- A peripheral smear (PS) provides important diagnostic information about red blood cells, white blood cells, platelets, and any hemoparasites present.
- Romanowsky stains like Leishman and Giemsa stains are commonly used because they differentially stain cellular components through a combination of basic and acidic dyes.
- To perform a peripheral smear, a small blood sample is spread in a thin layer on a slide and allowed to dry before being stained using a Romanowsky stain and examined under a microscope.
This presentation is about introduction to 'PATHOLOGY' and the very Basic process of 'TISSUE PROCESSING' along with Introduction to the Body Cells, Epithelium and Tissues.... It will be helpful to 2nd year MBBS students for better understanding of the subject.. Thank You..!!
This presentation is about introduction to 'PATHOLOGY' and the very Basic process of 'TISSUE PROCESSING' along with Introduction to the Body Cells, Epithelium and Tissues.... It will be helpful to 2nd year MBBS students for better understanding of the subject.. Thank You..!!
These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
MANAGEMENT OF ATRIOVENTRICULAR CONDUCTION BLOCK.pdfJim Jacob Roy
Cardiac conduction defects can occur due to various causes.
Atrioventricular conduction blocks ( AV blocks ) are classified into 3 types.
This document describes the acute management of AV block.
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
2. PS most important, valuable, frequently asked Ix in Hemat
Labs.
It provides information on:
RBC morphology:- Diagnose anemia.
WBC disorders and DLC.
Platelet number and morphology:- Diagnose bleeding disorder.
Cross check CBC parameters.
Detect hemoparasites.
INTRODUCTION
3. Blood cells contain cellular structures with different reaction (pH), some
are acidic while others are basic.
Aniline dyes are of two types:
Basic dye – Methylene blue.
Acidic dye – Eosin.
All stains with combination of acidic and basic dye are called
ROMANOWSKY STAINS.
STAINS
4. First prep by ROMANOWSKY in 1891.
Methylene blue on oxidation produces coloured compounds – AZURES
have ability to combine with EOSIN.
Oxidation – maturation / chemical treatment.
Azures - different shades of staining the smears.
RBC – pink.
Granules of Eosinophils – orange.
Granules of Basophil – bluish black.
Granules of neutrophils – lilac.
Nuclei and structures – stained by basic dye – basophilic.
Nuclei and structures – stained by acidic dye – eosinophilic.
ROMANOWSKY
STAINS
5. Dissolve 1 g of Leishman powder in 500ml of acetone free methyl alcohol
(fixative) “{acetone destroys cells}” in conical flask.
Warm it at 50 c – 15 min.
Ripening – sunlight exposure – 2 days.
LEISHMAN STAIN
6. Dissolve 1 g of Giemsa stain powder in 66 ml of glycerol at 60 C for 2 hrs.
After cooling add 66ml of acetone free methyl alcohol – mix.
37 C – one week – ripening.
OTHER STAINS:
WRIGHT STAIN.
JENNER STAIN.
JENNER – GIEMSA STAIN.
FIELD STAIN.
GIEMSA STAIN
7. Drop of blood – clean grease free glass slide – 1 cm away from one end.
Place smooth edge of spreader over blood drop and spread the blood along
its edge.
Place slide at 30-40 degree angle – make smear – smooth forward
movement.
Ideal smear – 2.5-3.5cm – length.
Dry at room temperature.
Label.
PERIPHERAL SMEAR
8.
9. Length : 2/3 to 3/4 of slide length.
Shape : tongue.
Head, body, tail.
SOURCES OF ERROR:
Grease – blank oval areas.
Spreader edge not smooth – tail of smear ragged (TAILING OF SMEAR).
Error in neutrophil count.
WELL MADE
PERIPHERAL SMEAR
10.
11. Fix with in 4 hours.
Fix – acetone free methyl alcohol.
SMEAR FIXATION
12. Place smear on staining rack.
Pour undiluted stain on slide with dropper.
Stain has to cover entire upper surface.
Wait 2 min – methyl alcohol fixes the smear.
Pour double the amount buffer water.
Gently mix and wait 8 to 10 min.
Look for greenish metallic scum to appear.
Displace the stain – pouring running water from one side od slide – stain scum
drained off – no more stain left behind.
DO NOT DISCARD STAIN FROM SLIDE BY TILTING IT – RESULTS IN
STAIN PRECIPITATES.
Keep slide in slanting position.
Examine under microscope.
SMEAR STAINING
13. Buffered pH is important.
Acidic pH – cells pink – WBC light stained.
Basic pH – cells blue – WBC bluish black.
If overstained – wash with Leishman stain / methanol – few seconds.
If understained – restain – add Leishman stain and buffer.
SOURCE OF ERROR
14. Methanol fixed PS – pour diluted stain ( 1 in 10 ).
Wait 20 – 3- min.
Wash slide.
Dry it.
THICK FILM – MALARIA.
GIEMSA STAIN
15. RBC: size, shape, colour.
WBC: number and distribution – 100 cells count.
PLATELET: number and distribution.
HEMOPARASITES: malaria, microfilaria.
PS EXAMINATION