This research is carried out in order to improve the production of eggs in indigenous chicken by reducing the inter-sequence stopped days through use of anti-prolactin agent (Bromocriptine) and serum from laying hen. Sixty-four indigenous (deshi) chickens of 20-22 weeks of age, were randomly assigned into four groups (i, j, k and l) and each group consisting of 16 hens. Control was designated as Group I and Bromocriptine orally at a dose of 641μg/bird/day was used to treat group j, group k was treated with serum of laying kadhaknath hen serum at a dose of 1 ml intramuscularly/bird/day and group l was treated with both Kadhaknath serum and Bromocriptine at doses given to group j and k for the period of 15 March, 2019 to 16 June, 2019 and egg production, stopped days, prolactin level, hematological parameter and egg qualities were observed. A significant increase (p<0.05) in Egg production was noticed in all treated groups in comparison to the groups which were in non- treated control and group k showed the highest production. All treatment groups depicted a significant decrease (p<0.05) in stopped days and prolactin levels and lowest were observed in hens of group l. In hematological values between the chicken group, no significant differences were noticed. The present study reveals that combined treatment with Bromocriptine and serum from laying kadhaknath hen increases egg production without affecting the health of indigenous chickens.

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[831]
EFFECTS OF LAYING KADHAKNATH HEN SERUM AND ANTI-PROLACTIN
MEDICATION [BROMOCRIPTINE] ON EGG YIELD OF INDIGENOUS
CHICKEN IN INDIA
Obul Reddy Praneeth Kumar Reddy*1
*1Masters In Biochemistry, Department Of Biochemistry YVU, India.
ABSTRACT
This research is carried out in order to improve the production of eggs in indigenous chicken by reducing the
inter-sequence stopped days through use of anti-prolactin agent (Bromocriptine) and serum from laying hen.
Sixty-four indigenous (deshi) chickens of 20-22 weeks of age, were randomly assigned into four groups (i, j, k
and l) and each group consisting of 16 hens. Control was designated as Group I and Bromocriptine orally at a
dose of 641μg/bird/day was used to treat group j, group k was treated with serum of laying kadhaknath hen
serum at a dose of 1 ml intramuscularly/bird/day and group l was treated with both Kadhaknath serum and
Bromocriptine at doses given to group j and k for the period of 15 March, 2019 to 16 June, 2019 and egg
production, stopped days, prolactin level, hematological parameter and egg qualities were observed. A
significant increase (p<0.05) in Egg production was noticed in all treated groups in comparison to the groups
which were in non- treated control and group k showed the highest production. All treatment groups depicted a
significant decrease (p<0.05) in stopped days and prolactin levels and lowest were observed in hens of group l.
In hematological values between the chicken group, no significant differences were noticed. The present study
reveals that combined treatment with Bromocriptine and serum from laying kadhaknath hen increases egg
production without affecting the health of indigenous chickens.
Keywords: Bromocriptine; Egg Production; Hematological; Indigenous Chicken, Laying Kadhaknath Hen
Serum.
I. INTRODUCTION
India has a wide range of chicken, primarily indigenous, described. About 80% of the total population of 1.366
billion is living in the 664,369 villages of India and almost each and every village home holds 6 to 10 chickens.
Its production cost is also very low due to low nutritional demand, high disease resistance to indian diseases.
Desi chickens from India have been found to be low producers with mature body weights in the range: 1.3 to
1.4kg (Howlider and Barua, 1990).Egg production ranged from 45-62/chicken/year (Alam et al., 2014).
Nevertheless, Khan (1983) claimed that Desi hen egg production may be improved to 150 eggs annually by bird
with a correct selection program, and are more resistant to Indian diseases and severe climates. If indigenous
poultry farming can be developed properly, India will be benefited. The very small size and long stopping are
major hindrances in this indigenous farming system.
It is the combination of the environment, genetics, and endocrine system that causes the development of
broodiness to happen (Sharp, 1989). A rise in the level of prolactin in the plasma is linked to the development
of broodiness (Sharp et.al., 1988) as egg laying stops, a fall in plasma LH and ovarian steroids were observed.
The production of prolactin has been reduced by active and/or passive immunization, particularly in Aseel and
bursa chickens, in order to avoid a reduction in prolactin during and before the preovulatory LH surge
development of broodiness (Scanes et.al., 1977; Scanes and colleagues, 1977).
The biological impact of prolactin inhibition in mammals is studied with the use of a dopamine agonist -
bromocriptine - to evaluate whether it has any effect (Horth and Farmer, 2000).Furthermore, serum collected
from the laying kadhaknath hen also might increase the FSH and LH Level of that hen simultaneously; in that
way, it would be possible to increase the egg yield by enhancing the laying period reducing the brooding
tendency to that hen.
