ETHANOL PRODUCTION BY MICROBES

1. PRODUCTION
OF ETHANOL BY
MICROBES
Presented by: Joydeep pal
Student code: BWU/BBT/18/036
REG. NO: 18013000519 of 2018-19
Roll no: 18010310021
Subject: BioprocessTechnology

2. What is meant
by ETHANOL?
 Ethanol is a type of alcohol produced by
fermentation of sugars and starches or cellulosic
biomass.
 It is a colorless, flammable, clean-burning, high
octane fuel that is produced from renewable sources.
 Primary feedstock: Corn
 Secondary feedstock: sugarcane, sugar beet,
cellulose.

3. RAW
MATERIALS
 SUGARS: Sugarcane (molasses and juice), cane-sugar (clarified
concentrated syrup), sugar beet, beet sugar (diffusion juice and
beet molasses).
 Starchy materials: Corn, wheat, cassava, sweet sorghum.
 Lignocellulosic materials: Corn stover, cereal straws, sugarcane
bagasse.

4. MICRO-
ORGANISMS
INVOLVED
 Bacteria used:
❑Zymomonas mobilis
❑Clostridium acetobutylicum
❑E.coli.
• Yeast used:
❖ Saccharomyces cerevisiae
❖ Saccharomyces uvarum
❖Candida utilis

5. Features of
Microbes
 Due to the small size, having a high
surface ratio.
 Due to having a resistant cell wall,
producing high concentration substances
leads to a faster fermentation rate.
 Have the ability to “predigest” the
available food source and release both
products and the intermediate
metabolites.
 Using immobilized cells of microbes by
treating with Calcium alginate to
adsorbed on the surface of materials.
 Using genetically modified microbes to
enhance the fermentation process.
Bacteria transformed
with pGLO under
ambient light.
Bacteria transformed
with pGLO visualised
under UV light.

6. Physical and
Chemical
requirements
of microbes
oPhysical requirements:
➢The ideal pH is around 4.0-4.5.
➢The initial temperature is kept between 21-26 ºC.
➢ Ethanol gets evaporated at 27 ºC.
➢ Aeration is initially required for the growth of microbes.
oChemical requirements:
▪ Nitrogen source: Urea, ammonium sulfate etc..
▪ Phosphorus source: Diammonium phosphate is the source.
▪ Hydrolytic enzymes: They can also be added to convert biopolymers
and non-fermentable substances in the molasses to
monosaccharides or amino acids.

7. PRODUCTION
PROCESS

8. Ethanol
production by
FERMENTATION
PROCESS
 Ethanol fermentation is a biological
process in which one sugars molecule such
as glucose, fructose, and sucrose are
converted into cellular energy and thereby
produces two ethanol molecules and two
carbon dioxide molecules as metabolic
waste product.
 Yeast (Saccharomyces cerevisiae) is most
commonly used to perform this
conversion in anaerobic condition.
 Zymomonas mobilis is frequenly employed
for alcohol production.

9. Ethanol
Utilization
 The personal care products industry is one of the largest users
of industrial ethanol.
 Ethanol is also used in many deodorants, lotions, hand
sanitizers, soaps, and shampoos.
 100% ethanol is not used generally as motor fuel. A percentage
of ethanol is combined with the unleaded gasoline.This is
beneficial because the ethanol:-
➢Decreases the fuel cost
➢Increases the fuel octane rating
➢Decreases the gasoline’s harmful emissions.

10. THANKYOU  I would like to thank our respected HOD sir and also
thankful to Uma sir.