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Production Of
Alcohol By
Fermentation
BY- SANCHIT DHANKHAR
Alcohol
•Any series of hydroxyl comppounds derived
from saturated hydrocarbons.
•It is aclear liquid that has a strong smell.
•A colourless, volatile, flameable liquid
which is produced by fermentatiopn.
Test Organism
 The test organism used here is known as
Saccharomyces cervisae.
 It is a type of yeast which is used in the production of
alcohol.
 It produces the enzyme invertase in both a
cytoplasmic and a secreted form.
 This yeast ferments a number of common substrates
including sucrose and maltose.
Starter
 The starter is prepared from the selected yeast
isolated in pure culture.
 A tube contaioning 10ml of of sterile wort is
inoculated from pure culture of yeast.
 After incubation for 25-30C for suitable time,culture
in tube may be used to inoculate 200ml of sterile
mash.
 Aeration is useful in the preparation of starter.
Formation of Alcohol
Sugar Crops Starches
Lignocellulos
e
Extraction of
juice
Enzyme
Hydrolysis
Steam
Explosion
Fermentation
Recovery
Distillation Dehydration
Alcohol
Formation of Ethyl Alcohol:
Ethyl alcohol is prepared with the help of
molasses
Molasses +
Water
Mixer
Mash +Yeast
Fermenter
Beer Column
Anhydrous
Alcohol
Anhydrous
column
Alcohol
Refining
Column
Temperature – About 70C-80C
Time – 50 hours
 The 90-95% alcohol is further purified, dehydrated or denatured to obtain
different alcohols
Above 80C
alcohol
evaporates.
THANKYOU

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Alcohol fermentation- Microbiology

  • 2. Alcohol •Any series of hydroxyl comppounds derived from saturated hydrocarbons. •It is aclear liquid that has a strong smell. •A colourless, volatile, flameable liquid which is produced by fermentatiopn.
  • 3. Test Organism  The test organism used here is known as Saccharomyces cervisae.  It is a type of yeast which is used in the production of alcohol.  It produces the enzyme invertase in both a cytoplasmic and a secreted form.  This yeast ferments a number of common substrates including sucrose and maltose.
  • 4. Starter  The starter is prepared from the selected yeast isolated in pure culture.  A tube contaioning 10ml of of sterile wort is inoculated from pure culture of yeast.  After incubation for 25-30C for suitable time,culture in tube may be used to inoculate 200ml of sterile mash.  Aeration is useful in the preparation of starter.
  • 5. Formation of Alcohol Sugar Crops Starches Lignocellulos e Extraction of juice Enzyme Hydrolysis Steam Explosion Fermentation Recovery Distillation Dehydration Alcohol
  • 6. Formation of Ethyl Alcohol: Ethyl alcohol is prepared with the help of molasses Molasses + Water Mixer Mash +Yeast Fermenter Beer Column Anhydrous Alcohol Anhydrous column Alcohol Refining Column
  • 7. Temperature – About 70C-80C Time – 50 hours  The 90-95% alcohol is further purified, dehydrated or denatured to obtain different alcohols Above 80C alcohol evaporates.