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This document provides product information for a DNA Logic-Ladder sold by Bio Basic Inc. The DNA Logic-Ladder contains 16 DNA bands ranging from 100 base pairs to 10 kilobases that are formulated so that the amount of DNA in each band correlates logically to its size. This makes it useful for estimating DNA concentration for applications requiring consideration of DNA amount, such as probe labeling or optimizing ligation reactions. The ladder is tested to ensure the bands between 100 bp and 10 kb are distinguishable on a gel and that incubation overnight does not cause visible degradation.
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DNA Libraries / Genomic DNA vs cDNA .pdf
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For more details visit on YouTube; @SELF-EXPLANATORY; https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos Thanks...!
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Info.m103 r v2.0
- 1. Bio Basic Inc. QF 24 v2.0, 11/2011 20 Konrad Cres. Markham Ontario L3R 8T4 Canada Tel: (905) 474 4493, (800) 313 7224 Fax: (905) 474 5794 Email: order@biobasic.com Web: www.biobasic.com A world leader in serving science Product Information Catalogue Number: M103R-1 / M103R-2 Product Name: DNA Logic-Ladder (Ready-to-use) Size: 600µl / 5x600ul Description: The DNA Logic-Ladder contains 16 DNA bands ranging from 100 base pair (bp) to 10 kilobase (kp). The DNA Logic-Ladder was formulated to yield such a pattern that each band contains a LOGIC amount of the DNA fragment that correlates to its size. Thus, to measure both the size and the quantity of a particular DNA fragment could be achieved at a glance. It is particularly useful to estimate DNA concentration, such as for probe labeling, DNA sequencing, optimizing insert/vector ratio in ligation reactions and many other applications, which DNA concentration has to be taken into consideration. Recommended Loading: 6µl Storage Buffer: 10mM Tris-HCl, pH 8.0. 1mM EDTA. 1 x loading buffer: (5% Glycerol, 0.02% bromophenol blue, 0.02% xylene cyanol). Concentration: 920ng/6µl Storage: -20°C 0.9% agarose gel Quality Control: a) Agarose gel analysis shows that the bands between 100 bp and 10 kb are distinguishable. b) Incubation of 6µl of the DNA Logic-Ladder in restriction enzyme buffer overnight at 37°C shows absence of visible degradation on an ethidium bromide-stained agarose gel.