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ELECTROPHORESIS
AND
ITS PRINCIPLE
BY- SHRISTI SAINI
What is electrophoresis?
• Electro refers to the energy of electricity and phoresis, means to carry
across.
• Electrophoresis is a technique for separating charged molecule in a
mixture under the influence of an applied electric field.
PRINCIPAL
• Electrophoresis generally follows two laws:-
1-Law of electrostatic
2-Law of frictional force
• Any charged molecule or ion migrates when placed in an electric field. The rate of migration
of a compound depends on its net charge, size, shape, and applied current.
V=Eq/f
V=velocity of migration of charge
E=Electric field in volt
q=net electric charge on molecule
f=frictional coefficient which function as
Mass and shape of molecule.
PRINCIPAL
• The movement of charged molecule in an electric field is often
expressed in term of electrophoretic mobility, which is defined as
“the velocity per unit of electric field”
µ=v/E=Eq/fE=q/E
µ=electrophoretic mobility
Thank you.

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ELECTROPHORESIS-mobile.pptx

  • 2. What is electrophoresis? • Electro refers to the energy of electricity and phoresis, means to carry across. • Electrophoresis is a technique for separating charged molecule in a mixture under the influence of an applied electric field.
  • 3. PRINCIPAL • Electrophoresis generally follows two laws:- 1-Law of electrostatic 2-Law of frictional force • Any charged molecule or ion migrates when placed in an electric field. The rate of migration of a compound depends on its net charge, size, shape, and applied current. V=Eq/f V=velocity of migration of charge E=Electric field in volt q=net electric charge on molecule f=frictional coefficient which function as Mass and shape of molecule.
  • 4. PRINCIPAL • The movement of charged molecule in an electric field is often expressed in term of electrophoretic mobility, which is defined as “the velocity per unit of electric field” µ=v/E=Eq/fE=q/E µ=electrophoretic mobility