It describes a modern day methodology that is frequently used to produce novel products and improve the quality of products effectively. This PPT includes only a few of the methods that have been discovered. Kindly inform if any corrections or inclusions are needed. And yes, suggestions are always heartily welcome.

1. Transgenesis
Presented By :-
Anand Mohan Hasda
(217BM2371)
National Institute Of Technology, Rourkela

2. Nothing to be surprised about…..

3. Contents
What is Transgenesis?
Methods prior to Transgenesis
Uses & Examples Of Transgenesis
Next Gen Products in the Pipeline
Methods Of Transgenesis
Implications Of Transgenesis

4. What is
transgenesis?
It is the process of introducing an exogenous
gene (transgene) into a new living organism
so that the organism will exhibit the new
property and transmit the same to its offspring.
Transgene - The newly introduced gene
sequence or DNA.

5. Methods prior to Transgenesis
In both animals and plants:
• Selective breeding
• In breeding
• Out breeding
In plants only:
• Vegetative propagation
• Grafting

6. Uses Of
Transgenesis• Toxicology – Detection of toxicants
• In humans, for disease detection
• In molecular biology, for analysis of regulation of gene expression
• In developing organisms, especially for xenografting i.e. to modify
the antigenic makeup of an organism so that their tissues and organs
can be used in transfusions and transplants.
• To genetically engineer hormones to aid in increase of milk yield,
meat production; genetically engineer livestock to study the
modification in animal physiology and/or anatomy.
• To speed up the introduction of existing characters into a
strain/breed for improvement and modification.
• In pharmaceutical industry, for specific pharmaceutical protein
production, drug production, and drug efficacy testing.

7. Examples Of
Transgenesis
• Flavr Savr Tomato
• Herbicide resistant soyabean
• Bt Brinjal, Bt Cotton
• bST (Bovine Somatotropin)
• Chymosin

8. Next Gen Products in
the Pipeline
• Golden Rice
• Turfgrass
• Biosteel
• Decaffeinated coffee
• Disease resistant bananas, cassava, sweet potatoes
etc.
• Bananas to deliver Hepatitis vaccine
• Apples for protection against RSV (Respiratory
Syncytial Virus)

9. Methods for
Transgenesis• Genetic Engineering (r-DNA technology)*
• Microinjection
• Embryonic stem cell mediated transfer
• Retrovirus mediated gene transfer
• Agrobacterium mediated gene transfer
• Gene gun (Biolistics)
NOTE:
*First and most important step

10. Genetic Engineering

11. Microinjection

12. Embryonic stem cell-mediated transfer

13. Retrovirus mediated gene transfer

14. Retrovirus mediated gene transfer

15. Agrobacterium mediated gene transfer

16. Gene gun (Biolistics)

17. Implications Of
TransgenesisPositive
• Many valuable pharmaceutical
products such as clotting
factors, human protein
coagulant, α-1 antitrypsin for
deficiency treatment, insulin
for diabetes treatment,
growth hormones for various
deficiencies etc.
• Treat human diseases and
defects
• Improving plants
• Improving animals
Negative
• Unpredictability
• Biosafety concerns
• Ethical issues
• Unintended effects
• Allergenicity
• Toxicity

18. Thank You!