This PPT is sharing with you how to process urine sample in laboratories. This PPT also included right way to make proper diagnosis. It will be very useful for laboratory technicians to understand that what is correct way to process the urine sample. Step by steps processing also accepting criteria of Kass technique to report the pathogenic organism in urine sample.

1. URINE SAMPLE COLLECTION
AND
PROCESSING IN MICROBIOLOGY LAB
ARPITA CHANDRA

2. SPECIMEN COLLECTION
1. Midstream urine (for culture and AST )
Sterile Wide mouthed container
Before collection
Genitalia should be properly cleaned
With soap & water
Then
Mid portion of the stream is collected

3. 2. CATHETER SPECIMEN
Collect directly from catherter tube not from catheter bag.
3. From infants
Suprapubic Aspiration
TRANSPORT
Process with minimum delay
If not possible – Refrigerated at 40°C or stored by adding
boric Acid.

4. PHYSICAL EXAMINATION
- COLOUR
- VOLUME
- ODOR
- SPECIFIC GRAVITY
- PRESENCE OF BLOOD

5. After Centrifugation of urine sample
Supernatant Sediment
Chemical analysis
1. pH
2. Glucose
3. Protein
4. Ketone bodies
5. Urobilinogen
6. Bilirubin
7. Nitrite
8. Bile salts, Bile pigments
9. Blood
Wet mount preparation
(examined under 40X)
1. Pus cells
2. Crystals
3. Microorganism
4. Parasites
5. BYC
6. Contamination

7. CULTURE FROM SEDIMENT PART
STREAK ON
CLED (Primarily used for urinary pathogens)
Also Can be used
 Nutrient agar
 Blood agar
 Mac-Conkey

8. AFTER INCUBATION OF 24 HR’S AT 35°C
Observe colony morphology from culture plates
Marked isolated colonies for smear
Smear preparation and gram stain
(examined under oil immersion-100X)
GPC GNB
Gram positive cocci Gram Negative bacilli

10. COLONY COUNT BY KASS TECHNIQUE
(1956)
Total Bacterial count per ml = Number of colonies x 200
Number of colonies = No. of Bacteria present
INTERPRETATION OF RESULT
1. >105 Bacteria/ml = Significant Bacteriuria(Active UTI)
2. Between 104 to 105 Bacteria/ml = doubtful Significance
(Repeated culture)
3. <104 Bacteria/ml = No significance growth
(more than 3 type= regarded as contamination)