Amino Acid production

1. Welcome To Our Presentation.
Presentation Topic
Amino Acid Production
Prepared by: Group-5
Md Rayhan ID-192046031
Abu Riad ID:192047032
Md Rakibul Hasan ID-192050031
Atiq Asif Shaown ID-192052031
Amirun Akter Zahan ID-192053031

2. What is Amino acid?
• Amino acids are the building blocks of
proteins.
• They are the group of organic compounds
containing two functional groups- amino and
carboxyl.
• The amino group (-NH2) is basic while the
carboxyl group (-COOH) is acidic in nature.
• And a side-chain (R group) specific to each
amino acid is also present.

3. Classification:
• All the 20 amino acids are not needed to be
taken in the diet.
• Based on the nutritional requirements amino
acids can be classified into two classes: -
• Essential and - Nonessential

4. Essential amino acids
• The amino acids which cannot be synthesized
by the body and need to be supplied through
the diet are called essential amino acids.
• The ten amino acids listed below are essential
for humans:
• Arginine, Valine, Histidine, lsoleucine, Leucine,
Lysine, Methionine, Phenylalanine, Threonine,
Tryptophan.

5. Non essential amino acids
• They are synthesized by the body, and do not
need not be supplied in the diet.
• These are- glycine, alanine, serine, cysteine,
aspartate, asparagnie, glutamate, glutamine,
tyrosine and proline.
• Some microorganisms are capable of
producing certain amino acids such as lysine,
glutamic acid and tryptophan.

6. Industrial Production of Amino Acids

7. Figure:
Large number of microorganisms were isolated from nature
which are capable of producing amino acids by fermentation
in commercially feasible quantities, especially from the
auxotrophic bacteria

9. • The details of production of different amino
acids by fermentation involving different
microorganisms are precised in this Table..

10. Industrial Production of Amino Acids

11. Industrial Production of Amino Acids
• Two amino acids which are produced at large
scale includes-
L-lysine
L-glutamic acid

12. Lysine Production
• lysine is the amino acid that covers more than 90% of
total world amino acid production.
• Synthesis of lysine –
80% by Fermentation.
20% by chemical synthesis.
• Fermentation Process - Submerged Fermentation
Aerobic Fermentation
• Mode of Operation - Batch Process
Fed-Batch Process
• .Fermenter Type - Stirred Tank Reactors
Air Lift Bioreactors

13. Lysine Production
• L-lysine, 2, 6-diaminohexanoic acid, is synthesized
by microorganisms either via diaminopimelic acid
pathway or the aminoadipic acid pathway.
However, in any single organism only one of the
two alternative pathways is employed. Bacteria,
actinomycetes, cyanobacteria (Blue-green algae),
some phycomycetes and protozoa use the DAP
(Diaminopimelic acid) pathway while some
phycomycetes, all ascomycetes and
basidiomycetes and eukaryotic algae uses the
aminoadipic acid pathway.

14. E.Coli Auxotroph

15. L-lysine

16. L-glutamic acid production
• Corynebacterium glutamicum, is a short, aerobic, Gram-positive rod
capable of growing on a simple mineral salt medium with glucose,
provided that biotin is also added.
• Production of L-glutamic acid by C. glutamicum is maximal at a
critical biotin concentration of 0.5 mg g-1 of dry cells, which is
suboptimal for growth
• Detergents like Tween-40,
• addition of penicillin,
• use of Glucose,
• Glucose-6P,
• CO2,
• fatty acid auxotrophic strains, or
• addition of ethambutol- inhibiting arabinogalactan synthesis.

17. L-Glutamin Acid production
Flow Chart

18. Thank You
All…