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Biosynthesis of amino acids

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Biosynthesis of amino acids

  1. 1. Biosynthesis of Amino Acids M.Prasad Naidu MSc Medical Biochemistry, Ph.D,.
  2. 2. Overview
  3. 3. Overview (cont)
  4. 4. Biosynthetic Families Metabolic Precursors Amino Acids -ketoglutarate Glutamate Glutamine Proline Arginine 3-Phophoglycerate Serine Glycine Cysteine
  5. 5. Biosynthetic Families (cont) Metabolic Precursors Amino Acids Oxaloacetate Aspartate, Asparagine Methionine, Threonine, Lysine Pyruvate Alanine, Valine, Leucine Isoleucine
  6. 6. Biosynthetic Families (cont) Metabolic Precursors Amino Acids Phosphoenolpyruvate Tryptophan and erythrose 4-phosphate Phenlyalanine Tyrosine Ribose 5-phophate Histidine
  7. 7. Ketoglutarate Family Ketoglutarate Glutamate Glutamine Proline Arginine
  8. 8. -Ketoglutamate to glutamate/glutamine Glutamate + NH4 + + ATP Glutamine synthetase Glutamine + ADP + Pi + H+ -Ketoglutamate + glutamine + NADPH + H+ Glutamate synthase 2 glutamate + NADP+
  9. 9. -Ketoglutamate to glutamate/glutamine Net Reaction: -Ketoglutarate + NH4 + + NADPH + ATP L-glutamate + NADP+ + ADP + Pi
  10. 10. Ketoglutarate Family Ketoglutarate Glutamate Glutamine Proline Arginine
  11. 11. Synthesis of Proline
  12. 12. Synthesis of Proline
  13. 13. Synthesis of Arginine
  14. 14. Synthesis of Arginine
  15. 15. 3-Phosphoglycerate Family 3-Phosphoglycerate Serine Glycine Cysteine
  16. 16. Synthesis of Serine and Glycine
  17. 17. Synthesis of Cysteine
  18. 18. Oxoloacetate Family Oxaloacetate Aspartate Asparagine Methionine Lysine Threonine
  19. 19. Synthesis of Aspartate and Asparagine Oxaloacetate AspartateTransamination from glutamate Amidation with Glutamine donating NH4 + Asparagine
  20. 20. Synthesis of Lysine, Methionine, and Threonine • See handout
  21. 21. Pyruvate Family Pyruvate Alanine Valine Leucine Isoleucine
  22. 22. Synthesis of Alanine Pyruvate AlanineTransamination from glutamate
  23. 23. Synthesis of Isoleucine, Valine,and Leucine • See handout
  24. 24. Phosphoenolpyruvate and erythrose 4-phosphate Family Phosphoenolpyruvate + erythrose 4-phosphate Chorismate Tryptophan Tyrosine Phenylalanine
  25. 25. Synthesis of Chorismate
  26. 26. Synthesis of Tryptophan
  27. 27. Synthesis of Tyrosine and Phenylalanine
  28. 28. Synthesis of Tyrosine and Phenylalanine (cont)
  29. 29. Biosynthesis of Histidine • See handout
  30. 30. Possible Test Questions 1) Provide the 6 metabolic precursors and the amino acids which they lead to. 2) Duplicate the Amino Acid Synthesis Overview Flow Chart. Expand the sections need to show the synthesis of Glutamate, Glutamine, Proline, Arginine, Serine, Cysteine, Glycine, and starting from Chorismate Tryptophan, Tyrosine and Phenylalanine. (40 points!!)

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