Gene Transfer, Types of it, Experiment related to it, Conjugation and its types and how they become F -ve to F +ve and F+ve to Hfr, Transduction and its Types

1. KUNAL
17373016
1st YEAR IInd SEMESTER
MOLECULAR GENETICS

2. Eg. PROKARYOTES Eg. REPRODUCTION
TRANSDUCTIONCONJUGATION TRANSFORMATION

3. Exchange of genetic material b/w bacteria
 Drug resistance.
Genetic material exchanged via Viruses.
 Drug resistance
Uptake of naked DNA molecule or fragment
from the medium by a cell and incorporation
into recipient.
 Antibiotic resistance
 Pesticide resistance in crops

5. Bacterial conjugation is the transfer of genetic material between bacteria through
direct cell-to-cell contact, or through a bridge-like connection between the two cells.
It occurs in both gram positive and gram negative cells but the mechanism is quite
different.

6. C
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A
T
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8. The transfer of genetic material from one cell to another cell by a bacteriophage
is called Transduction .
• Transduction has been found to occur in variety of prokaryotes , including
certain species of Bacteria : Escherichia , Pseudomonas, Salmonella ,
Staphylococcus , etc.
• The bacteriophage containing the bacterial DNA is called transduced DNA .

9. Generalized Transduction-
Transduction in which potentially
any donor bacterial gene can be
transferred.
 Occurs during lytic cycle.
 Replicate Immediately.
 No viral DNA.
Specialized Transduction -
Transduction in which only certain
donor genes can be transferred.
 Occurs during lysogenic cycle.
 Replicate later.
 Viral and bacterial DNA.