This document describes the process of isolating and identifying Candida albicans from urine samples. Midstream urine samples were collected in sterile containers and sent to the laboratory for analysis. The samples were cultured on Sabouraud dextrose agar plates containing chloramphenicol and incubated at 37°C to examine for fungal growth. Colonies suspected to be Candida species were identified microscopically using gram stain and lactophenol cotton blue stain. Identification of C. albicans was confirmed using a germ tube test, where growth in human serum at 37°C results in the formation of long tube-like projections called germ tubes.

1. Isolation of Candida albicans from urine
Sample Collection
1-Midstream urine samples with wide
mouthed sterile capped containers after
proper cleaning of the external genitalia.
2-The urine samples were labeled and
immediately sent to the laboratory for
analysis

2. Candida spp
normal flora of human body in gastrointestinal
tract, vagina, oral cavity, skin and mucosal
surfaces of human
• Candida albicans is an opportunistic fungal
pathogen estimated to colonize the
urinogenital tracts of 30% of women (Achkar
and Fries, 2010).
• Sabouraud Dextrose Agar(SDA) with 75 mg/L
chloramphenicol

3. SDA
• Composition of SDA
• Ingredients In gm/L
• Dextrose (Glucose) 40 gm
• Peptone 10 gm
• Agar 15 gm
• Distilled Water 1000 ml

4. Isolation Candida spp.
Urine Samples were cultured on Sabourauds dextrose
agar plates, containing 0.5 mg per 1000ml
chloramphenicol and incubated at 37 0C and examined
for its growth at 24, 48 and 72h.

5. Microscopically identification of Candida spp.
Gram stain to identified Candida spp and use lacto
phenol cotton blue to stain Candida finally
examined under 40 x and 100 x with oil emersion.

6. Germ tube test for identification of Candida albicans
Colony inoculums of Candida cultures was inoculated
into 0.5 ml of human serum in a test tube and
incubated at 37 0C for 2-4 hours. After incubation, a
loop-full of culture was placed on a glass slide, overlaid
with a cover-slip and examined microscopically for the
presence or absence of germ tube

7. • Formation of long tube like projections
extending from the yeast cells with no
constriction or septa .The germ tube is
indicative of C. albicans.
• Candida albicans white colored, smooth, and
yeast-like in appearance.
• spherical to sub spherical, and Hyphae or
pseudohyphae

8. Candida culture

9. Germ tube of C.albicans