Introduction part of research paper

1. Molecular characterization
of human ABHD2 as TAG
lipase and ester hydrolase
Babanjeet
L-2021-H-85-D
Naresh et al 2016

2. Lipases
 Supply of energy
 Lipid signalling
 Metabolism
Aberrations
cause many human diseases
like cancer
α/β hydrolase domain (ABHD)
Novel potential regulators
 lipid metabolism
 Signal transduction
ABHD
Family contains 21 proteins
Amino acid residue
ABHD proteins also possess
another conserved motif HXXXXD
Motif
The motif was attributed to
acyltransferase activity
L
A
A
A
A
M

3. ABHD
Hydrolase activity of ABHD is
attributed to the catalytic triad
composed of serine-acid-histidine
residues located in loop regions
The acid residue of the catalytic triad
can either be a glutamate or aspartate,
usually located after strand β7
Structure of α/β hydrolase domain

4. Proteins Significant role References
ABHD3 Cleaved medium chain phospholipids Simon et al 2006
ABHD4 Lyso-N-acyl phosphatidylethanolamine Brady et al 2011
ABHD5 Human Chanarin Dorfman Syndrome Lefervre et al 2001
ABHD6 Neurotransmission Marrs et al 2010
ABHD12 Usher Syndrome Savinainen et al 2012
ABHD16A Hydro lysing unsaturated monoacylglycerols (MAGs) Eisenberger et al 2012
ABHD2 Atherosclerosis and emphysema involving macrophage recruitment Edgar et al 2002
Characterization of ABHD proteins

5. THANK YOU!