Anesthesia for laboratory animal

1. CPCSEA guidelines for laboratory
animal facility : Anesthesia
Presented by : Rushikesh Tidake
M.Pharm Sem III
Pharmacology
RSCP , Buldana

2. Contents
• Introduction
• Need of anesthesia
• Types of anestheisa

3. CPCSEA
Committee for the purpose of Control and
Supervision of Experimental Animals
Statutory body formed by Act of Indian
Parliament under the prevention of Cruelty to
Animals Act , 1960
Reviewed in 1998 , under the committed
chairpersonship of Meneka Gandhi
Head quarters at Chennai

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• National level expert committee to scrutinize
and approve the research projects conducted
using animals
• Introduced a National “ Good Laboratory
Practices” document
• Introduced the 3R’s for Replacement ,
Reduction and Refinement
•

5. Goals
The goal of these guidelines is to promote the human care of
animal used in biomedical and behavioural research and testing
To avoid unnecessary pain before , during and after
experiments
To provide guideline for :
• Housing
•Care
•Breeding and Maintenance
•Source of experimental animals
•Acceptable experimental procedures for anesthesia and
euthansia .

6. Functions
• Approval of Animal house Facility on the basis
of inspection conducted by CPCSEA
• Recommendation for import of animals used in
experiments
• Actions against in case of violation any legal
norm .
• Permission for conducting experiments

7. Anesthesia
• Greek word
an = without
asthesia = sensation
• It is the practice of administering medications
that block the feeling of pain and other
sensations that produce deep state of
uncounsciousness that temporarily eliminate
sensation & motor activity.

8. Need of anesthesia
• Ethical reason : for making the experiment or
procedure to be painless.
• Practical reason : as “ chemical restraint ” for
conducting the experiment or procedure
smoothly and to avoid stress & trauma to the
animal .

9. Pre anesthetic treatments
 Fasted for 8 hours
 All animals should have access to drinking
water until 1 hour prior to induction of
anesthesia
 Animals should be weighed to ensure accurate
drug dosage calculations

10. Anesthetic agents
• Ideal anesthetic agents
• Should produce reversible & controlled loss of
consciouness
• Should not disturb the physiological functions
• Causing relief from pain , stress & discomfort
• No mortality
• Easy to administered
• Should be economical

11. Types of anesthesia
• General anesthetics
• Inhalational agents : Isoflurane , sevoflurane ,
N2O
• Advantage :
• Rapid induction , recovery & precise dose
titration
• Also useful for euthansia
• Rapid elimination of drug through lungs

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• Disadvantages :
• High cost & problem of using precision
vaporizers
• Potential occupational exposure concern and
the risk of fatal over dosage
• No residual analgesic activity
• Promotes progressive depression & hpoxia

13. • Injectable anesthetic agents :
o Barbiturates : Thiopentone , Phenobarbitone
o Dissociatives : Ketamine
o Other : Urethane , Chloralose , Paraldehyde
,Magnesium Sulphate

14. • Advantages :
Do not require vapourizer & ventilation
Require a low dose for induction
Control of cardiopulmonary function is more
adequate
• Disadvantages :
• Require repeated dosing
• Unpredictable depth of anesthesia
• Some agents may cause hypotension ,
hypertension