Genetically Modified
      Foods
       By: Emily Luti
How does it work?
 Genes use messages to make certain enzymes.
 By introducing a foreign gene, new enzymes are
 made according to that gene.




                          http://www.youtube.com/watch?v=RoCII
                          huLl8A&feature=related&safety_mode=tr
                          ue&persist_safety_mode=1&safe=active
Techniques


 Bacterial Carriers:
    Bacterium such as Agrobacterium,
       is able to transfer DNA well to plants.
        Bacterium prepared in special solution to make cell walls more
         permeable.
        Selected gene is placed in the extra-chromosomal DNA (plasmid) of other
         bacterium and dropped into solution.
        Solution is heated, giving the plasmid access to the Agrobacterium, and
         the gene begins to express itself.
        The genetically altered bacterium is allowed to grow, then infect plants for
         the gene to be expressed.
Techniques

 Biolistics
   Selected DNA is attached to small particles of either gold or
    metal tungsten.
   DNA particles are shot into the target cells using pressure.
Techniques

 Calcium Phosphate precipitation
   Selected DNA is exposed to calcium phosphate, tiny granules
      are created.
   Target cells respond to granules, and surround and digest
      them.
   This allows the granules to

  release the DNA and deliver
  it to the host Nuclei and its DNA.
Techniques

 Electroporation
   Prepared cells are surrounded by a special solution with the selected
     DNA.
   A short electric shock is passed through the solution.

   The result is a permeable cell wall for the DNA to enter the nuclei
     through.
   Cells are placed into another solution which encourages to repair of
     their cell walls, locking the foreign DNA within.
   The new DNA is incorporated

   into the chromosomes, and
  the host has a new gene.
Techniques

 Gene Silencing
   The gene that controls the undesirable trait is identified.

   A second copy of the gene, facing the wrong way around, is
    attached to the undesirable gene.
Techniques

 Gene Splicing
    Restriction enzymes can cut their DNA into “sticky” fragments and paste
     them directly to another set of DNA for infection.
    These restriction enzymes are used by scientists to genetically engineer
     cells. The DNA is cut, then inserted into a different set of DNA, where DNA
     ligase is used to fuse the new gene sequence to the chromosome.
    Alternatively, the gene may be placed into a bacterial plasmid and allowed to
     enter the defective cell and deliver the new gene.
Techniques

 Lipofection
   Small bubbles of fat, liposomes, are used to carry the specific
    DNA.
   The target cells and liposomes are put into a special solution.

   The liposomes combine with the phospholipids in the cell
    membrane, allowing the DNA to enter the cell and combine
    with the chromosomes.
Techniques

 Microinjection
   Selected DNA is inserted into a female ovarian egg through a
    glass capillary tube.
   The egg is transferred into the prepared uterus of a female to
    grow full term.
   Creates a transgenic animal

  that will have all new cells.
Techniques

 Viral Carriers
   Selected DNA is added to a virus.

   Virus is then allowed to infect the target.

   As the virus replicates inside the invaded cell, the DNA is
    added to the cells genetic make-up.
Industry

 Countries using:
   Japan

   Malaysia

   Australia

   Europe (pretty much everyone)

   USA

   Canada

   Most first world countries and some second world

    Most countries require labels for consumer
     choice
Examples?

 Plants:
  Rice
  Soybean

  Sugar Cane

  Tomatoes

  Corn

  Potatoes

  Peas

  Squash,ect.
Examples?

 Meats
   Cattle

   Pigs

 Other products
   Honey

   Cotton

   Oils

   Tobacco

   Dairy

   Vitamins
The Debate: Good or Bad?
 Advantages
     Medical Advances
         Edible Vaccines
     Virtual End to World Hunger
         No mal nutrition
         Grown in bulk/ plenty supply
     Cheaper/Faster to Grow
         Don’t have to be rich to plant
     Environmentally Friendly
         No pesticides
         No run-off into water supply
     Sustainable
         Flood/Drought tolerant
     Better Nutrition
         Vitamin enriched
     Better Bang for your Buck
         Grown bigger
     Endless possibilities
         Anything alive can be genetically modified
The Debate: Good or Bad?
 Disadvantages
     Opposition
         Religion
         Regulation/ money cost for approvement
     What will it do in the future?
         Health risks
         Allergies
         Help or not?
     Medicine
         Antibiotic resistance
     What could it create?
         Resistant weeds
     Pollination
         Cross pollination in wild
     Choice?
         Labeling/ Health approval in some countries
     Incidents already occurring
         1989 incident killed dozens with infected food supplement L-tryptophan
         2006 contaminated exported rice genes
Autobiography

 Butcher, Mavis. "Genetically Modified Food-GM Foods List and
        Information." Disabled-World.com. Disabled-World, 22 Sept. 2009.
        Web. 16 May 2012. <http://www.disabled-worl.com/fitness/gm-
       foods.php>.
 Deakin University- Faculty of Health and Behavioral Sciences. "Genetically
       Modified Foods-Techniques.“ Better Health Channel. State of
        Victoria. Web. 10 May 2012. <http://www.betterhealth.vic.gov.au
        /bhcv2/bhcarticles.nsf/pages/Genetically_modified_foods_techniques>.
 Fedoroff, Nina V. "Engineering Food for All." The New York Times. The
         New York Times, 19 Aug. 2011. Web. 16 May 2012. <http://www.
        nytimes.com/2011/08/19/opinion/genetically-engineered-food-for-
        all.html>.
 Giorgio, V. "Genetically Modified Food." Scienceray. Scienceray, 5 Mar.
        2008. Web. 16 May 2012. <http://scienceray.com/technology
        /applied-science/genetically-modified-food/>.
Genetically Modified Food!!!

