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1. Southern Blotting Technique
Presented By :
Name: Anik Banik
ID. No – 1606034
Reg. No -3445
Faculty Of Biotechnology And Genetic Engineering,
Sylhet Agricultural University,Sylhet-3100

2. WHAT is BLOTTING
• It’s the transfer of macromolecules like proteins
and nucleic acids from gel onto surface of
immobilized membrane similar to gel.
• These powerful techniques allow us to identify
and characterize specific molecules in a
complex mixture of related molecules.

3. TYPES
Blotting Technique
Southern Blot
It is used to detect DNA.
Northern Blot
It is used to detect RNA.
Western blot
It is used to detect protein.

4. SOUTHERN BLOTTING
• It is a method used for detection of a specific DNA
sequence in DNA samples.
• It combines transfer of electrophoresis-separated
DNA fragments to a filter membrane.
• Then subsequent fragment detection by probe
hybridization.

5. APPARATUS
Figure : Apparatus For Southern Blotting

6. STEPS
6
Digest DNA with
restriction endonuclease
Electrophoresis to separate
fragments
Blot fragments onto a
nitrocellulose
membrane Hybridize with
the 32Pprobe
Autoradiography

7. 7
SCHEMATIC REPRESENTATION
Figure: Schematic Representation of Southern Blotting

8. APPLICATION
• Diagnosis & detection of genetic diseases
• Used for paternity testing, criminal identification,
• To isolate and identify desire gene of interest.
• To identify mutation or gene rearrangement Used
to identify infectious agents.

9. ADVANTAGES
• Effective way to detect a specific DNA
sequence in a complex sampleof DNA.
• Can be used to quantify the amount of the
present DNA.
• Cheaper than DNAsequencing.

10. DISADVANTAGES
• More expansive than most other tests.
• Complex and labor-intensive.
• Time consuming.

11. THANK YOU FOR YOUR
KIND ATTENTION !