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ALCOHOL FERMENTATION       PREPARED BY:PRACHI       PATEL       SEM:III       SAURASHTRA UNIVERSITY       RAJKOT
INDEX: INTRODUCTION PROCESS ORGANISMS USED SUBSTRET AND MEDIA  COMPOSITION AND IDEAL  CONDITION PRODUCT RECOVERY USE...
alcoholic fermentation, also  referred to as, Ethanol  fermentation, is a biological  process in which sugars such as  glu...
Conversation Mechanism            C6H12O6            C2H5OH + CO2              180                92       88            1...
C6H12O6    ====> 2(CH3CH2OH)        +    2(CO2) +       Energy                                              (which is stor...
Organisms used: Bacteria:   Zymomonas mobilis   Closteridium acetobutylicum   Klebsiella pneumoniae Yeast   Saccharo...
FERMANTABLE SUBSTRATE   Sugary materials   Starchy material   Cellulosic materials
SUGARY MATERIALS:MolassesSuger caneSugar beetSweet potatoSulfide wasteWeet sorgumWheyglucoseSuccroseLactose
STARCHY MATERIALScereals: wheat,maize,barley,sorgum,corn,riceRoots:     potato,tropicaMild products:      wheat flour,corn...
CELLULOSIC MATERIALSWoodPaper wasteAgricultural waste
Fermentation mediaNutrient   Raw materialCarbon     molasses, starchNitrogen   corn steep liquor, soybean           meal, ...
CONDITIONS FOR FERMENTATIONCarbon sources: pure sugar or crudesugars/molasses (10-18%).Nitrogen sources: Mostly available ...
CONTN…….. Time: Depends on yeast strain.        Usual time is between 30 to 72hrs. Yield: 0.4 gallon of ethyl alcohol per ...
FERMENTATION PROCESS CARRY  OUT BY: batch fermentation Continuous fermentation    continuous fermentation is used  becau...
FERMENTATION Inoculums size:         In range of 3% to 10% with an  evrage about 4% Media:         10 -18%. Concentratio...
PRODUCT RECOVERY Distillation is a separation process for a  mixture of liquids or oils. It relies on  differences in the...
BY-PRODUCTS  Three byproducts generated1. Carbon dioxide- used for production of   dry ice and pure form used for   prepa...
Ethanol ToleranceGrowth Tolerance           Production Tolerance    Ethanol level                Ethanol level      at whi...
USES: Fuels  Some alcohols, mainly ethanol  and methanol can be used as an alcohol  fuel Preservative Solvents Alcohol...
 Alcoholic beverages Antifreeze Antiseptics Ethanol can be used as  an antiseptic to disinfect the skin.
CONCLUSION  Thus alcohol production is an   important fermentation because of   following reasons:1. Gives ethyl alcohol ...
Alcohol fermentation
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Alcohol fermentation

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its all about alcohol production.

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Alcohol fermentation

  1. 1. ALCOHOL FERMENTATION PREPARED BY:PRACHI PATEL SEM:III SAURASHTRA UNIVERSITY RAJKOT
  2. 2. INDEX: INTRODUCTION PROCESS ORGANISMS USED SUBSTRET AND MEDIA COMPOSITION AND IDEAL CONDITION PRODUCT RECOVERY USES CONCLUSION
  3. 3. alcoholic fermentation, also referred to as, Ethanol fermentation, is a biological process in which sugars such as glucose, fructose, and sucrose are converted into cellular energy and thereby produce ethanol and carbon dioxide as metabolic waste products. Because yeasts perform this conversion in the absence of oxygen ethanol fermentation is classified as anaerobic.
  4. 4. Conversation Mechanism C6H12O6 C2H5OH + CO2 180 92 88 100 Kg 51.1 Kg 48.9 KgTheoretical Yield : 51.1% Gay-Lussac Coefficient(1815)
  5. 5. C6H12O6 ====> 2(CH3CH2OH) + 2(CO2) + Energy (which is stored inATP) Sugar ====> Alcohol + Carbon dioxide gas + Energy(Glucose) (Ethyl alcohol)
  6. 6. Organisms used: Bacteria: Zymomonas mobilis Closteridium acetobutylicum Klebsiella pneumoniae Yeast Saccharomyces cerevisiae Saccharomyces carlsbergenesiae Saccharomyces saki Saccharomyces oviformis Candida utilis Mucur sp.
  7. 7. FERMANTABLE SUBSTRATE Sugary materials Starchy material Cellulosic materials
  8. 8. SUGARY MATERIALS:MolassesSuger caneSugar beetSweet potatoSulfide wasteWeet sorgumWheyglucoseSuccroseLactose
  9. 9. STARCHY MATERIALScereals: wheat,maize,barley,sorgum,corn,riceRoots: potato,tropicaMild products: wheat flour,corn feed
  10. 10. CELLULOSIC MATERIALSWoodPaper wasteAgricultural waste
  11. 11. Fermentation mediaNutrient Raw materialCarbon molasses, starchNitrogen corn steep liquor, soybean meal, pure ammonia or ammonium salts, urea, nitrate salts, phosphate saltsVitamins biotin, yeast extract, beefand growth extract, corn steep liquor,factors wheat germ meal
  12. 12. CONDITIONS FOR FERMENTATIONCarbon sources: pure sugar or crudesugars/molasses (10-18%).Nitrogen sources: Mostly available inthe form of ammonium sulphate.Growth factors: can be provided in theform of molasses.pH: 4.8-5.0.Temperature: 70-80 F. Temp. can becontrolled by cooling jacket.
  13. 13. CONTN…….. Time: Depends on yeast strain. Usual time is between 30 to 72hrs. Yield: 0.4 gallon of ethyl alcohol per one gallon of molasses. 90% carbohydrates can be converted in to alcohol.
  14. 14. FERMENTATION PROCESS CARRY OUT BY: batch fermentation Continuous fermentation continuous fermentation is used because of several advantages.
  15. 15. FERMENTATION Inoculums size: In range of 3% to 10% with an evrage about 4% Media: 10 -18%. Concentrations greater than 20% are not employed as they could be detrimental to yeast.
  16. 16. PRODUCT RECOVERY Distillation is a separation process for a mixture of liquids or oils. It relies on differences in the boiling points of the component liquids to be separated. Alcohol can be obtained by distillation and column is known as rectified column. Can also be recovered by fractional distillation. Distillate contains 95.6% ethyl alcohol and 4.4% water.
  17. 17. BY-PRODUCTS Three byproducts generated1. Carbon dioxide- used for production of dry ice and pure form used for preparation of soft drinks.2. Yeast biomass- used for animal fodder because it contains high protein, vitamins etc.3. Distillery effluents- used as a fertilizer and animal feed.
  18. 18. Ethanol ToleranceGrowth Tolerance Production Tolerance Ethanol level Ethanol level at which at which Growth ceases Ethanol production ceases 6-9% wt/vol 15% wt/vol or higher
  19. 19. USES: Fuels Some alcohols, mainly ethanol and methanol can be used as an alcohol fuel Preservative Solvents Alcohols have applications in industry and science as reagents or solvents ethanol can be used as a solvent in medical drugs, perfumes and vegetable essences such as vanilla
  20. 20.  Alcoholic beverages Antifreeze Antiseptics Ethanol can be used as an antiseptic to disinfect the skin.
  21. 21. CONCLUSION Thus alcohol production is an important fermentation because of following reasons:1. Gives ethyl alcohol used for consumption as well as industrial purposes.2. Gives byproducts like carbon dioxide, yeast biomass, fertilizer etc.3. Gives effective utilization of agro- waste eg. Molasses, sulphite waste liquor etc.

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