Discussion of papers

6. Floral Biology
Okra flowers are 4-8 cm in diameter, with
five white to yellow petals, often with a red
or purple spot at the base of each petal.
Flower bud appears in the axil of each
leaf, above 6th to 8th leaf.
A flower bud takes about 22-26 days from
initiation to full bloom.

9.  The major problem in the vegetable production is
the heavy incidences of diseases and pests.
 Host plant resistance is the economical method
but such resistance against insect pest is never
stable due to population pressure of insects on
the host, as a result there is evolution of new
biotypes and breakdown of resistance.
 Insect-pests are responsible for reducing about
40% yield in vegetables
Interspecific Hybridization of C. Annuum with Wild Species.

10. Disease Markers associated
Potyvirus resistance pvr1, pvr2, pvr5, pvr6, Pvr4 and Pvr7
Cucumber mosaic virus cmv11.1, cmv12.1
Tabacco mosaic virus L. L1, L4
Tomato spotted wilt virus Tsw
Phytophthora resistance Phy5.2
Powdery mildew Lt_6.1
Colletotrichum sp. B1
Bacterial spot Bs1, Bs2, Bs3 and Bs4
Root knot nematodes Me3, Me4, N

12. Rootstocks for controlled diseases and pests of various
vegetables by grafting.

13.  Plant material: Inbred line OHB3-1
 For RAD-seq analysis, two parental bitter gourd
lines, OHB61-5 and OHB95-1A, and their F2
progeny were used as materials.
Expression analysis of orthologous
genes for sex determination in bitter
gourd.
CmAcs11
CmAcs7
Cmwip1

14.  Sequence assembly Sequence
 Gene prediction and annotation
 Comparative analysis of genomes among Cucurbitaceae
 Conserved genes among Cucurbitaceae species and unique genes
 RAD-seq analysis
 Reference mapping of RAD-seq tags
 Linkage map development
 Comparative analysis of orthologous and paralogous genes
MiSeq and HiSeq 2500 system
FGENESH software
SyMap 4.2
Aminosan and RAxML
HiSeq2500 system
BWA version 0.6.1 in DDBJ
JoinMap
MEGA7.0.18

15. Sequence reads of bitter gourd (OHB3-1) genome for de novo
assembly
Summary of assembly results in OHB3-1 genome sequence
Phylogenetic relationship among Cucurbitaceae species
Summary of predicted genes in bitter gourd (OHB3-1) scaffold
sequence

16. Reference sequences (OHB3-1) mapping results of RAD-seq
tags of OHB95-1A and OHB61-5
Summary of synteny mapping of bitter gourd scaffolds to the
genome sequence of cucumber, melon, and watermelon Phylogenic analysis of putative trypsin inhibitor and
ribosome inactivating protein in bitter gourd.

17. Phylogenic analysis of sex determination related genes in
Cucurbitaceae
Number of predicted genes encoding trypsin-inhibitor
and ribosome inactivating protein in cucurbits.

18. Thank You!