Micropropagation of banana with respect to nonconventional and tissue culture techniques my research

1. MICROPROPAGATION OF BANANA PLANT
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MICRO PROPAGATION OF BANANA WITH
RESPECT TO NATURAL AND TISSUE
CULTURE TECHNIQUES.
ABSTRUCT:
The object of this paper is to determined the mythology differences by
particularly applying series of two techniques for the propagation of
banana plant with respect to conventional and tissue culture techniques so
being the appropriate technique for their propagation they taken with
series of experiments to be applied. By the use of different chemicals and
processes the technique gave different factors of growth the experiments
taken on banana plant.
Keywords: NATURAL TECHNIQUE, MICROPROPAGATION
TECHNIQUE, SUCKER (EXPLANT).
NTRODUCTION:
Plant tissue cultureis culturing of any part of plant specificallyunder
some sterile or aseptic conditions so as laboratory methods are
used. the methods taken so far depends on the plant sample to be
propagated as naturally or at laboratory level, with specified type of salts
and chemicals taken in consent to be used regarding the methods.
I

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BANANA:
Banana is a common sort of plant for herbaceous plant as a fruited
producing plant. hay are particularly native of south and south Asia they
are abundantly cultivated as a fruited barring souse throughout the globe
,their fruit barring dependent on species that are of two with respect to
their cultivation. They are energy boosters for infants .bananas produced
from tissue culture are somehow free from diseases. Bananas are referred
as ‘POOR MAN’S APPLE’
Bananas are ranked fourth next to wheat maize. For the present bananas
are grown in around 150 countries around the globe.
Banana culturing has the potential of being rapid growth and as well as it
has the abilitytobe changed in length also in taste by performing different
techniques in regards to obtain good to best quality this is how it is being
called the most effective way of obtaining desirable forms. Somehow some
specific sort of chemicals and sterilents are useable to get through specific
ideas of improving the quality to its peak.
Properties:
 There areabout 110 caloriesin a banana
 Safeand pure enough for baby'sfirst solid food
 Bananasare a terrific, heart-healthyfood
 A nutrient dense food that'sidealfor any diet or weight-lossprogram
 Fat-, cholesterol- and sodium-free
 Providesan excellent sourceof vitaminB6.
METARIALS AND METHODS:

3. MICROPROPAGATION OF BANANA PLANT
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Typicallyall these research hasbeen taken out through thefacilitated
materialare present at web that has been accumulated through different
sites and article,keeping in focus on the two cultivating techniquesof
propagationsuch as naturaland tissue culturetechnique that hasto be
differentiated accordingtotheir accuracyand usageor rapidness.The
techniquesare liberated separately.
MICROPROPAGATION:
[1]Productionof a specified or desired plants of largenumber of plantsin
focused withina short spanof duration, with low cost of work.[1]
Thismethod carries thoroughlytheculturemedia withinthetwo to three
different stagesunder some specific conditions oflaboratory.
[1] Micropropagating thebanana shoot tip is the main method for banana
plants. , once a plantlet come out of shell or developed through the tissue-
culture technique,[1] recklessness should be avoided
Thoroughly moving it from its sterile, artificially controlled
Environment to the more exposed greenhouse and less
Protected field conditions. Counts all in..
MICRPROPAGATION TECHNIQUES:
Some sort of successful micropropagation strategies are occurred as a
output of plant material,
Logically a plant Tissue Culture facilityhasto be comprises some sort of
separateroomsfor media,
MATERIALS:
Preparation, aseptictransfer Sterrilents, stocks, air flow hood system,
greenhouse, gelling supporters, cultureincubation and illuminated rack
systems.
An aseptic condition constitutes.
Preparation steps carry out through series of steps, as..

4. MICROPROPAGATION OF BANANA PLANT
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Preparation got through with the, [2] sword suckers maincomponent of
propagationof banana along with. Media preparationarea thatso far
comprises of a largetableto keep in media safe thoroughly, such as
cooking utensils like ,wise,[2]
Stainless steel vessels along with a tap to dispense media in glass
containers.
[1] (PICTURE SOURCE) S L V Plant Technologies Pvt. Ltd [1]
PROCEDURE:
THE PROCEDURE GETS STARTED BY THE SELRCTION OF MOTHER PLANT.
THE IGNITION OVER OF MICROPROPAGATION HAS DEPENDENCY ON THE
WELL SELECTION OF SUCKERS THAT ARE SO OBTAIN ABLE FROM THE
MOTHER PLANT, IN CASE TO GIVE RISE TO THE NEW MICROPROPAGATED
PLANT WITHTHE HELP OF EXPLANTS PRODUCTION BY TISSUE CULTURE
METHODS SO AS INCONNECTION WITH SELECTION MOTHER PLANT MUCT
HAVE THE FOLOWING PROPERTIES.. AS DITTO.

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 [4] ‘’Desire propagatingplant hasto be well healthy, of true to type
and must be free from diseases and viruses and pests as well,’’[4]
 ‘’The flowers budshave to be retainablein case of checking of the
presenceof pests.’’
 ‘’Mother plants should be present awayof other banana plantswith
distanceof approximately500 m to gainpurityand to get avoid
spread of virus’’
Weedy diseases’’
 [4] ‘’plant has to be propagated verymaximum management
provisio, so as maintainsofar the true expression’’[4]
 [4] ‘’Explants Hasto be selected thoroughlyas that a ,Mother plant
should be healthy, true to type and out any kind of diseasesand
pests, especiallyvirus diseases.’’
The process taken out through some series of stages..
All of processing materials are first sterilized through some sort of
chemicals or starilents that can lead them to b perfect sterilized,
If any sort of glass wear is used so far they should be sterilized with alcohol
that helps to eradicate all sort of microbes form glass wears.
Autoclave is the best of all process used for sterilizing the samples,
Banana got through propagationastheyare cultivated on both ways sexual
as well as asexual ways.
So far Seed propagation is common in wild species which
Are diploid and undergo normal meiosis, as fertilization factor.

