2. WHAT ARE PCR ?
• Polymerase chain reaction ( PCR ) is a rapid and versatile in vitro
method for amplifying defined target DNA sequences present within
the source of DNA.
• This technique was formulated by Kary Mullis in1985 .
• PCR are conventional technique .
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5. WHAT ARE REAL TIME -PCR
1. RT- PCR is a method in which RNA first is converted to cDNA and
then cDNA is amplified by PCR.
2. RT-PCR is a very sensitive technique for detecting and quantifying
RNA.
3. There are four steps to RT-PCR
• cDNA synthesis by reverse transcription
• Denaturation
• Primer annealing