1. Koh Tze Loong
PERSONAL PARTICULARS
Full Name : Koh Tze Loong
Passport/ NRIC No. : S8124394H
Gender : Male
Nationality : Singaporean
Race : Chinese
Marital Status : Single
NS Status : Completed
Religion : Buddhist
Language Proficiency : English, Chinese
CONTACT DETAILS
Email : kohtzeloong@gmail.com
Main Contact : 96368336 (HP), 65423838 (home)
Address : 38 Sunbird Circle Apollo Garden
Singapore 487277
EDUCATION and EMPLOYMENT - SUMMARY
Highest Degree : Masters of Science
Course of Study : Masters
Graduation : May 2014
Years Worked : 7 Years
Work Experience : 2 Years employed in A-star IMCB and ETC as research assistant
5 Years employed in NUH under A/Prof Chng Wee Joo as research
assistant. Completed Masters program under NUS in May 2014.
1.5 Years employed in GSK Tuas as QA executive for Sterility
Assurance.
Employment Status : Research Assistant Hematology-Oncology Department
Working for Assoc Prof Chng Wee Joo
38 Sunbird Circle
Singapore 487277
96368336
kohtzeloong@gmail.com

2. EDUCATION - DETAILS
Masters of Science (National University Singapore) June 2011 - May 2014
University of Queensland, Australia
Honours (1st Class), Bachelor of Biotechnology Feb 2004 – Jul 2006
Dean’s List, Semester 3, 2005
High Distinction for Molecular Microbiology
Distinctions for: Genomics & Bioinformatics,
Temasek Polytechnic
Diploma in General Biotechnology Jul 1998 – Jul 2001
Manjusri Secondary School
GCE ‘O’ Level Feb 1994 – Dec 1997
EMPLOYMENT - DETAILS
Honors Project with Associate Professor John Maxwell Pemberton July 2005 – June 2006
Fulfillment as part of the Honors Program. Did genetic work on
bacteria: Pseudomonas stutzeri, Rhodobacter sphaeroides and Escherichia Coli.
A-Star Bioinformatics Institue (BII) October 2006 – August 2007
Worked for 1.5 Years under Dr Jeffery Hill and Dr Francis Lim Buan Hong
in A-STAR Bioinformatics Institute. Did collaborations work there with
IMCB. Worked on the cloning and expression of artificial proteins.
ETC (Experimental Therapeutics Center) August 2007 – October 2008
Transferred to ETC with Dr Jeffery Hill, there I helped to set up new
laboratory. Did cloning and expression of AIF-SH2 (Apoptosis Inducing
Factor-Short version) and AIFM3 (mitochondrion associated) into expression
vector Pet21b and expressed in E. coli cell RGB-PlysS (Rosetta-gami B)
NUH November 2008 – August 2014
Worked under Assoc. Professor Chng Wee Joo.
My duties include
- Immunohistochemistry for multiple collaborators,
- Microarray experiments for our own lab publications and
- Various forms of drug studies involving anti-cancer drugs like
DZNep, Velcade and other test drugs from pharmaceutical companies.

3. GSK August 2014 - Current
Currently working in GSK Vaccines as a Quality Assurance Executive in the Sterility Assurance group.
Involved in:
- Tracking Environmental Monitoring Trends for presentation in quarterly reviews.
- Handling various deviations in production with relations to environmental out of specifications
or aseptic related deviations.
- Oversight of Media Simulations in the isolator for operator and equipment re-qualification
- GAP analysis of various sterility related guidelines such as 6003-1 to 6003-4 series. (sterility,
environmental monitoring and aseptic simulations)
- Training and qualification of aseptic operators in the site
- Writing of rationale papers for Aseptic Media Simulations
- Various projects on site with relation to single use systems (Lynx Connectors, Single use bags)
Supervisory experience:
Currently designated as backup for team QA Lead. When Lead is absent, duties involve:
- Conducting Tier 1 meetings for SA technicians as well as representing the team in discussions
with other teams from production and quality control.
- Organizing team schedule and leading technicians in walk-downs of production areas for
sampling as well as during QA oversight.
- Tracking of Environmental monitoring frequency conducted by QA technicians as well as
ensuring all sampling is conducted on track.
- Handling any deviations pertaining to usage of expired agar, invalid sampling etc.
Oversight of Media simulations involves making critical decisions with regards to invalidating samples
or during spillages or leakage etc.
Currently the SA representative for 1 of 2 buildings in the site, able to make independent decisions with
regards to any matters involving Environmental or Sterility matters such as but not limited to:
- Shutdown cleaning frequency.
- Actions in the event of HVAC stoppage or pressure cascade.
- Out of action levels for biobuden found in CIP samples for various equipments such as SEC
columns, fermenters, fundabacs.

4. OTHER RELEVANT SKILLS
Bioinformatics : DNA-Star, Blast Program, TIGR, NCBI, Cluster W,
Primers Designing, Gene Spring Analysis platform.
Laboratory Techniques : RT-PCR, Real-Time PCR, Protein Expression and purification
(AIF-SH2, MMP-QCAT) PCR, DNA Sequencing, Plasmid
Extractions, Bacterial Cloning, Cell staining with antibodies,
Human cell tissue Culture (Myeloma cell lines, Leukemia
cell lines, Breast Cancer, Colon Cancer), Western Blot,
Microarray Platforms : Agilent miRNA chips, Illumina Whole Genome
DASL chips, Affimetrix 3’IVT labeling chips
ImmunoHistoChemistry:Staining with H&E (Hematoxylin and eosin), Antibody staining with
various antibodies (CD31, VWF factor, LIN-28 and PRL-3).
Creation of Tissue Microarrays from cell blocks and various tissues.
Flow Cytometry :Worked with BD LSR II machine and performed Apoptosis
Assays using FITC Annexin V Apoptosis Kit.
Sterility Assurance :Familiar with Isolator operations as well as various SOPs related to isolator
use.
Familiar with SAP user system.
Familiar with various sterility guidelines as well as media simulation
requirements.
Familiar with GSK shutdown, line clearance, performance qualifications,
validation procedures and change request process.
OTHER SKILLS
Computer Applications : Microsoft Office Applications
Driving : Class ‘3’ License
National Service : Completed June 2001 – Dec 2003
Current salary : SGD $4,900
Expected Salary : Negotiable