Biopesticide (2).pptx .This slides helps to know the different types of biop...
The Structure and Mechanism of Bacterial Cell Wall Destruction by Lysozyme
1.
2. Introduction
Lysozyme is also called as muramidase or
N-acetylmuramide glycohydrolase.
It is antimicrobial enzyme and small
globular protein, and present in tears,
nasal mucous, gastric secretion, milk and
egg white.
It lyse the bacterial cell wall.
Turn over no. : 0.5/sec. and E.C. no is
3.2.1.17
In 1922, Alexander Fleming coined the
term lysozyme.
3. Structure of Lysozyme
In 1965, David Phillips and his
colleague’s determined 3D structure by X-
ray crystallography.
Molecular weight : 14.3 Kda
Shape is ellipsoidal and dimension are
45X30X30 Ao
It contain polypeptide chain of 129 amino
acid
Lysin at the N terminal and Leucine at the
C terminal.
4.
5. Structure of Lysozyme
Active site of lysozyme are of six subunit
which are bind to six substrate molecules.
6. MECHANISM OF ACTION OF
LYSOZYME
Glutamate-35 and Aspartate-52 are
present at active site. After binding, D
residue is distorted half-chair conformation.
Glu-35 transfer its proton to oxygen and
break the bond and generate carbonium
ion.
Asp-52 stablize that ion , E ring will
released.
Followed by addition of water, enzyme will
regenerated and ring D will be released.