CURRICULUM VITAE-JP Alao

John Patrick Alao Curriculum Vitae
CURRICULUM VITAE
NAME: JOHN PATRICK ALAO BSc. (Hon), PhD.
ADDRESS: Marklandsgatan 65
41477 Gothenburg
Sweden
TEL: +46-76-008 09 65
D.O.B: 23/01/73 AGE: 43 yrs
NATIONALITY: GERMAN
SKILLS:
• Excellent team player who interacts well at all levels, influencing and inspiring
others, demonstrating initiative, innovation, critical thinking and determination to
solve problems and complete tasks. Attended graduate courses on laboratory
management and pedagogics.
• Comprehensive research and development experience in Cell Biology using cell
lines, yeast and murine xenograft models, Biochemistry, Molecular Cell Biology,
Assay and drug development and screening laboratories. Design and execution of
graduate lectures and practical classes.
• Excellent computing skills including, Microsoft Office (Word, Excel, PowerPoint),
Graphics (Adobe Photoshop) and use of analytical software (excel, prism, etc.)
• Supervision of research staff and graduate students on techniques and theoretical
aspects of their projects. Managing of allocated grant funding.
CURRENT POSITION:
Apr 2010 - Present Principal Research Scientist
Dept. of Chemistry and Molecular Biology
Lundberg Laboratory, Gothenburg University,
Box 462,
S-405 30 Gothenburg, Sweden.
Supervisor: Prof. Per Sunnerhagen
PREVIOUS APPOINTMENTS:
Jan 2009 - Apr 2010 Senior Postdoctoral fellow
Dept. of Cell and Molecular Biology
Box 462,
1

Jan 2007- Jan 2009 EMBO Postdoctoral fellow
Box 462,
Mar 2006 - Jan 2007 Postdoctoral Researcher
Dept. of Molecular Biology
Box 462,
Sept 2004 - Mar 2006 Postdoctoral Fellow
Department of Cancer Medicine, Imperial College London,
Hammersmith Hospital Campus, London, U.K.
Supervisors: Dr. D. M. Vigushin, Prof. R. C. Coombes
July 2002 - Sept 2004 Pre-doctoral Research Fellow,
As Above
July 1997 - Oct 1999 Research Assistant, Imperial College,
Department of Medical Oncology
Charing Cross Hospital, London, U. K.
Jan 1997 - May 1997 Laboratory Technician,
Redbridge Technical College, Science Department
Romford, Essex, U.K.
Apr 1996 - Aug 1996 Laboratory Technician,
GLAXO WELLCOME Operations, Dartford, Kent, U.K.
Feb 1995 - Sept 1995 Laboratory Technician,
As Above
Apr 1990 - Jun 1990 Laboratory Assistant,
International Institute of Tropical Agriculture (IITA),
Biochemistry Laboratory, Ibadan, Nigeria
2

EDUCATION:
Oct 1999 - Oct 2003 DOCTORAL DEGREE (PHD)
University of London, Imperial College of Science
Medicine and Technology, London, U.K., National
Institutes of Health, Bethesda, MD, USA.
Year of award: 2004
Area of study: Investigative medicine
Thesis title: Functional characterisation of RNF6: A
novel RING finger protein mapping
close to the t(4;13) breakpoint.
Supervisors: Dr. D. H. C. MacDonald
Dr. A. M. Weissman
Sept 1995 - July 1999 BSc. BIOCHEMISTRY (HON) DEGREE (2:1)
Units: Human Physiology, Pharmacology, Molecular
Biology, Cell Biology, Biotechnology,
Medical Biochemistry.
University of East London, London, U.K.
COURSES:
Oct 1999 Radiation safety course, Imperial College London, U.K.
Jul 2001 Radiation safety course, National Institutes of Health,
Bethesda, MD, U. S. A.
Aug 2002 Certificate in the use of small laboratory animals, Institute
of Biology, U.K.
Feb 2003 Postgraduate course: The cellular and molecular biology
of cancer.
Imperial College London, U.K.
Nov 2007 EMBO fellows laboratory management course, Leimen/
Heidelberg, Germany.
PEDAGOGIC TRAINING:
Jun 2013 Teaching and Learning in Higher Education 1: Basic
Course, 5 hec (HPE101)
Jun 2013 Teaching and Learning in Higher Education 2: Subject
Field Pedagogy, 5 hec (HPE102)
Jun 2013 Teaching and Learning in Higher Education 3: Applied
Analysis, 5 hec (HPE103)
Jun 2013 Supervision in Postgraduate Programs, 5 hec (HPE201)
3

