ELECTRON MICROSCOPY

1. Electron Microscopy
Transmission Electron Microscope (TEM)
&
Scanning Electron Microscope (SEM)
Ankush R. Kayarkar
M.Sc. Botany (Gold Medalist)
Research Student
Post Graduate Department of Botany
RTM Nagpur University, Nagpur 440033
Email- ankushkayarkar92@gmail.com

2. Why Electron Microscope?
Ø
Light Microscopes are limited by the physics of light
to 500x or 1000x magnification and a resolution of
0.2 micrometers.
Ø
In the early 1930's there was a scientific desire to see
the fine details of the interior structures of organic
cells (nucleus, mitochondria...etc.).
Ø
This required 10,000x plus magnification which was
just not possible using Light Microscopes.

4. Comparison Between Light & Electron Microscope:

15. SCANNING ELECTRON MICROSCOPE
(SEM)
A scanning electron microscope (SEM) is a type of electron
microscope that images a sample by scanning it with a high
energy beam of electrons in a scan pattern. The electrons
interact with the atoms that make up the sample producing
signals that contain information about the sample's surface
topography, composition, and other properties.

27. Thank You…
Ankush R. Kayarkar
M.Sc. Botany (Gold Medalist)
Research Student
Post Graduate Department of Botany
RTM Nagpur University, Nagpur 440033
Email- ankushkayarkar92@gmail.com
Mob. No. 8806323234