Genetically modified foods

  • 1.
    Genetically Modified Foods By: Emily Luti
  • 2.
    How does itwork?  Genes use messages to make certain enzymes.  By introducing a foreign gene, new enzymes are made according to that gene. http://www.youtube.com/watch?v=RoCII huLl8A&feature=related&safety_mode=tr ue&persist_safety_mode=1&safe=active
  • 3.
    Techniques  Bacterial Carriers:  Bacterium such as Agrobacterium, is able to transfer DNA well to plants.  Bacterium prepared in special solution to make cell walls more permeable.  Selected gene is placed in the extra-chromosomal DNA (plasmid) of other bacterium and dropped into solution.  Solution is heated, giving the plasmid access to the Agrobacterium, and the gene begins to express itself.  The genetically altered bacterium is allowed to grow, then infect plants for the gene to be expressed.
  • 4.
    Techniques  Biolistics  Selected DNA is attached to small particles of either gold or metal tungsten.  DNA particles are shot into the target cells using pressure.
  • 5.
    Techniques  Calcium Phosphateprecipitation  Selected DNA is exposed to calcium phosphate, tiny granules are created.  Target cells respond to granules, and surround and digest them.  This allows the granules to release the DNA and deliver it to the host Nuclei and its DNA.
  • 6.
    Techniques  Electroporation  Prepared cells are surrounded by a special solution with the selected DNA.  A short electric shock is passed through the solution.  The result is a permeable cell wall for the DNA to enter the nuclei through.  Cells are placed into another solution which encourages to repair of their cell walls, locking the foreign DNA within.  The new DNA is incorporated into the chromosomes, and the host has a new gene.
  • 7.
    Techniques  Gene Silencing  The gene that controls the undesirable trait is identified.  A second copy of the gene, facing the wrong way around, is attached to the undesirable gene.
  • 8.
    Techniques  Gene Splicing  Restriction enzymes can cut their DNA into “sticky” fragments and paste them directly to another set of DNA for infection.  These restriction enzymes are used by scientists to genetically engineer cells. The DNA is cut, then inserted into a different set of DNA, where DNA ligase is used to fuse the new gene sequence to the chromosome.  Alternatively, the gene may be placed into a bacterial plasmid and allowed to enter the defective cell and deliver the new gene.
  • 9.
    Techniques  Lipofection  Small bubbles of fat, liposomes, are used to carry the specific DNA.  The target cells and liposomes are put into a special solution.  The liposomes combine with the phospholipids in the cell membrane, allowing the DNA to enter the cell and combine with the chromosomes.
  • 10.
    Techniques  Microinjection  Selected DNA is inserted into a female ovarian egg through a glass capillary tube.  The egg is transferred into the prepared uterus of a female to grow full term.  Creates a transgenic animal that will have all new cells.
  • 11.
    Techniques  Viral Carriers  Selected DNA is added to a virus.  Virus is then allowed to infect the target.  As the virus replicates inside the invaded cell, the DNA is added to the cells genetic make-up.
  • 12.
    Industry  Countries using:  Japan  Malaysia  Australia  Europe (pretty much everyone)  USA  Canada  Most first world countries and some second world  Most countries require labels for consumer choice
  • 13.
    Examples?  Plants: Rice  Soybean  Sugar Cane  Tomatoes  Corn  Potatoes  Peas  Squash,ect.
  • 14.
    Examples?  Meats  Cattle  Pigs  Other products  Honey  Cotton  Oils  Tobacco  Dairy  Vitamins
  • 15.
    The Debate: Goodor Bad?  Advantages  Medical Advances  Edible Vaccines  Virtual End to World Hunger  No mal nutrition  Grown in bulk/ plenty supply  Cheaper/Faster to Grow  Don’t have to be rich to plant  Environmentally Friendly  No pesticides  No run-off into water supply  Sustainable  Flood/Drought tolerant  Better Nutrition  Vitamin enriched  Better Bang for your Buck  Grown bigger  Endless possibilities  Anything alive can be genetically modified
  • 16.
    The Debate: Goodor Bad?  Disadvantages  Opposition  Religion  Regulation/ money cost for approvement  What will it do in the future?  Health risks  Allergies  Help or not?  Medicine  Antibiotic resistance  What could it create?  Resistant weeds  Pollination  Cross pollination in wild  Choice?  Labeling/ Health approval in some countries  Incidents already occurring  1989 incident killed dozens with infected food supplement L-tryptophan  2006 contaminated exported rice genes
  • 17.
    Autobiography  Butcher, Mavis."Genetically Modified Food-GM Foods List and Information." Disabled-World.com. Disabled-World, 22 Sept. 2009. Web. 16 May 2012. <http://www.disabled-worl.com/fitness/gm- foods.php>.  Deakin University- Faculty of Health and Behavioral Sciences. "Genetically Modified Foods-Techniques.“ Better Health Channel. State of Victoria. Web. 10 May 2012. <http://www.betterhealth.vic.gov.au /bhcv2/bhcarticles.nsf/pages/Genetically_modified_foods_techniques>.  Fedoroff, Nina V. "Engineering Food for All." The New York Times. The New York Times, 19 Aug. 2011. Web. 16 May 2012. <http://www. nytimes.com/2011/08/19/opinion/genetically-engineered-food-for- all.html>.  Giorgio, V. "Genetically Modified Food." Scienceray. Scienceray, 5 Mar. 2008. Web. 16 May 2012. <http://scienceray.com/technology /applied-science/genetically-modified-food/>.
  • 18.