screening of physicochemical and phytochemical properties

1. Swapnil Prashant Gautam
M.Tech Food Technology
28 NOV, 2016
Gautam Buddha University Greater Noida Uttar Pradesh
EVALUATION OF NUTRITIONAL COMPOSITION OF
TINOSPORA CARDIFOLIA STEMS FOR THE DEVELOPMENT
OF VALUE ADDED FOOD PRODUCTS

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Scientific name: Tinospora cordifolia family
Menispermaceae.
The plant has its origin in India but now in abundantly in
tropical Areas of india,shri lanka,and Myanmar.
it mostly grow on neem or mango trees.
In recent year increasing chikungunya or dengue or plain
fever in india. Giloy is the superfood you should have every
single day to treatment diseases.
The stem of Giloy is of maximum utility,but root can also be
used. Its benefits and have even been approved by the
FDA(Food and Drug Administration),Giloy is a universal
herbs herb that help boost immunity.
INTRODUCTION:

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According to WHO about 390 million cases of dengue
fever worldwide and 96 million are require medical the
treatment.
According to National Vector Borne Disease Control
Programme (NVBDCP),around 18000 cases of dengue in
Delhi.
Indian also saw doubling up cases of fever 2015 to 2016.
Giloy is a universal herbs herb that help boost immunity
and resolved the problem of fever.
Introduction

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It has anti-tumor, anti-arthritic, antioxidant, anti-bacterial and
anti-Malerial properties.
It aids in digestion and is also well-known as a highly effective
blood purifier.
It is used to treat many ailments such as cardiac debility, gout,
anemia, leprosy, jaundice, as well as other serious diseases like
cancer.
 In recent times, Giloy has become hugely popular due to its
ability to cure swine flu, chikungunia or dengue.
Enhances the functioning of protective cells, macrophages, and
improves body's resistance to infections.
Applications

5. Properties
Antidiabetic
Immunity
booster
Antipyretic
Antiallergic
anticancer
Anti-inflammatory
antiaging
Blood
purifier
Application Tinospora cordifolia

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Procedure For Value Added Food Product
Screening of
herbs
Proximate
composition
Hordeum
vulgare Tinospora
cordifolia
Fresh barley
removes
impurity and
dry
Grinding
Filter by
120mm sieve
Barley
powder
prepared
Cut into small
size Removed
impurity
Dry for 8 to 9
hrs at 60 °C
Granding
Barley podwer
desovled into
water for 24 hrs
Collect
barley water
Filter by 120
mm sieve
Giloy powder
Giloy mixed in
barley water
400mg/kg

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Raw materials: The stem part of Tinospora cordifolia was
collected from Greater Noida Gautam Buddhnagar area in
Uttar Pradesh India.
Chemical Used: Acetic acid, H2SO4, lead acetate,
distrilled water.
The proximate composition of Tinospora cordifolia was
determine by using the below described methods.
1. Physicochemical properties
2. Phytochemical properties.
Material And Methods

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Moisture content : Moisture content is an indicator amount
of water present in food samples.
Formula:
Procedure:
Physico-chemical Properties
5g sample
Hot air oven at
105c for 3 hrs
Take ready after
1hrs using
degiccator
Take the final ready
using degiccator

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Total Ash Content
Wiegh
accurate 2g
powder of
Giloy
Heating on
hot plate to
removes fat
or oils
Put in the
muffle
furnace for 3
Hrs cooled in
Degicator

10. Detection Of Tannins:
50 mg powder + 5ml D
water
Added 3ml of 10% lead
acetate
A bulky white precipitation
occurs present of tannin

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Sterol Test
2mg powder in 1ml
acetic acid
Heating to boiling and
cooled
Add 1 ml of H2So4
Upper layer gives dark green
color present of sterol

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FLAVONOID TEST
Few drop of 10%
lead acetate +1 ml of
test solution
Yellow color
precipitate conferm
the flavonoid

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SAPPONIN TEST
5 ml of test solution
Shake well for 5 mint
Formation of stable
foam conferm
saponnin

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Carbohydrate Test
2mg powder +1ml D water
1ml of fehming solution(A+B)
Shake and heat on water both for
10 mint
Brick red color precipitate conferm
the carbohydrate

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Result and discussion
Preperation of barley Water:
 Preparation of barley water ratio is 80:10:24, 80ml water, 10 ml sample, 24
hrs soaking time. after soaking collect the fresh water and removed waste
sample.
Preperation of Stem Tinospora cordifolia Value added food Product:
 Development of value added food product by using of barley water with
stem powder tinospora cordifolia carefully using the dose response of giloy
podwer.
 Making 3 sample 40ml, 80ml, 120ml added podwer .10g, .2g, .4g well mixed
for 10 mintutes. After that adding the preservation in the sample according to
food safety standardrization and authority of India.
 Sodium benzoate is used as preservative of their samples.very small amount
of preservative used in goloy juice

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Show the Physico-chemical properties of T. cordifolia Result 1
68.5%15.9%
11.4%
4.2%
1. Loss of drying
2. Total ash content
3. Moisture content
4. Remaining Material

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Show Phytochemical Analysis of Stem T.cordifolia
Result 2
Series 1
Sterol Flavonoid Carbohydrate Sapponin Tannin
3.2
0 0 0
8
SERIES 1
Series 1

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40
0.1 0.0012
80
0.2 0.0025
120
0.4 0.005
0
20
40
60
80
100
120
140
Water Sample Preservative
Chart Title
Series 1 Series 2 Series 3
Graphical Representation Of Value Added Food Result 3

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Incorporation of fresh and dehydrated stem mixture of
barley water and Giloy will enhance the nutritive value of
traditional recipes by improving their micronutrient
content.
Incorporation of dehydrated stem mixture powder in
various recepies can be helpful for therapeutic purposes
and can be included in the diets of people with various
diseases like anti-inflammatory, diarrhea, dysentery,
Immune enhanced, antioxidant. it is recommended to be
included in the diet of all age groups because of its healthy
benefits.
Conclusion

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THANK YOU