This document discusses the production of nucleus seed for potato through tuber indexing and maintenance of virus tested stocks in 3 stages. Tubers are tested for common potato viruses like X, Y, A, and leaf diseases. Disease-free clones are selected and their progeny are planted in separate plots and bulked in stage III to produce final virus-free nucleus stocks for breeder seed production.

1. Sub.:- Principles of Seed Technology
Course No. :- APB- 5221
Credit hours:- 3(1+2)
Lecture Topic :- Nucleus seed production of Potato.
Presented by:-
Indira Gandhi Krishi Vishwavidyalaya
Raipur, C.G.
Presented by:-
Lt. Roshan Parihar, Asstt. Professor (GPB)
Deptt. of Genetics & Plant Breeding
BTC College of Agriculture & Research Station
,Sarkanda, Bilaspur,(CG)-495001

2. Nucleus seed production
of Potato

3. Potato virus X

4. Potato virus S

5. Detection methods
• Leaf sap samples of test
plants are taken and
treated with the antiserum
of X and S on a glass slide.of X and S on a glass slide.
• If virus is present
chloroplast of leaf sap
agglutination
reaction shows.

6. Potato virus Y

7. Potato virus Y

8. Potato virus A

9. Detection methods
• A6 test is carried out.
• Interspecific sp Solanum
demissum X Solanum
tuberosum var. aquilatuberosum var. aquila
leaves are detached and
kept in box containing
moist mass.

10. • Detached leaves are dusted
with 500 mesh carbondrum
powder and few drops of
phosphatic buffer solution.
• Test plant sap dropped over
the leaf and residual
carbondrum powder is rinsed
the leaf and residual
carbondrum powder is rinsed
with water.
• Leaf kept as such for 7-8
days for symptom
development.

11. Leaf roll virus of potato

12. Leaf roll of potato

13. Detection methods
• Phluroglucinol test is carried out.
• Stem portion from the base portion
taken and then transverse cutting
done.
• Portion is kept into phloroglucinol• Portion is kept into phloroglucinol
solution with 10 -11 N HCL
• After one minute portions are
removed and observed ,if found
orange red stain on primary phloem
confirms the disease.

14. Witchs Broom of potato

15. Marginal Flavescence of potato

16. Marginal flavescence

17. Potato top roll disease

18. Method of Nucleus seed
productioN
• Tuber indexing: It is the
indexing for the diseases
on the tubers taken from
single hill unit of a varietysingle hill unit of a variety
or clone.
• On a Nucleus Seed Station
four tubers of a clone
tested under glass house.

19. • Four tubers of a clone are
numbered first and tested
under glass house
conditions.
• One eye of a tuber is
scooped out and planted onscooped out and planted on
a pot and counter part is
stored. And tested for
disease using the methods

20. • Maintainence of virus
tested stocks.
• Virus tested stocks are
maintained on three stages
A. Stage –I ––A. Stage –I
B. Stage –II
C. Stage –III
––

21. • Stage –I :
• Counterparts stored tubers
are planted in field at low
aphid period
• At hills planted over 2400 M
above sea level.––
above sea level.
• Planted 1X1 m distance to
provide isolation.
• Hills are checked for
disease.
––

22. Stage –II (Progeny plots)
• Stage I clones whosoever
are found healthy are
selected and their whole
produce are planted on a––produce are planted on a
separate plot.
• Diseased reported plots
are rouged out.
––

23. • Stage –III : (Bulking)
• Clone selected under
stage II are planted in
bulk.
• Further tested for disease––
• Further tested for disease
• The final produce is virus
free Nucleus Stocks and
further used for Breeder
seed production.
––