VIDEO WITH EXPLANATION IN THE LINK BELOW 👇👇 https://www.youtube.com/watch?v=s1dF0BuW64E&t=9s 👇👇👇 (Reference Books 📚 Available below) 👇👇👇 References📚 🔑 PASSWORD - RAMEG123 📚 Clinical Biochemistry An Illustrated Colour Text, 5e by Allan Gaw https://tii.la/l8zwft4u297 📚 Clinical Chemistry Principles Techniques Correlations by Michael Bishop https://tii.la/h7aqB52kT 📚 Naik Essentials of Biochemistry https://tii.la/Q65 📚 Satyanarayana-biochemistry https://tii.la/038zfK7dd5v 📚 Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostic https://tii.la/wJRgNhF

2. DEFINITION
It's the movement of charged particles in an electrolyte
when subjected to an electric field
Uses (Separation of)
Serum
Urine
CSF
Biological Fluids

3. PRINCIPLE
Velocity of movement of particles in an electric field which
depends on :-
The net charge of molecule, strength of electric field
It is inversely proportional to molecular weight

4. THEORY
Movement of charged molecules in a medium subjected to an
electric field
v = Eq / f — Equation 1
v = Velocity of molecule
E = Electric field (V)
q = net charge of molecule
F = Frictional coefficient dependent on mass and shape of molecule

5. THEORY
Electrophoretic mobility (µ): rate of migration per unit field strength
µ = v / E — Equation 2
Further derived as
µ = Eq / Ef — Equation 3
µ = q / f

6. FACTORS AFFECTING ELECTROPHORESIS
Net electric charge of the molecule
Size and shape of molecule
Electric field strength
Properties of the supporting medium
The pH of medium
Temperature of operation
Wick flow

7. CLASSIFICATION
Electrophoresis
Technique Position Dimension
Zone Moving Horizontal Vertical 1D 2D 3D
Boundary
Paper
Gel
Agarose
PAGE
Capillary

8. ZONE ELECTROPHORESIS
Technique produces zones which can be observed in a
separated manner.
Classification
Paper Electrophoresis
Gel Electrophoresis
Agarose Electrophoresis
PAGE

9. MOVING BOUNDARY ELECTROPHORESIS
Technique that's carried out in solution, without a
supporting medium
Classification
Capillary Electrophoresis

10. POSITIONAL ELECTROPHORESIS
2 Types
Horizontal
Vertical

11. 1D ELECTROPHORESIS
• Separation of proteins based on molecular weight over range-10 to
300kDa
• Samples weighed and dissolved in Sodium Dodecyl Sulfate (SDS)

12. 2D ELECTROPHORESIS
Essential tool for separation and fractionation of complex protein
mixtures from biological samples
Depend on
• Isoelectric point (pI)
• Isoelectric focusing (IEF)

13. 3D ELECTROPHORESIS
Protein analysis in a separation medium that extends substantially in 3
spatial dimensions
Analyzed in 3 independent separation parameters:
Native size
Isoelectric Point
Molecular weight
cc