Gene Silencing at RNA Level Two methods are discussed in this ppt i.e. RNAi and CRISPR

1. Inhibition of Gene Expression at
RNA Level
Presenter: Ahmed Madni
MS Research Scholar (Biotechnology)

2. List of Contents
 Introduction
 Techniques employed
 Generation & Mechanism of miRNA
 Generation & Mechanism of siRNA
 CRISPR
 RNAi Vs CRISPR
 Current Status & Future Prospectus
6/26/2018 2

3. Gene Expression
6/26/2018 3

4. Gene Expression
X X X
6/26/2018 4

5. Techniques used to Silence Gene
6/26/2018
CRISPRRNA Interference (RNAi)
5

6. RNA Interference (RNAi)
6/26/2018 6

7. RNA Interference (RNAi)
For precise targeting to silence gene
 Gene Control
 Tool for Research
 Therapy
6/26/2018 7

8. Generation of miRNA
RNA Polymerase II
Drosha
DGCR8
Miroprocessor
Complex
Dicer
Pre-miRNA
6/26/2018
Pri-miRNA
8

9. Mechanism of miRNA
TRNC6
6/26/2018
miRNA:miRNA Duplex Complex
Argonaute
Guide RNA
+ Argonaute
9

10. Generation & Mechanism of siRNA
6/26/2018
Dicer
Argonaute
Piwi Domain
Guide RNA + Argonaute
10

11. CRISPR
Neisseria meningitidis CRISPR-Cas9
https://www.sciencedaily.com/releases/2018/02/180215141724.htm
6/26/2018 11

12. RNAi Vs CRISPR
6/26/2018 12

13. Current Status & Future Prospectus
6/26/2018
Source: Google Trends 13

14. 6/26/2018 14