Preliminary phytochemical screening and antimicrobial activity of Ulva sp.

1. PRELIMINARY PHYTOCHEMICAL SCREENING AND
ANTIBACTERIAL ACTIVITY OF ULVA FASCIATA
ISOLATED FROM COASTAL WATER OF BAY OF
BENGAL NEAR VISAKHAPATNAM, ANDHRA
PRADESH.
Submitted by-
Sarif mahammad
4th Semester
Department of Botany
University Of Kalyani

2. SEAWEEDS OR MARINE ALGAE ARE PRIMITIVE NONFLOWERING PLANTS
WITHOUT TRUE ROOT STEM AND LEAVES. THEY ARE THE RENEWABLE LIVING
SOURCES OF FOOD, FEED, AND FERTILIZER IN MANY PARTS OF THE WORLD.
THEY HAVE BEEN SCREENED EXTENSIVELY TO ISOLATE LIFE-SAVING DRUGS OR
BIOLOGICALLY ACTIVE SUBSTANCES WORLDWIDE.
DIFFERENT PARTS OF THE THALLI ARE ALSO KNOWN TO DIFFER IN THEIR
ANTIMICROBIAL POTENTIAL.
THE MARINE ALGAE FOR EXAMPLE ULVA FASCIATA, ALSO KNOWN AS LIME
PALAHALAHA AND SEA LETTUCE IS COMMON GREEN ALGA THAT IS USED FOR
CONSUMPTION IN MANY PARTS OF THE WORLD.
THESE SEAWEEDS ARE IMMENSE PHARMACEUTICAL AND AGRICULTURAL VALUE
. MANY COMPOUNDS ARE ISOLATED FROM GREEN ALGAE ARE KNOWN TO
EXHIBIT BIOLOGICAL ACTIVITIES .
ULVA SPECIES ARE RICH IN ESSENTIAL NUTRIENTS AND THEY EXHIBIT ANTI-
PEROXIDATIVE AND ANTI-HYPERLIPIDAEMIC ACTIVITIES.

3. Materials and Methods
Study Area:The marine alga was collected from the sea coast of
Visakhapatnam ,Andhra Pradesh,India.
Collection Of Sample: Samples are used as the experimental algae to study
their bioactivity.Samples were rinsed with sterile water to remove any associated
debris.Sample was kept under sunshade.After drying the sample it was ground
to the powder form.The podwer was thenused for the estimation of
phytochemical compounds and antimicrobial activity.
Study Programme: Study conducted in the months of February 2020. Various
physicochemical & biological parameters uses for analysis.:
:

4. RESULT:
Table-1: Phytochemical screening of Ulva fasciata
PHYTOCONSTITUENT PRESENCE or ABSENCE
Carbohydrates +
Tannins +
FLavonoids
(aglycone)
++
Flavonoids
(glycosides)
+
Saponins -
Terpenoids +++
Alkaloids ++
Anthraquinones
(aglycones)
-
Cardiac glycosides -
+++ present in higher amounts, ++ present in moderate amounts, + present in lower amounts.
Table-2: Antibacterial activity of crude extract of U. fasciata against Gram positive bacteria.
EXTRACT Streptococcus
faecalis
Bacillus
subtilis
Bacillus
cereus
Staphylococcus
aureus
Water 18 20 21 18
Methanol 20 22 24 21
benzene 16 16 15 12
Table-3: : Antibacterial activity of crude extract of U. fasciata against Gram negative bacteria.
EXTRACT Pseudomonas
aeruginosa
E. coli Serratia
marcescens
Shizella
dysenteri
Shizella
flexneri
WATER 16 18 16 16 15
METHANOL 18 20 18 20 18
BENZENE 13 14 12 17 16

5. :Important Findings:
The present study suggest that marine green alga U.fasciata have a
more potential values of preliminary phytochemicals.
Screening of this plant extract revealed the presence of all the
phytochemicals tested except saponin,anthraquinone(aglycones) and
cardiac glycoside.
This plant showed large scale antibacterial activity and can be used to
discover bioactive natural products that may serve as leads for the
development of new pharmaceuticals.
Terpenoids present in higher amounts,alkaloids and flavonoids present
in moderate amounts.

6. FUTURE PROSPECT
Marine sources are receiving much attention mainly because of the contents
of functional ingredients such as polyunsaturated acids, β-carotene and their
pigment carotenoids, sulfated polysaccharide, and sterol.
These seaweeds are easily available
The extracted phytochemicals are not artificial so the demand of people is
increasing.
As infectious diseases are a major cause of morbidity and mortality
worldwide, systematic exploration of these marine seaweeds would give
valuable antimicrobial lead compounds and drugs which can be exploited
commercially as pharmaceutical industries also recognize the compounds
derived from natural resources.

7. CONCLUSION
 Ulva fasciata has great potential as antimicrobial compounds against
clinical pathogens.
 The result revealed that Gram positive bacterial cells are more susceptible
than the Gram negative ones
 This plant showed large scale antibacterial activity and can be used to
discover bioactive natural products that may serve as leads for the
development of new pharmaceuticals that address higher to unmet
therapeutic needs.
 To strengthen the global scientific effort, in the present study the
phytoconstituents found in the Ulva fasciata are documented.

8. THANK YOU