3. Content
Introduction of PCR
Principle of PCR
Instrumentation of PCR
Conclusion
Forensic Significance
Refrances
4. INTRODUCTION TO
PCR TECHNIQUE
PCR- Polymerase chain reaction
PCR is technique a used in molecular
biology to amplify a single copy or a
few copies of a segment of DNA
across several orders of magnitude,
generating thousands to millions of
copies of a particular DNA sequence.
PCR is a technique that takes specific
sequence of DNA of small amount
and amplifies it to be used for further
testing.
5. Principle of PCR
To amplify a lot of double-
stranded DNA molecules
fragments) with same
(identical) size and
sequence benzymatic
method and cycling
condition.
The DNA amplification is
detected in real-time with
the help of a fluorescent
11. Conclusion
PCR is not only vital in
the clinical laboratory
by amplifying small
amounts of DNA for
STD detection .
The PCR technology
can also be employed
in law enforcement,
genetic testing of
animal stocks and
vegetable hybrids, and
drug screening along
12. Forensic Significance
of PCR
Polymerase chain reaction (PCR) is currently
the method of choice for the identification of
human remains in forensic coursework.
DNA samples from crime scenes often contain
co-purified impurities which inhibit PCR.
PCR can be used as a tool in genetic
fingerprinting.
it possible to develop forensic genetics tools
that allow both very informative routine
investigations and still more and more
advanced, special investigations in cases
concerning crime, paternity, relationship,