Quantifying Artificial Intelligence and What Comes Next!
KBIPER.Ocimum.pdf
1. PHYTOCHEMICALAND BIOLOGICAL
EVALUATION OF GREEN AND BLACK
OCIMUM SANCTUM
Shree Joshi, Anshu Srivastava, Milind Bagul, Avani Kothari and Neeta Shrivastava
B. V. Patel Pharmaceutical Education and Research Development (PERD) Centre,
Thaltej, Ahmedabad – 380 054
Phone: 079/ 27439375, Website: www.perdcentre.com
2. THE PLANT
Ocimum sanctum (Lamiaceae)
Chemical constituents
Major: Volatile oil (consists eugenol - 70.5%, nerol,
caryophyllene camphene, etc.)
Minor: Alkaloids, glycosides, saponins and tannins
Therapeutic uses
Classical: Indigestion, cough, malarial fever, wound, poisoning,
eye diseases, etc.
Scientific: Antitubercular, antiulcerogenic, antifertility,
anticancer, antiasthmatic, hepatoprotective, immunomodulatory,
etc.
6. Parameters
ESTIMATION OF TOTAL PHENOLICS
(Singleton and Rossi method, 1965)
ESTIMATION OF VOLATILE OIL
(Hydrodistillation method)
ESTIMATION OF MARKER COMPOUND
EUGENOL
(HPTLC method)
7. Parameters Green Tulsi Black Tulsi
Total phenolic
content
0.65% 1.75%
Volatile oil 2.0% 1.6%
Eugenol 0.4% 0.6%
Results
8. Chromatogram for Estimation of Eugenol
Mobile phase - Toluene: Ethyl Acetate: Formic Acid (6: 4: 0.4)
Eugenol
BT
GT
9. TLC FINGERPRINTING
1) Test sample
Volatile oil and Methanolic extract
2) Mobile phase
Volatile oil with Eugenol
Toluene: Ethyl Acetate (8.5: 1.5)
Methanolic extract
Toluene: Ethyl Acetate: Formic acid (6: 4: 0.4)
10. Fingerprinting of Volatile Oil with Eugenol
Rf VALUE OF BANDS
GT STANDARD (EUGENOL) BT
0.56 - -
0.67 0.67 0.67
0.77 - -
0.97 - 0.97
BT
EUGENOL
GT
11. Fingerprinting of Methanolic Extract
TLC Fingerprinting after derivatization
GT BT
Color of bands Rf value
GT BT
Violet 0.07 0.07
Violet 0.12 0.12
Light blue 0.16 0.16
Light blue 0.33 0.33
Dark blue 0.52 0.52
Dark green 0.59 0.59
Purple 0.70 0.70
Dark green 0.73 0.73
Pink 0.81 0.81
Violet 0.95 0.95
Violet 0.98 0.98
13. FREE RADICAL SCAVENGING ACTIVITY
DPPH MODEL
1) Test sample – Methanolic extract
2) Stock solution - DPPH in methanol
3) Reaction mixture
2 ml 100 l extract in different
+ +
methanol DPPH concentrations
Incubation for
15 mins.
Absorbance recorded at 516 nm
15. ANTIMICROBIAL ACTIVITY
1) Test sample
Volatile oil and petroleum ether extract
2) Microorganisms
Eleven bacterial ATCC cultures
3) Medium
Antibiotic assay medium No. 1
4) Positive control
Ciprofloxacin: 4 g/ ml
AGAR PLATE METHOD
19. SUMMARY
● Phytochemical profile and Biological activity
of Green and Black Tulsi are considerably
different
● Volatile oil content is more in Green Tulsi
while total phenolic content and eugenol
content is higher in Black Tulsi
● TLC fingerprinting profile of Volatile oil of
both the types of Tulsi showed variation
● Biological activity showed higher potential in
Black Tulsi
20. CONCLUSION
Black Tulsi showed higher potential in Biological
activity than Green Tulsi that may be attributed to
the High Eugenol and Total phenolic content
The study scientifically supports the belief that Black Tulsi is
medicinally more valuable than Green Tulsi