Stem cell technology

1. Stem Cell Technology in
Reproduction
Dr. Manna Baruti
L-2017-V-10-D

2. Introduction to Stem Cell
➢Undifferentiated cells.
➢Retain the capacity for self-renewal.
➢Can differentiate into specialized cell types

5. Totipotent Pluripotent Multipotent Progenitor
cells
•Embryonic
blastomeres.
•From fertilization
up to 8 cells stage
•Give complete
organism including
extra embryonic
tissue.
•From embryonic
tissue layers;
(ectoderm,
endoderm and
mesoderm).
•Two types:
Embryonic stem
(ES)
Embryonic Germ
(EG)
•Differentiate into
more than 1 type of
specialized cells.
Hematopoietic
stem cells
•Produce terminally
differentiated or
specialized cells.

6. Types of Stem Cell
❖ Embryonic Stem cell (ESC)
❖ Adult Stem Cell (ASC)

9. Cord blood Stem Cell and Amniotic Fluid Stem cell
● 2005 - Cord blood Embryonic Stem cell- Kingston university
● 2007 - Dr. Anthony Atala claimed that a new type of stem cell
had been isolated in amniotic fluid.

10. Induced Pluripotent Stem cell

12. Yamanaka's group successfully transformed human
fibroblasts into iPSCs with the same four pivotal genes,
OCT4, SOX2, KLF4, and C-MYC, using a retroviral system.
Thomson and colleagues used a different set of factors,
OCT4, SOX2, NANOG, and LIN28, using a lentiviral system.

13. Stem Cell and Neo-Oogenesis
Jhonson et al. 2004-Nature
Germline Stem cell from Ovarian surface epithelium.
GSC is inducing Post natal neo folliculogenosis.
In 2005 from bone marrow and cord blood cell.
PGCs/oogonia or PGC-derived undifferentiated cells with GSC characteristics
remain in the postnatal and adult ovary and under certain conditions may
resume mitosis, enter meiosis and give rise to oocytes.

14. Ovarian Stem cell Epithelium
Bone Marrow
Cord blood cell
Doxorubicin Treatment to Ovary

15. Liu 2007 china
Gene Expression study in different level of meiosis in human
ovary.
Ji Wu - 2009 - Shanghai
Offspring from Neonatal Ovarian Germ line Mouse Vasa
Homologue.

16. Stem Cell in Animal Production
2002 Vet Stem Inc
Veterinary Regenerative Medicine
3000 Horses
1000 Dogs

18. Major Types of stem cell in Animals
Embryonic Germ Cell
Amniotic Fluid Stem Cell
Amniotic Membrane Cells
Fetal Fibroblast
Umbilical cord
Wharton’s jelley stem cell
Placenta Stem cell

19. Stem Cell in Male Fertility
Spermatogonial Stem Cell
Enzymatic digestion for recovering stem cell
Sertoli cell feeder layer
0.03% isolated from Mammalian testis
Xenotransplantation

20. Deep study of Testicular Histochemistry.
Azoospermia.
Iatrogenic Effect of Clinical treatment of Cancer.

22. Stem Cell in Female Infertility
2010 First Artificial human Ovary.
Organised Granulosa and Thecal cell.
Endomatrial Transplantation

23. Tissue Engineered in Gynaecology
Uterus transplantation-2002
Bone marrow stem cell IU in endomatrium.

24. Prenatal Stem cell Transplantation for IU treatment
of congenital diseases of fetus
● Autologous fetal stem cell
● Prenatal implantation
● Ultrasound Guided

25. Stem cell in Reproductive tract
Stem Cell and Cattle Breeding-Georgia
Caire Technique
Sustained fertility
Green thinks stem cell transfers

26. Stem Cells and the endometrium
Asherman’s Syndrome
Bone Marrow Derived Stem cell infusion
Jing et al. 2014, Kilic et al. 2014

27. Stem Cell and Vaginal reconstruction
Vaginal Prolapse
Vaginal Fistula
Rejection of Graft
Muscle Derived Stem Cell(Oshima et al. 2005)
Successful in Vaginal Tissue regeneration.

28. Stem cell milestone in Veterinary Reproduction
1. Iwaski et al 1990 ICM proportion lower in bovine IVF embryos than in vivo produced
2. Saito et al 1992 Bovine embryonic stem cells grow slower than murine embryonic
stem cells
3. Hamers et al 1995 Bovine culturing embryo derived cells resemble epithelial cells
rather than pluripotent ICM cells
4. Talbot et al 2000 Bovine embryonic cells grew as cell sheets. Passaging by physical
dissociation

29. 1. Li et al 2003 Porcine ICMs isolated by enzymatic method. Two embryonic cell lines
cultured upto 6 passages
2. van Eijk et al 1999 Bovine Oct 4 pseudogene isolated
3. Wang et al 2005 ES cells from cloned bovine embryos with marker staining pattern
similar to bovine blastocysts
4. Yadav et al 2005 Bovine ICM derived cells express the Oct4 ortholog

30. ● Animal model testing for pharmaceutical research
● Use of stem cells in transplantation and cell replacement therapy
● Conservation of endangered species
● Understanding fundamental events in embryonic development
● Therapeutic delivery system
● To resolve mysteries of developmental biology
● To investigate genes involved in differentiation and development
● Cell banking for research applications

31. Conclusion
Revolution to Medicine
Production of Stem cell line
Embryonic stem cell isolation
Preservation of Germ line of supirior bull and transper
stem cell to recipient.

32. References
1. Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined
Factors.Kazutoshi Takahashi, Koji Tanabe, Mari Ohnuki, Megumi Narita, Tomoko
Ichisaka, Kiichiro Tomoda, and Shinya Yamanaka.
2. Ovarian stem cells and neo-oogenesis: A breakthrough in reproductive biology
research. Veterinary World, 2011, Vol.4(2):89-91.
3. Dedifferentiation: A New Approach in Stem Cell Research. SA CAI, XIAOBING
FU, AND ZHIYONG SHENG.
4. Human induced pluripotent stem cells and male infertility: an overview of current
progress and perspectives.Fang Fang Zili Li Qian Zhao Honggang Li Chengliang
Xiong.

33. 5. CELL TECHNOLOGIES IN REPRODUCTOLOGY, OBSTETRICS AND
GYNECOLOGY. Nasadyuk C. M. Institute of Cell Therapy, Kyiv, Ukraine.
6. In vitro culture and characterization of spermatogonial stem cells on Sertoli cell
feeder layer in goat (Capra hircus). R. Kumar Pramod & Abhijit Mitra.
7. Stem cells in reproductive medicine:ready for the patient?. Human
Reproduction, Vol.0, No.0 pp. 1–8, 2015.
8.Johnson, J., Canning, J., Kaneko, T., Pru, J.K and Tilly, J.L. (2004). Germline
stem cells and follicular renewal in the the postnatal mammalian ovary. Nature
428,145–150.

34. Thank You