6. MICROPROPAGATION OF BANANA PLANT
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Propagation of banana takes out through the suckers such as.
In mean while the multiplication of suckers (EXPLANT) is common way
that is practiced byfarmers.that some,how is a cheapest of methods but in
sameway it comprises some sort of disadvantages as suckers are typically
not true type of their mother plants and are usually exposed to pests and
vulnerable disease so to avid this all tissue cultureis the technique that can
overcome of all these vulnerabilities this method involves the culturing of
pseudo stem tissue of micro propagated plants of banana plant in aseptic
conditions.
STEPS:
 First take out the sample and start soaking it in a mixture that is
contained with chemicals such as 20 % Clorox and with valuable
drops of tween 20 for at least 20 to 30 minutes.
 Use starillants such as specific chemicals or distilled water to rinse
the pseudstem foe couple of minutes.
 For now take out the rest of leaf sheath until and unless the
merristem approaches or seen, in length approximately 2.5 to 3.5 in
tall.
 So for now transfer the pesudstem with pealed sheath consisting of
meristem toarea of propagation or culture media to specified media
of propagation.
 Banana with its initial growth takes place so they to be taken to
medium of initiation of nutrient skooge media .that has been filled
with required nutrients for its initial growth.

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 These steps leads to initial growth of banana so far infect with time
this new plant has to be take in care. And has to be subculture
periodically with 7 to 8 times.
 When the initiation got through the new plant has to be transfer to
the green house for special care using some sort of gelling agents
for support as a solid support, that might be any agent so far gas
solids or filters some, how some sort of growth regulators can also b
use for boosting of growth, the hardening has two rational ways such
as Primary and Secondary hardening that are depending on their
sterilents being used for their hardening process.
[1] (PICTURE SOURCE) S L V Plant Technologies Pvt. Ltd [1]
ADVANTAGES:

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 [3]’’Higher rates of multiplication occur through this method for
healthy plants’’ [3].
 ‘’Requireminimum rangeof spacefor a large number of plant banana
propagation thoroughly.’’
 ‘’Planting of banana occurs with highly healthy banana production
as they are not so exposed to the disease and pests with perfect
quality.’’
 [3’’Low cost and all of it with lowest time of cultivation with
maximum yield.[3]’
ECONOMICAL IMPORTENCE:
Culturing through plant tissue of banana has the potential to give rise to
some superior qualitybananasso as to get boost export throughout the year
for the best of production yearly this method has effects over the economy
by producing moreand more verities to be exported round the globe has it
has the ability to get into job with conversely so low time and high quality
over time, with cheap and good quality the product has some allure effects
on population to relay over this methods that can be available throughout
the year not on to annual dependent, potentially it has great effects on
economy of country economy as to provide more and more quality
products available at each period of year.
NATURAL PROCESS:
[2]Bananas require at least of specified temperature round about 13c to
38c along with RH regime 75% to 85%.[2] Bananas are propagated
vegetative, rather than sexually infect, nearly all of its varieties are
seedless and are cultivated parthenocarpically in environment, the
propagation is done through the division of sucker or as pulp that used to
arise from the main stem or as from underground corm, usually verities
differs from the usage of farmers behalf as they use or go for specified

9. MICROPROPAGATION OF BANANA PLANT
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verities as they desire for also with different sizes by the choice of suckers,
the cultivation isdone in worm months, at the specific stagesof young plant
carehas to be taken thoroughly go for a large scale of cultivations, at that
casepulp has to be leave with light by cutting off extra leaves from the pulp
the probably help the plantain to grow vigorously in the field. Quality of
cultivationdependson the type of sample, soil type, as well as the nutrient
being used for the best cultivation of banana at the field.
RESULTS AND DISCUSSIONS:
So far for the propagation of specific plant that has the ability of
propagation through natural process as well as it has the advantage of
cultivationthrough a conventionalprocessso named as micro propagation.
The method are carried through series of process and procedures as this
paper is note been tested through experimentsbut has taken combinations
of different research papersthat areexperienced with practical tests as well
as affiliated scholarsand published thoroughlyin generals of world renown
publications. Bananas at all most consuming fruit of the world so natural
process to some extentshas thelimited or limitationusein some corners of
the globe but tissue culturing has provided a vast way of facilitating the
word with the samestint fruit at its best by doing so the tissue culturing of
banana using itssuckers that arebeing so called the propagate able parts of
the plantainof banana it has so need real care for the propagation of plant
weather it is conventional or non conventional process.
CONCLUTION:
It is so concluded that banana can be grown in vitro as well as in vivo in
case to get some potential, banana are so far most consuming product
through the globe so its importance has given rise to the alternative
methodsof propagationthat isso called micropropagationmethod thegive
bolster to the banana production topeak of its best qualityround the world
and will available round the year to all country or even non producing
countries as well just by using the conventional method of propagation.

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