Limited research had been performed in India in this aspect; therefore, the following objectives influenced the
conduct of the current research:
• Toobserve the number of egg production, egg quality and stopped days
• Toevaluate the hematological changes and hormonal levels of the chickens

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[832]
• Toknow the adverse effects related to treatment.
II. MATERIALS AND METHODS
To test the efficacy of anti-prolactin drug (bromocriptine) and serum from egg laying hen on native chickens,
sixty indigenous (deshi) chickens of 20-22 weeks age were used in this study and these were randomly
assigned into four groups (i, j, k and l) and each group consisting of 16 hens. The control was group i, and Group
j was treated using Bromocriptine orally at a dose of 641μg/bird/day, group k was treated with serum of laying
hen at a dose of 1 ml intramuscularly/bird/day and group l was treated with both serum and Bromocriptine at
doses given to group k and group l for the period of 15 March, 2019 to 16 June, 2019. The chickens are housed
under normal husbandry condition. All the birds were fed on traditional diets- broken rice, wheat 65gm per day
per bird as well as the fresh water ad libitum. Egg production, stopped days, prolactin level, hematological
parameter and egg qualities were observed for 12 weeks.
Serum samples from laying hens (indigenous) were isolated according to standard procedure. Briefly, the blood
samples were taken from the vein in the wing of a laying hen with a 3ml needle and then transferred into
Eppendorf tubes and allowed to coagulate for a period of 15 to 20 minutes. Then at 3500 RPM the blood is
centrifuged for nearly 15-minutes, then the serum was stored by separating from the sample on 4oC. 1ml of
serum was used intramuscularly per day per bird. There, ten birds remain for serum donor and no adverse
effects have not found at the site of injection.
For every hen, the egg production activity was noted on the same time, every day during their laying period.
The possibility of soft-shell or broken eggs were recorded and well identified. All the characteristic of the Egg
qualities such as egg albumin diameter, egg width, yolk width, yolk height, thick albumin height, shell thickness
of the egg, weight of fresh yolk, weight of fresh albumin, weight of dry shell, and the egg weight, were measured.
To determine the quality of the egg, the weight is recorded using an electronic weighing balance. Width and
length of the egg is estimated using the slide caliper. Eggs were broken down carefully on a 40×21m glass plate
for determining the qualities of the internal egg. Blood samples were collected and “Erythrocyte Sedimentation
Rate” (ESR), “Packed Cell Volume” (PCV), “Hemoglobin estimation” (Hb), “Total Erythrocyte Count” (TEC), were
measured according to standard procedures. Plasma prolactin level was measured from plasma isolated from
blood at the end of experiment by radioimmunoassay. ANOVA analysis was carried out on the data following a
Randomized Design with factorial arrangement of treatment and time (Torrie and Steel, 1986). SPSS program
was used for the analysis.
III. RESULTS AND DISCUSSION
• Egg production and stopped days
Egg productions and stopped days of different groups of hens are presented in Table 1.The study revealed that
the combined treatment significantly (p≤.0.05) affects the egg production. The Egg production also increased
either in single bromocriptine treated or serum treated group (Table 1), indicating that the individual
treatment was effective enough to promote the egg production. The results in this study agrees with those
outcomes of Reddy et.al. (2001) and David et.al. (2003).
Stopped days were lowest in group of hens treated with both Bromocriptine and serum from egg laying hen.
The combined treatment significantly (p≤.0.05) affect the stopped days. Pa days also decreased due to single
Bromocriptine or serum therapy, indicating that the treatment was effective enough to reduced the stopped
days. The present results are agreed with other results (Reddy et al., 2006).
• Prolactin level
Prolactin levels of different groups of hens are presented in Table 2. All treated groups showed a lowered
prolactin concentration than non treated control group. There was a decrease in the plasma prolactin levels
from 479.3±14.89 ng/ml to 147.9±5.06ng/ml during experimental period. The hen treated with antiprolactin
drug plus serum (Group D) have reduced prolactin level among the other groups and it is significant at 5%
(p<0.05) level. Same results were also noticed by Reddy et al. (2006), Chen et al. (1997), El-Halawani et.al.
(1995) & Youngren et al. (1991).

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• External and internal egg quality
On average values, such as the egg width (mm), egg length (mm), thick albumin height (mm), yolk width (mm),
shell thickness (mm), fresh yolk wt (gm), shell dry weight (gm) of different groups of hens showed no
significant difference (P<0.05) either treated with Bromocriptine or serum treatment (Table 3). As per the
outcomes it can be seen that treatment using Bromocriptine or serum from egg laying kadhaknath hen has no
negative impacts in the internal and external egg quality.
