Antibacterial Activity of Stem Bark Extracts of Oroxylum indicum an Endangere...
1st project
1. Medicinal Properties And Insecticidal
Properties Of (Wild Watermelon)
Citrullus colocynths (Linn.) Schrad.
2. IT is genarally found in farms as a weed
means there is no use of this climber.
3. Their different part perform different
medicinal properties as follow
1. Seed
2. Fruit and Fruit Pulp
3. Leaves
4. Root
4. SEED
The Seeds are rich in fatty acids such as
myristic, palmitic, stearic, oleic, linoleic and
linolenic acids.
Citrullus oil has a good potential use as cooking
oil.
The Investigation revealed that seed extract
have an insulin tropic effect which show anti
diabetic activities.
5. FRUIT AND FRUIT PULP
The fruit is pungent, a cooling purgative,
antihelmetic, antipyretic and carminative.
The fruit pulp is purgative, diuretic and is
used against gonorrhea.
A fruit of Citrullus colocynthis is traditionally
used for the treatment of diabetes, microbial
diseases, ulcer, inflammation, jaundice and
urinary diseases in Asian and African
countries.
6. LEAVES
The leaves exhibit anti-inflammatory activity
and are diuretic.
8. INSECTICIDAL PROPERTIES
Cucurbitacin E Glycoside Isolated from Citrullus
colocynthis show insecticidal effect against
Aphis craccivora.
Aphis craccivora can spread viruses.
After NMR and IR spectrophotometer analysis
we confirmed that the compound which isolated
from Citrullus colocynthis and has insecticidal
effect against Aphis craccivora was 2-O-β-D-
glucopyranosylcucurbitacin E. (Torkey.H.M
and Abou-Yousef,H.M.,2009)
9. ANTIMICROBIAL ACTIVITY
METHODS -
In this procedure we can use either 1st or 2nd
methods to examine antimicrobial activity
AGAR- WELL DIFFUSION METHOD.
DISC DIFFUSION METHOD.
10. AGAR- WELL DIFFUSION METHOD
Petriplates -- 20ml medium -- 24hr culture of
bacterial strains.
Wells -- 20 µl of the plant extracts added.
Incubated at 37°C for 24 hours.
Measure diameter of obtained antimicrobial
activity.
11. DIAGRAM - AGAR- WELL DIFFUSION METHOD
Plant
extract Bacteria
growing on
medium.
Zone of
inhibition
12. DISC DIFFUSION METHOD
Prepare agar plates
Inoculation of test microorganisms with spread
plate method
Filter paper discs -- 6mm in diameter -- soaked
with 15µl of the plant extract
Place (and press) in agar plate but no closer
than 24 mm from each other, center to center
Incubate -- 37ºC -- 16 to 18 hours.
Measure diameter of obtained antimicrobial
activity.
13. DIAGRAM – DISK DIFFUSION METHOD
Filter paper
disk with
plant extract.
Bacteria
growing on
medium.
Zone of
inhibition
14. PROCEDURE
Collection and identification of plant
material
Collect fresh climbing plant.
Confermed by botany teacher.
Expose to air till it completely dried.
Preparation of extract
Leaf(Citrullus colocynthis) – Dried –
Powdered – extract by soxhlate (aqueous) –
concentrated by Rota evaporator – Extract
15. Leaf(Citrullus colocynthis) – Dried –
Powdered – extract by socslate (Ethanol) –
concentrated by rota evaporator – Extract
pripared.
Seed = Dried–powdered- extract by socslate
(aqueous) – concentrated by rota evaporator
– Extract pripared.
Root = Dried–powdered- extract by socslate
(aqueous) – concentrated by rota evaporator
– Extract pripared.
17. Material -
15 Petri plates – Conical flask containing
nutrient agar – 15 suspension tubes containing
saline were sterilized for further use.
Bacterial Strains –
E.coli
Bacillus megaterium
Bacillus cereus
Pseudomonas aeruginosa
Streptococcus mutans
18. Actual Method –
Pour agar (20ml) in each Petri plate
Bacterial strains were poured and spread
Make well (about 6mm) in Petri plate at
center
Fill the well with respective extract.
Finally incubate the plates for 24 - 48 hrs
19. OBSERVATIONS
After incubation we get a zone of inhibition
around these well.
The zone of inhibition is as follow
Leaves -
Bacterial Strains Zone of inhibition
Aqueous
E.coli Ethanol
Bacillus megaterium
Bacillus cereus
Pseudomonas aeruginosa
Streptococcus mutans
20. OBSERVATIONS
Seed and Root –
Bacterial Strains Zone of inhibition
Seed Root
E.coli
Bacillus megaterium
Bacillus cereus
Pseudomonas aeruginosa
Streptococcus mutans
21. RESULT –
The bitter water melon (Citrullus colocynthis)
has antimicrobial (bacteria) activity.
CONCLUSION -
There is a great promise for development of
novel drugs from the plant Citrullus
colocynthis for dreadful human diseases.
They affects the growth of insect and
degrade them so it can use in several
insecticide, pesticide etc.
22. They have some medicinal properties i.e
antidiabetic activity,
They shows the antibacterial activity which
helps to inhibit the growth of bacteria which
affects the plants.