PROFESSIONAL PROFILE
Scientific
Methods of molecular biology and biochemistry (including: cloning; PCR; RNA, DNA
and protein isolation; protein purification from E.coli; western blotting; mammalian
cells cultures and yeast, transfection, siRNA, immunofluorescence, FACS,
Bioscreens etc.)
Researched procedures needed to complete laboratory experiments
Prepared protocols for laboratory experiments
Investigated and interpreted observations and data
Manuscript preparation
Interpersonal
Good organisational, communications and presentation skills
Ability to work alone as well as in group settings
Ability to work under pressure
REFEREES: Prof. P. Sunnerhagen
Lundberg Laboratory
Göteborg University
Box 462
S-405 30 GÖTEBORG
SWEDEN
Tel.: +46 31 773 3830
Fax: +46 31 773 3801
E-mail: Per.Sunnerhagen@cmb.gu.se
Prof. S. Ali
Department of Oncology
Imperial College,
Hammersmith Hospital Campus,
London, W12 0NN
United Kingdom
Tel.: +44 20 7594 2811
E-mail: simak.ali@imperial.ac.uk
Dr. A. M. Weissman
Regulation of Protein Function Laboratory
NCI-Frederick,
P.O. Box B, Building 560,
Room 22-103,
Frederick,
MD 21702-1201,
U.S.A.
Tel.: +1 301-846-1222
Fax. +1 301-846-1666
E-mail: amw@nih.gov
PEER-REVIEWED ARTICLES:
4

1) J. P. Alao, A. M. Weber, A. Shabro, P. Sunnerhagen (2015) Suppression of
sensitivity to drugs and antibiotics by high external cation concentrations in fission
yeast. PLoS ONE 10:e0119297
2) J. P. Alao, S. Michlikova, P. Dinér, M. Grøtli, P. Sunnerhagen (2014) Selective
inhibition of RET mediated cell proliferation in vitro by the kinase inhibitor SPP86.
BMC Cancer 14: 853
3) I. Gumula , J. P. Alao, I. O. Ndiege, P. Sunnerhagen, A. Yenesew, M. Erdélyi
(2014) Flemingins G-O, Cytotoxic and Antioxidant Constituents of the Leaves of
Flemingia grahamiana. Journal of Natural Products 77: 2060- 2067
4) J. P. Alao, J. J. Sjölander, J. Baar, N. Özbaki-Yagan, B. Kakoschky,
P.Sunnerhagen (2014) Caffeine stabilizes Cdc25 independently of Rad3 in
Schizosaccharomyces pombe contributing to checkpoint override. Molecular
Microbiology 4: 777-96
5) N. Abdissa, M. Induli, P. Fitzpatrick, J. P. Alao, P. Sunnerhagen, G. Landberg, A.
Yenesew, M. Erdélyi (2014) Cytotoxic quinones from the roots of Aloe dawei.
Molecules 19: 3264- 3273
6) J-F. Poon, J. P. Alao, P. Sunnerhagen, P. Dinér (2013) Azastilbenes: A cut off
to p38 MAPK inhibitors. Organic & Biomolecular Chemistry 11: 4526- 4536
7) K. Jansson, J. P. Alao, K. Viktorsson, J. Warringer, R. Lewensohn, P.
Sunnerhagen (2013) A role for Myh1 in DNA repair after treatment with strand-
breaking and crosslinking chemotherapeutic agents. Environmental and Molecular
Mutagenesis 54: 327- 337
8) M. Endale , A. Ekberg , J. P. Alao, H. M. Akala, A. Ndakala, P. Sunnerhagen, M.
Erdélyi, A. Yenesew. (2012) Anthraquinones of the roots of Pentas micrantha.
Molecules 18: 311-321
9) M. Endale, A. Ekberg, H. M. Akala, J. P. Alao, P. Sunnerhagen, A. Yenesew, M.
Erdélyi. (2012) Busseihydroquinones A-D from the Roots of Pentas bussei. Journal
of Natural Products 75: 1299- 1304
10) P. Dinér , J. P. Alao, J. Söderlund , P. Sunnerhagen , M. Grotli (2012)
Preparation of 3-Substituted-1-Isopropyl-1H-Pyrazolo[3,4-d]Pyrimidin-4-Amines as
RET Kinase Inhibitors. Journal of Medicinal Chemistry 55: 4872- 4876
11) M. Endale, J. P. Alao, H. M. Akala, N. K. Rono, F. L. Eyase, S. Derese, A.
Ndakala, M. Mbugua, D. S. Walsh, P. Sunnerhagen, M. Erdelyi, A. Yenesew
(2012) Antiplasmodial Quinones from Pentas longiflora and Pentas lanceolata
Planta Medica 78: 31-35
5

