NEUBAUER CHAMBER

1. CELL COUNTING
DR.MANJULA MBBS MD (PATHOLOGY)

2. IMPROVED NEUBAUER CHAMBER
RULED CHAMBER AREA
RAISED EDGES

3. IMPROVED
NEUBAUER CHAMBER

4. RULED CHAMBER AREA = 3 X 3 mm
9
large
squares,
each
measuring
1
x
1
mm
2
WBC are counted in 4 corner squares, each corner
square has 16 small square, each measuring 1/16 mm2
RBC
are
counted
in
central
square
which
has
25
small
squares,
each
measuring
1/25
mm
2

5. WBC COUNTING TECHNIQUE
• Volume of one large square = 1 X 1 X 1/10 mm3
• Volume of four large square = 1 X 1 X 1/10 X 4 mm3
= 2/5 mm3
• Assume that WBC in four large square as N
• 2/5 mm3 of blood contain, WBC = N X dilution factor
• 1 mm3 of blood contain, WBC = N X 50

6. RBC COUNTING TECHNIQUE
• Volume of one small square = 1/5 X 1/5 X 1/10 mm3
• Volume of five small square = 1/5 X 1/5 X 1/10 X 5 mm3
= 1/50 mm3
• Assume that RBC in 25 small square as N
• 1/50 mm3 of blood contain, WBC = N X dilution factor
• 1 mm3 of blood contain, RBC = N X 10.000