BACTERIOLOGY ANALYSIS OF WATER AND Water Sampling.pptx
1. Water sampling and Bacteriological
analysis of drinking water.
Dr.Mittal Rathod,
Community Medicine Dept.,
Associate Professor
AIIMS, JAMMU.
2. Contents
โข Safe & wholesome water
โข Water Quality โCriteria & Standards
โข Water Sampling
โข Bacteriological Analysis of Water: Methods
โข Public Health significance
3. Learning Objectives
โข To understand the water sampling methods.
โข To know various Bacteriological indicators .
โข To describe its importance & its usage.
4. Safe And Wholesome water
1. Pleasant to taste
2. Free from Pathogens
3. Free from chemicals
4. Usable for domestic purposes
5. Water Quality โCriteria & Standards
1. Acceptability aspects
a. Physical Parameters
b. Inorganic constituents
2. Microbiological aspects
a. Bacteriological Indicators
b. Virological aspects
c. Biological aspects
3.Chemical aspects
4. Radiological aspects
7. Water Sampling
1. Sampling for Physical & Chemical
Examination:
2. Sampling for Bacteriological
Examination:
-
8. Sampling for Physical & Chemical
Examination:
โข Clean glass stoppered bottles
โWinchester Quart Bottlesโ
โข Capacity Not < 2 L.
โข Precautions:
9. Sampling for Bacteriological
Examination:
โข General Considerations:
๏ผClean sterilized glass bottle with glass stopper
๏ผCapacity: 200-250 ml
๏ผIf sampled water is clorinated- add small
quantity of SODIUM THIOSULPHATE.
๏ผAfter collection immediately send to PHL.
10. Collection of the Sample From A Tap
โข Open the tap fully
โข Allow to run the water for at least 2
minutes
โข Fill the sample bottle from gentle
stream of water, avoid splashing.
โข If tap is required to be sterilized than
use blow lamp/ spirit lamp
11. Collection of the Sample From Rivers,
Lakes, Reservoirs, Wells
โข Not to far or not too near the
bank.
โข Take sample from Point of draw
off.
โข Here bottle requires string.
โข Not too superficial or not too
deep
12. Transport & Storage of samples
โข As soon as possible.
โข If required to store: keep in ice, not more than
48 hrs.
โข Write date & time of collection, dispatch,
source of water, any recent rainfall, sanitary
survey details.
17. Reasons
1. Abendance of coliforms constantly in human
intestine.
2. Easily detected
3. Longer survival
4. Have greater resistance to forces of natural
purification
18. Methods for detection of Coliforms
โข Presumptive coliform test
a. Multiple tube method
โข Confirmatory Test: EIKJMANโS TEST
โข Membrane Filtration Technique
โข Colony count
19. Multiple tube method
โข Estimation of MPN of coliform organisms in
100 ml of water.
โข Culture Media: McConkeyโs Lactose Bile Salt
Broth.
โข Indicator: Bromocresol Purple
Incubate for 48 hrs
20. MPN contโฆ
โข Results: Fermentation & Acid & Gas formation
โข โ Presumptionโ of coliform organisms
21. Confirmatory Test
โข Subculture each presumptive positive tubes in
2 tubes of brilliant green bile broth.
One tube at 37 C * 48 hrs
Second tube at 44 C *6-24 hrs
True MPN Index: McCradyโs tables.
22.
23. Most Probable Number (MPN)
# Positive
Tubes
MPN Index
(CFU/100mL)
0 <1.1
1 1.1
2 2.2
3 3.6
4 5.1
5 6.9
6 9.2
7 12.0
8 16.1
9 23.0
10 >23.0
Sample Table for
10 tube test
24. Most Probable Number (MPN)
โ Quantitative results
โ Simple to understand and use
โ Relatively inexpensive
โ Can be used with turbid water
โ Requires some training & Requires incubator &
other equipment
25. Membrane Filtration Technique
โข A measured volumn of water is filtered
through a membrane โ Cellulose esterโ.
โข Bacteria present in water- Retained on the
surface of the membrane.
โข Inoculate on media
โข Count the colonies
26.
27. 25
Membrane Filtration: Advatages
โMost accurate test method
โAbility to test many samples at once
โInternationally recognized method
โRapid, Gives direct result.
โUseful in rural areas,Samples can be tested in
the field
28. 26
Membrane Filtration: Limitations
โ Requires more training
โ Requires additional equipment
โ Cost of consumables can be high
โ Turbid water interferes with bacterial growth.
โ Noncoliforms interferes with counting of coliforms.
โ Toxic substances in the water may be absorbed by
filter and interferes with bacterial growth.
30. Colony count contโฆ
โข On Nutrient Agar:
37 deg C 22 deg C
โข Count the colonies
โข Sudden increase in colony count: earliest
indication of contamination.
31. โข Recommended plate count:
Water at point
of consumption
After 2 days at
37 deg C
After 3 days at
22 deg C
Disinfected 0 20
Not- disinfected 10 100
32.
33. Faecal steptococci
โข It occurs regularly in Faeces but lesser than E.coli.
โข It is a indicator of Recent fecal contamination.
โข Highly resistant to drying.
โข Imp: after laying new mains or repairs in
distribution systems/ surface run off/ ground
water.
34. Cl. perfringens
โข It occurs regularly in Faeces but lesser than
E.coli.
โข Spores- survive in water for longer duration.
โข It is a indicator of Remote fecal contamination,
in absence of coliform.