12) C. Dyrager, L. N. Möllers, L. K. Kjäll, J. P. Alao, P. Dinér, F. Wallner, P.
Sunnerhagen, M. Grotli (2011) Design, Synthesis and Biological Evaluation of
Chromone-based p38 MAP Kinase Inhibitors. Journal of Medicinal Chemistry
54: 7427- 7431
13) J. P. Alao, P. J. Huis in’t Veld, F. Buhse, P. Sunnerhagen (2010) Hyperosmosis
enhances radiation and hydroxyurea resistance of S. pombe checkpoint mutants
through the spindle checkpoint and delayed cytokinesis. Molecular Microbiology
77: 143- 157
14) J. P. Alao, P. Sunnerhagen (2009) The ATM and ATR inhibitors CGK733 and
caffeine suppress cyclin D1 levels and inhibit cell proliferation. Radiation Oncology
4: 51
15) J. P. Alao, J. Olesch, P. Sunnerhagen (2009) Inhibition of type I histone
deacetylase increases resistance of checkpoint deficient cells to genotoxic agents
through mitotic delay. Molecular Cancer Therapeutics 8: 2606- 2615
16) J. P. Alao (2009) The ATM regulated DNA damage response pathway as a
chemo- and radiosensitisation target. Expert Opinion on Drug Discovery 4: 495-
505
17) J. P. Alao, P. Sunnerhagen (2008) Rad3 and Sty1 function in S. pombe: an
integrated response to DNA damage and environmental stress? Molecular
Microbiology 68: 246- 254
18) J. P. Alao (2007) The regulation of cyclin D1 degradation: Roles in cancer
development and the potential for therapeutic invention. Molecular Cancer 6: 24
19) C.M. Marson, T. Mahadevan, J. Dines, S. Sengmany, J.M. Morrell, J. P. Alao, S.
P. Joel, D. M. Vigushin, R. C. Coombes (2007) Structure-activity relationships of
aryloxyalkanoic acid hydroxyamides as potent inhibitors of histone deacetylase.
Bioorganic and Medicinal Chemistry letters 17: 136- 141
20) J. P. Alao, A. V. Stavropoulou, E. W.-F. Lam and R. C. Coombes (2006) Role of
glycogen synthase kinase 3 beta (GSK3b) in mediating the cytotoxic effects of the
histone deacetylase inhibitor trichostatin A (TSA) in MCF-7 breast cancer cells.
Molecular Cancer 5: 40
21) J. Leyton, J. P. Alao, M. Da Costa, A. V. Stavropoulou, J. R. Latigo, M.
Perumal, R. Pillai, Q. He, P. Atadja, E. W.-F. Lam, P. Workman, D. M. Vigushin, E.
O. Aboagye (2006) In vivo biological activity of the histone deacetylase inhibitor
LAQ824 is detectable with 3´- deoxy- 3’- [16
F] fluorothymidine positron emission
tomography. Cancer Research 66: 7621- 7629
22) J. P. Alao, A. V. Stavropoulou, E. W.-F. Lam, R. C. Coombes, D. M. Vigushin
(2006) Histone deacetylase inhibitor, Trichostatin A induces ubiquitin-dependent
cyclin D1 degradation in MCF-7 breast cancer cells. Molecular Cancer 5: 8
6

