Eyes Infection 2017, Dr.Babiker Saad Almugadam Yousif, University of El Imam El Mahdi, Kosti city, Sudan

1. Eyes infection
Almugadam B Saad
Full name
Babiker Saad Almugadam YousifLecturer of medical microbiology
Faculty of medical laboratory sciencesFaculty of medical laboratory sciences
University of El Imam El Mahadi
Kosti-Sudan
MSc University of Khartoum
BSc University of El Imam El Mahadi

2. Eyes infection
Learning objectives:
-Anatomy
-Function-Function
Eye -Defense mechanism
-Normal flora
-Microbial infection
-Lab diagnosis

3. Anatomy of Eyes
internal -External part include Eye lid ,conjunctiva,
and external part cornea
-Internal part include uveal tract , Retina

5. Function of Eye
-organ of visions
Defense mechanism
-Include :
Eye lashesEye lashes
Normal flora
Tears
Lysozymes
Secretary IgA

6. Normal flora
-Include :
S.epidermidis
Nonpathogenic nesseria
Morxella spp
S.viridansS.viridans
diphtroid
Lactobacilli

7. Eyes infection ( Microbial infection)
-Classify to :
A- periocular infection (Infection of external
structure ) : which include conjunctivitis,Keratitis,
Blepharitis , Dacryoadenitis , Dacryocystitis , Canalic-
ulitis , Presepal and Orbital cellulitis.
B- Endophthalalmitis (Infection of internal
structure ): which include Uveitis , Scleritis ,Retinitis
and Chorionitis. cause mainly by protozoa , also
by bacteria and virus

8. Periocular infection
- Blepharitis : inflammation of eye lids . cause by S.auerus , S.epider-
midis , M.lacunata , C.albican and Some HSV.
- Keratitis : inflammation of cornea , ulcer form may lead to blindn-
ess. Mainly cause by bacteria or fungi , rarely by free living amoeba
(Acanthamaeba) .
Bacteria include : C.trachomatis (Trachoma) , S.auerus ,S.pnumonia
and P.aeruginosa.
Fungi include : Fusarium Spp and Aspergillus
-conjunctivitis :inflammation of conjunctiva ,can be acute or chronic .
Also can associated with infection of eye lid (Blepharoconj- unctivitis)
or cornea (Keratoconjunctivitis).

9. It mainly cause by bacteria. Also cause by Virus , Protozoa
and Allergic reaction.
Bacteria conjunctivitis cause by S.auerus , S.pnumonia,
H.influnzae , H.aegyptius ,C.trachomatis and P.aeruginosa
spp. Less commonly by Enterobacteriace.
Viral conjunctivitis cause by adenovirus , HSV and Coxsakivirus.Viral conjunctivitis cause by adenovirus , HSV and Coxsakivirus.
Neonatal conjunctivitis (Ophthalma neonatarum) :
cause commonly by N.gonorrhea , C.trachomatis (D-K) and
S.agalatciae . Also cause by S.auerus , S.pnumonia , S.haemolyticus,
and HSV.

10. Lab diagnosis of eye infection
-Specimen : Eye swab (2), and scraping in case of keratitis
-Transportation : stuart or Amies transport media
-Microexaminatio: Gram stain
Direct immunofluorescence stain
In case of Keratitis Geimsa stain
Iodine preparation
-Culture :-Culture :
Routine : BA , Chochlate agar and McConkey agar if
enterobacteriace suspect.
Additive media :
cell culture → in Keratitis or if virus infection suspect
Sabroad → if fungi suspect
An O2 BA
Media for fungi Serious eye
Liquid media (thioglycolate broth) infection