August 31 - 0130 - Gary Feyerseisen
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This study evaluated whether bioaugmentation or biostimulation could help meet water quality goals in woodchip bioreactors. For bioaugmentation, two bacterial strains were inoculated into woodchip bioreactors. The cold-adapted cellulose-degrading strain increased over time but the other strain's abundance dropped after 6 weeks. For biostimulation, acetate solution was added but only provided modest nitrate removal increases. The results suggest that bioaugmentation and biostimulation may help water quality goals if inoculation techniques and microbial community maintenance are improved, and if clogging and carbon delivery schemes are better managed.
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August 31 - 0130 - Gary Feyerseisen
- 1. Can bioaugmentation or biostimulation meet WQ goals? Gary Feyereisen1 Hao Wang2 Ping Wang2 Emily Anderson2 Jeonghwan Jang3 Ehsan Ghane4 Jeffrey Coulter2 Carl Rosen2 Michael Sadowsky2 Satoshi Ishii2 1 USDA-ARS, St. Paul, MN 2 U. of MN, St. Paul, MN 3 Jeonbuk National U., Rep. of Korea 4 MI State U., East Lansing, MI Acetate Sol’n
- 2. BioStim BioAug Control Nitrate-N Removal Rate ----------------------- (g N m-3 d-1) ----------------------- 15.0 5.8 4.4 BioStim BioAug Control Nitrate-N Removal Rate ----------------------- (g N m-3 d-1) ----------------------- 5.6 3.9 4.1
- 3. Campaign Date (mgC/L) (Out – In) Spr ‘17 11 May 11.0 6.3 A 18 May 3.9 -0.6 24 May 5.8 0.5 31 May 5.4 0.1 7 Jun 5.4 1 15 Jun 5.9 2.9 21 Jun 6.4 -0.2 29 Jun 6.7 -0.3 7 Jul 6.2 0.4 Fall ‘17 7 Nov 5.3 0.3 A 20 Nov 5.7 0.4 A 20 Nov 5.6 0.5 A 4 Dec 5.2 0.4 A Spr ‘18 7 May 5.2 0.2 5 June 6.2 1.3 A 30 June 7.5 0.5 A Spr ‘17 Spr ‘18
- 4. Cellulomonas sp. strain WB94 Microvirgula aerodenitrificans strain BE2.4 Campaign d after inoc. BioAug BioStim Control BioAug BioStim Control -------------- Strain detection in Woodchip Samples (% of collected samples) -------------- Spr ‘17 7 0 0 0 n/a n/a n/a Fall ‘17 14 n/a n/a n/a 63 0 25 28 n/a n/a n/a 25 13 13 42 n/a n/a n/a 38 25 13 Spr ’18 0 75 75 63 n/a n/a n/a 14 n/a n/a n/a 50 50 25 21* 75 71 25 75 25 38 WB94: Cold-adapted and cellulose-degrading bacterium increased in abundance over time. BE2.4: Initially increased in abundance, but abundance dropped to background after 42d.
- 5. Can bioaugmentation or biostimulation meet WQ goals? Acetate Sol’n Maybe, if… • Better inoculation technique • Community maintenance Yes, when… • Manage bioclogging • Better C delivery scheme DOI: 10.1002/essoar.10512241.1.
- 6. Acknowledgments Funding is gratefully acknowledged • U of MN, MnDRIVE • MN Ag. Wat. Res. Ctr. / Discovery Farms • MN Dept. Ag. • U of MN, BioTech. Inst. • U of MN, Soil Water and Climate • USDA-ARS Kim Gorans, Ed Dorsey (Star right), Scott Schumacher (Star left), and an international cast of researchers, postdocs, technicians, and students
- 7. Thank you! Q’s & Discussion DOI: 10.1002/essoar.10512241.1.
- 8. The best is yet to be discovered. ?
Editor's Notes
- USGS – in-kind support??