23) J. P. Alao, S. C. Gamble, A. V. Stavropoulou, K. M. Pomeranz, E. W.-F. Lam,
R. C. Coombes, D. M. Vigushin (2006) The cyclin D1 proto-oncogene is
sequestered in the cytoplasm of mammalian cancer cell lines. Molecular Cancer 5:
7
24) M. Lucey, D. Chen, J. Lopez-Garcia, S. Hart, F. Phoenix, R. Al-Jehani, J. P.
Alao, R. White, K. Krindle, R. Losson, P. Chambon, M. Parker, P. Schar, D. Heery,
L. Buluwela, S. Ali (2005) T:G Mismatch-specific Thymidine-DNA Glycosylase as a
coregulator of transcription interacts with SRC1 family members through a novel
tyrosine repeat motif. Nucleic Acids Research 33:6393-6404
25) R. Varshochi, F. Halim, A. Sunters, J. P. Alao, P. A. Madureira, S. M. Hart, S.
Ali, D. M. Vigushin, R. C. Coombes, E. W. Lam (2005) ICI182,780 induces p21waf1
gene transcription through releasing HDAC1 and ERα from Sp1 sites to induce cell
cycle arrest in MCF-7 breast cancer cell line. Journal of Biological Chemistry
280: 3185-3196
26) J. P. Alao, E. W. Lam, S. Ali, L. Buluwela, W. Bordogna, P. Lockey, R.
Varshochi, A. V. Stavropoulou, R. C. Coombes, D. M. Vigushin (2004) Trichostatin A
inhibits breast cancer cell proliferation through estrogen receptor dependent
transcriptional repression and estrogen receptor independent proteasomal
degradation of cyclin D1. Clinical Cancer Research 10: 8094-8104
27) L. Sanderson, G. W. Taylor, E. O. Aboagye, J. P. Alao, J. R. Latigo, R. C.
Coombes, D. M Vigushin (2004) Plasma pharmacokinetics and metabolism of
the histone deacetylase inhibitor trichostatin A after intraperitoneal
administration to mice. Drug Metabolism and Disposition 32: 1132-1138
28) D. M Vigushin, Y. Dong, L. Inman, N. Peyvandi, J. P. Alao, C. Sun, S. Ali, E. J.
Niesor, C. L. Bentzen, R. C. Coombes (2004) The Nuclear Oxysterol Receptor
LXRα is expressed in the normal human breast and in breast cancer. Medical
Oncology 21: 123-131
29) C. M. Marson, N. Serradji, A. S. Rioja, S. P. Gastaud, J. P. Alao, R. C.
Coombes, D. M. Vigushin (2004) Stereodefined and Polyunsaturated
Inhibitors of Histone Deacetylase based on (2E,4E)-5-Arylpenta-2,4-dienoic
Acid Hydroxyamides. Bioorganic and Medicinal Chemistry letters 14: 2477-
2481
30) J. P. Alao, M. Q. Mohammed, S. Retsas (2002) The CDKN2A tumour
suppressor gene: no mutations detected in patients with melanoma and additional
unrelated cancers. Melanoma Research 12: 559-563
31) J. P. Alao M. Q. Mohammed M. J. Slade, S. Retsas (1999) Detection of
tyrosinase mRNA by RT-PCR in the peripheral blood of patients with advanced
metastatic melanoma. Melanoma Research 9: 395-399
PEER-REVIEWED CONFERENCE CONTRIBUTIONS:
1) J. P. Alao, P. Sunnerhagen (2014) The impact of caffeine mediated Cdc25
stabilization on cell cycle kinetics in Schizosaccharomyces pombe. FEBS-EMBO
2014
7

