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• CPCSEA is a statutory Committee,
• established under Chapter 4, Section 15(1) of the
Prevention of Cruelty to Animals Act 1960.
• India is one of the pioneering countries to institute
Prevention of Cruelty to Animals Act in 1960.
• Detailed rules for experimentation on animals were first
enacted by the Ministry of Agriculture in 1968.
• Present Committee was constituted on 30th August,
• At present, Shri Hem Pande, Additional Secretary,
MoEF&CC is the Chairman of the Committee.
To promote the humane care of animals used in
biomedical and behavioral research and testing.
to provide specifications that will enhance animal well
To provide quality in gaining advanced biological
knowledge that is relevant to humans and animals
Adequate veterinary care must be provided and is
the responsibility of a veterinarian.
All animals must be acquired lawfully as per the
A health surveillance program for incoming animals
should be carried out to assess animal quality.
Inspect for compliance with procurement
An effective quarantine minimizes the chance for introduction
of pathogens into an established colony.
A minimum duration of quarantine
- Small lab animals - 1 week and
- Larger animals - 6 weeks
• STABILIZATION AND SEPARATION
Newly received animals should be given a period for
physiologic, psychologic and nutritional stabilization before
Duration for stabilization will depend on the type of animal,
transportation and intended use.
Physical separation of animals by species is recommended.
• SURVEILLANCE, DIAGNOSIS, TREATMENT AND
CONTROL OF DISEASE
Observe for signs of illness, injury, or abnormal
Unexpected deaths and signs of illness should be
If animals are known to be exposed to an infectious
agent the group should be kept intact and isolated
during the process of diagnosis, treatment, and control.
Diagnostic clinical laboratory may be made available.
• ANIMAL CARE AND TECHNICAL PERSONNEL
Employ people trained in laboratory animal science.
They should be provide for both formal and on-the-job
• PERSONAL HYGIENE
It is essential to maintain a high standard of personal
Decontaminate clothing exposed to potentially hazardous
microbial agents or toxic substances.
Use disposable gear.
No permission to eat, drink, smoke or apply cosmetics in
• ANIMAL EXPERIMENTATION INVOLVING
Institutional Biosafety Committee.
The procedures must be reviewed by both the Institutional
Biosafety committee and Institutional Animal Ethics
• MULTIPLE SURGICAL PROCEDURES ON SINGLE
Multiple surgical procedures not to be practiced unless
specified in a protocol only approved by the IAEC.
• DURATIONS OF EXPERIMENTS
No animal should be used for experimentation for more than
3 years unless adequate justification is provided.
• PHYSICAL RESTRAINT
Brief physical restraint can be accomplished manually or with
Prolonged restraint of any animal should be avoided unless
essential to research objectives.
Less restrictive systems, such as the tether system or the pole and
collar system should be used when compatible with research
• The following are important guidelines for the use of restraint
Not be used simply as a convenience in handling or managing
Should be given training to adapt to the equipment.
Observe the animal at appropriate intervals.
Veterinary care should be provided if lesions or illness associated
with restraint are observed.
• PHYSICAL PLANT
The physical condition and design of animal facility hould be
well planned and properly maintained.
• PHYSICAL RELATIONSHIP OF ANIMAL
FACILITIES TO LABORATORY
Isolated far away from human habitation.
Place animal housing areas adjacent to or near laboratories
• FUNCTIONAL AREAS
Sufficient animal area required to: Ensure separation of
species or isolation of individual projects when necessary;
Receive, quarantine, and isolate animals;
Provide for animal housing.
• PHYSICAL FACILITIES
(2)Animal room doors
(a) TEMPERATURE AND HUMIDITY CONTROL
18 to 29°C temperature range3
0% to70% relative humidity
(c) POWER AND LIGHTING
A time-controlled lighting system.
Emergency power should be available in the event of power
(d) NOISE CONTROL
• ANIMAL HUSBANDRY
• (a) CAGING OR HOUSING SYSTEM:
Provide adequate space
Escape proof enclosure that confines animal safety.
Easy access to food and water.
Provide adequate ventilation.
Meet the biological needs of the animals.
Keep the animals dry and clean.
Animals should be fed palatable, non-contaminated, and
nutritionally adequate food daily.
• DESIRABLE CRITERIA
Free of toxic chemicals
Disposable by incineration
Continuous access of fresh, potable, uncontaminated drinking
Periodic monitoring of microbial contamination.
• SANITATION and CLEANLINESS
Cleaning with detergents and disinfectants.
Wire – bottom rodent cages should be washed at least every 2
Disinfection of cages by rinsing at a temperature of 82.20C (180 F).
• WASTE DISPOSAL AND PEST CONTROL
Remove regularly and frequently.
Animal tissues, carcasses, and hazardous wastes should be lined
with leak – proof, disposable liners.
EMERGENCY, WEEKEND AND HOLIDAY CARE
Animals should be cared by qualified personnel every day, including
weekends and holidays.
• RECORD KEEPING
The Animal House should maintain following records:
• Animal House plans
• Animal House staff record
• All SOPs for animals
• Breeding, stock, purchase and sales records
• Minutes of institutional Animals Ethics Committee meetings
• Records of experiments
• Death Record
• Clinical record
• Water analysis report
• STANDARD OPERATING PROCEDURES (SOPs)
The Institute shall maintain SOPs describing procedures / methods of
• Animal Husbandry
• Animal house microbial analysis
• Experimentation records.
• TRANSPORT OF LABORATORY ANIMALS
• The main considerations for transport of animals are:
• Mode of transport
• Animal density in cages
• Food and water during transit
• Protection from transit infections
• Injuries and stress
(a) Registration of establishments for Breeding of animals:
(b) Registration of establishments for experiments on animals:
(c) Approval of Animal House Facilities:
(d) Permission of Committee for Conducting Experiments:
(e) Recommendation for import
• Regulatory Mechanism :
Committee for the Purpose of Control and Supervision of Experiments on
• To ensure that animals are not subjected to unnecessary pain or suffering
before, during or after performance of experiments on them.
Sub-Committees of the CPCSEA:
1) Sub-Committee on Large Animals (SCLA).
2) Sub-Committee for selection of CPCSEA Nominees on Institutional Animals