOPY
• COLORIMETRY
• pH
• CENTRIFUGE
• CHROMATOGRAPHY
• ELECTROPHORESIS
• INTRODUCTION, PRINCIPLE (how it works)
APPLICATIONS

3. pH meter

4. Potentiometric pH meter

5. Use to estimate hydrogen ion concentration
i) Understanding nature of sample (Acidic/Basic/Neutral)
ii) Knowing exact pH of sample for manipulating chemical
reactions for synthesis of certain molecules
iii)Acid based titrations (endpoint graphically determined)
iv) Pharmaceutical industry (drug needs to be of sp. pH)
v) Medical science (diagnosis burning throat/tooth decay)
vi) Dairy industry (cheese quality and other products)
vii)Agricultural (soil pH : crop cultivation, good yield)
viii)Aquaculture and
ix) water consumption

6. What is pH ? And why there was need of inventing the term
Enzymes (reactions in body)
Hydrogen ion concentration
Danish naturalist ; Soren Peter Lauritz sorenson 1909 ; pH scale

7. pH scale by
Danish biochemist : SOREN PETER LAURITZ SORENSON 1909

8. Weak acid & Strong acid
Weak base and Strong base
Buffer : A solution that resists the change in pH
Home work: examples of WA,SA, SB,SA
Examples of natural buffers
Few must know terminologies/concepts