2) J. P. Alao, A. M. Weber, P. Sunnerhagen (2013) Modulation of fission yeast
drug sensitivity by environmental and intracellular potassium ion concentrations. 7th
International Fission Yeast Meeting
3) J. P. Alao, N. Özbaki, J. J. Sjölander, B. Kakoschky, P. Sunnerhagen (2011)
Caffeine overrides fission yeast DNA damage and replication checkpoints by
inducing Cdc25 accumulation. 6th
International Fission Yeast Meeting
4) J. Alao, P. Sunnerhagen (2009) Rad3 regulates the cell cycle stress response in
Schizosaccharomyces pombe. 5th
International Fission Yeast Meeting (Oral
presentation).
5) J. Alao, P. Sunnerhagen (2009) Rad3 regulates the cell cycle stress response in
Schizosaccharomyces pombe. 3rd
Congress of European Microbiologists
6) J. P. Alao, A. V. Stavropoulou, E. F.-W. Lam, R. C. Coombes, D. M. Vigushin
(2005) Ubiquitin dependent degradation of cyclin D1 occurs via distinct nuclear and
cytoplasmic pathways in MCF-7 breast cancer cells treated with the histone
deacetylase inhibitor Trichostatin A. Proceedings of the American Association
for Cancer Research (Abstract No. 2274).
7) J. Leyton, J. P. Alao, J. R. Latigo, M. Perumal, Q. He, D. M. Vigushin, P. Atadja,
E. O. Aboagye (2005) Early detection of tumor response to LAQ824 by 3’-Deoxy-3’-
[18
F]fluorothymidine by positron emission tomography in HCT116 tumor bearing
mice. Proceedings of the American Association for Cancer Research (Abstract
No. 3814).
8) J. P. Alao, E. W. Lam, S. Ali, W. Bordogna, P. Lockey, R. C. Coombes, D. M.
Vigushin (2004) Estrogen receptor dependent and independent mechanisms of
cyclin D1 downregulation by the histone deacetylase inhibitor trichostatin A.
Proceedings of the American Association for Cancer Research (Abstract No.
2444/ oral presentation).
9) J. P. Alao, M. Q. Mohammed, S. Retsas (2002) The CDKN2A tumour suppressor
gene: no mutations detected in patients with melanoma and additional unrelated
cancers. Melanoma Research 12: A1
10) J. P. Alao, M. Q. Mohammed, M. J. Slade, S. Retsas (1999) Detection of
tyrosinase mRNA by RT-PCR in peripheral blood of patients with melanoma.
Melanoma Research 9: 334
BOOK CHAPTERS:
1) J. P. Alao (2008) Review: Cyclin D1 as a Therapeutic Target in Cancer.
Oncogene proteins: New Research. p. 99-128. ISBN 978-1-60456-290-3 New
York: Nova Science Publishers, Inc.
2) J. P. Alao (2009) G1 Phase cyclins in cancer development and progression.
Checkpoint controls and targets in cancer therapy, Cancer drug discovery and
development. p. 123-153. ISBN-13: 978-1-60-761177-6 New York: Humana Press.
3) J. P. Alao (2012) Caffeine, cyclin D1 and cell proliferation. Tea in health and
disease prevention. p. 1159- 1171. ISBN 978-0-12-384937-3 London: Elsevier.
8

SUBMITTED SEQUENCES:
1) Alao, J. P., Grand, F. H., MacDonald, D. H. C. (2000) GenBank Direct
Submission: Accession no. AF293342 Homo sapiens RNF6 protein (RNF6)
mRNA, complete cds, alternatively spliced.
2) Stavropoulou, A.V., Alao, J. P., Lam, E. W. F., Coombes, R. C. and Vigushin, D.
M. (2005) GenBank Direct Submission: Accession no. DQ223959
Homo sapiens F-box and leucine-rich repeat protein 20 variant b mRNA, complete
cds, alternatively spliced.
MISCELLANEOUS:
Reviewer: International Journal of Cancer, Biochemical Pharmacology, Oncogene,
Molecular Cancer, Expert Opinion on Drug Discovery, International Journal of
Dermatology, Mutation Research/Fundamental and Molecular Mechanisms of
Mutagenesis, Cell Cycle, BMC Cancer, PLoS One, Oncotarget
PROJECT GRANTS AND AWARDS:
EMBO Long-term postdoctoral fellowship 2006
Werner Gren postdoctoral fellowship 2006
American Association for the Advancement of Science (AAAS) / Science Program
for Excellence in Science Award 2007
Assar Gabrielsson’s fund for clinical cancer research 2007
Carl Trygger’s fund for scientific research 2010
Percy Falks fund for prostate and breast cancer research 2011
Knut and Alice Wallenbergs fund 2012
Percy Falks fund for prostate and breast cancer research 2013
Wilhelm and Martina Lundgrens fund 2013
FEBS EMBO Travel bursary 2014
Lars Hiertas Minne fund 2014
Carl Trygger’s fund for scientific research 2015
9

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