• Hematological parameter
Table 4 below lists the results of different parameters of the blood. Value of TEC, Hb content, PCV and ESR from
both the control and treated group same, with all the values under a normal range. Although the values showed
only a minor change in different groups however are not significant statistically (p>0.05).
• Postmortem examination
As per the study, no significant change in pathology was observed in any of the organs of any deshi chicken
among the treated groups. In addition, increased the number of follicles were observed in ovaries of those
treated groups.
Table 1. Effect of laying kadhaknath hen serum and bromocriptine on egg production
and stopped days
Group Egg/Clutch Stopped day
Total egg
production
Total stopped day
i 10.00a±0.72 35.00a ± 1.79 18.00c ±1.09 6.200a+_2.49
j 12.00a±1.84 9.00bc ± 0.42 47.00a ± 2.49 3.600bc+_2.167
k 10.00a±0.72 12.00b ±1.09 38.00b ± 1.88 42.00b+_2.46
l 11.00a± 0.93 8.000c ± 0.42 48.00a ± 1.42 32.00c+_1.478
Different superscripts values in same column are significant statistically (P<0.05).
Table 2. Effect of laying kadhaknath hen serum and bromocriptine on prolactin
Group Mean ± Standard Error of mean
Hormonal level of prolactin ng/ml
i 479.3a ± 14.90
j 178.8c ± 3.01
k 245.3b ± 3.83
l 147.9d ±5.06
Different superscripts values in same column are significant statistically (P<0.05).
Table 3. Effect of bromocriptine and laying Kadhaknath hen serum on egg quality
Parameter i j k l
“Weight of the egg (gm) 38.23a ± 1.07 27.82b± 1.79 37.00a± 1.08 39.20a ±1.77
Width of the egg (mm) 37.35a ± 1.65 37.05a± 1.42 35.40a± 2.55 32.90a ± 2.49
Length of the egg (mm) 49.85a ± 2.17 43.80a ± 1.09 44.10a±1.78 46.75a ± 3.90
Height of the thick albumin
(mm)
3.55a ± 0.401 3.61a ± 0.42 4.10a± 0.70 3.90a ± 0.42
Diameter of the albumin
(mm)
85.00a ± 3.64 73.85b ± 1.09 76.10b ± 2.16 70.10b ± 3.57
Height of the yolk (mm) 12.72a ± .83 12.65a ± 1.09 11.35a ± 1.08 12.75a ± 1.08
Width of the yolk (mm) 43.10a± 2.56 43.50a ± 2.17 36.75a ± 3.19 42.45a ± 1.44
Shell thickness (mm)” 0.30a ± 0.023 0.28a ± 0.04 0.25a± 0.02 0.33a± 0.04
Fresh yolk wt (gm) 13.86a ± 1.09 12.30a ± 1.09 12.75a± 0.71 13.65a ± 1.09
Fresh albumin wt (gm 18.74a ± 1.49 16.67a ± 0.72 16.95a± 1.47 17.02a ± 1.49
Shell dry wt (gm) 5.63a ± 0.72 5.85a ± 0.58 5.50a ± 0.41 6.02a ± 0.72
Different superscripts values in same column are significant statistically (P<0.05).

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[834]
Table 4. Effect of bromocriptine and serum from laying kadhaknath hen on hematological parameters.
Groups
TEC (million/mm3)
(Mean ± SEM)
Hb (gm/dl)
(Mean
± SEM)
PCV (%) (Mean)
±
SEM)
ESR (mm/1st hour)
(Mean
± SEM)
i 3.045a ± 0.600 10.30a ± 0.687 30.55a ± 0.126 5.235a ± 0.346
j 2.997a ± 0.400 10.68a ± 0.675 30.37a ± 0.929 5.839a ± 0.109
k 2.994a ± 0.60 10.63a ± 0.643 30.30a ± 0.516 5.894a ± 0.201
l 2.935a ± 0.090 10.07a ± 0.687 30.40a ± 0.109 5.975a ± 0.535
Different superscripts values in same column are significant statistically (P<0.05).
IV. CONCLUSION
This research is conducted for studying the impacts of anti-prolactin drug (Bromocriptine), and serum from
laying hen on egg production, pause days, prolactin level, egg quality, hematological values in indigenous
chickens. It is concluded that both Bromocriptine and serum of laying hen caused significant increase in
production of eggs and pause days decrease and in prolactin level. There was no significant difference (p>0.05)
noticed for the hematological values and internal and external qualities of eggs in birds of either treated groups
and un-treated control group. From the present study, it was concluded that combination of supplements of
Bromocriptine and serum of laying kadhaknath hen per day per birds was beneficial to increase the production
of eggs with no potential hazards to the indigenous chickens and these formulations can be used for increasing
production of eggs for our indigenous chicken.
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