PCR

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PCR

  1. 1. PCR   & ITS APPLICATIONS
  2. 2.
  3. 3. Polymerase Chain Reaction <ul><li>PCR means Polymerase Chain Reaction </li></ul><ul><li>It is a technique used to amplify the DNA </li></ul><ul><li>It was developed in 1984 by Kary Mullis </li></ul><ul><li>In 1993 Mullis won the Nobel Prize </li></ul><ul><li>Thermal cycler is an apparatus used to carryout a PCR reaction </li></ul>
  4. 4. Polymerase Chain Reaction <ul><li>Minute amount of starting material </li></ul><ul><li>Quick and simple </li></ul><ul><li>Extremely sensitive </li></ul>
  5. 5. <ul><li>Components required for a PCR reaction </li></ul><ul><li>Taq DNA polymerases </li></ul><ul><li> Target sequences </li></ul><ul><li>Deoxyribonucleoside triphosphates </li></ul><ul><li>Oligomer primers </li></ul><ul><li>Amplification buffer </li></ul>Polymerase Chain Reaction
  6. 6. Procedure - PCR cycle Polymerase Chain Reaction
  7. 7. Procedure - Polymerase Chain reaction Polymerase Chain Reaction
  8. 8. Procedure - Polymerase Chain reaction Polymerase Chain Reaction Denaturation Annealing Dna synthesis
  9. 9. Procedure - Polymerase Chain reaction Polymerase Chain Reaction Denaturation Annealing Dna synthesis
  10. 10. DNA cloning for sequencing DNA-based Phylogeny Functional analysis of Genes The diagnosis of Hereditary diseases The identification of Genetic Fingerprints ( used in forensic sciences and paternity testing ) The detection and diagnosis of Infectious diseases Polymerase Chain Reaction
  11. 11. Polymerase Chain Reaction What is PCR? What is the use of PCR? Protocol Applications
  12. 12. Polymerase chain reaction Polymerase Chain Reaction
  13. 13. Polymerase Chain Reaction
  14. 14. Temperature profile of a typical PCR cycle Polymerase Chain Reaction
  15. 15. Polymerase Chain Reaction
  16. 16. DNA cloning for sequencing DNA-based Phylogeny Functional analysis of Genes The diagnosis of Hereditary diseases The identification of Genetic Fingerprints ( used in forensic sciences and paternity testing ) The detection and diagnosis of Infectious diseases 06/06/09 Polymerase Chain Reaction
  17. 17. Temperature profile of a typical PCR cycle Polymerase Chain Reaction

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